Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in vitro

Tari, Kaveh; Atashi, Amir; Kaviani, Saied; AkhavanRahnama, Mahshid; Anbarlou, Azadeh; Mossahebi-Mohammadi, Majid

doi:10.1016/j.biologicals.2016.10.010

Cited by 10 publications

(8 citation statements)

References 19 publications

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“…It suggests a role of HGF in cardiac compensatory processes and also, the possibility that cardiac tissue might contribute to increased HGF plasma levels in PH. One of possible alternative mechanisms of plasma HGF elevation may involve influence of erythropoietin, another prognostic indicator of cardiovascular mortality 33, which plays a role in PH development 34 and it can increase Hgf expression in stem cells 35. Importantly, plasma levels of erythropoietin are known to be increased in PH patients 36 and MCT-injected rats as we reported previously 37.…”

Section: Discussionmentioning

confidence: 77%

Hepatocyte growth factor plays a particular role in progression of overall cardiac damage in experimental pulmonary hypertension

et al. 2019

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Background : HGF/MET pathway may have a role in pulmonary hypertension (PH). However, the link between the pathway and development of target organ damage in PH remains elusive. We aimed to demonstrate the relation between plasma HGF and HGF/MET tissue expressions in affected organs during PH progression. Methods : 12 weeks old male Wistar rats were injected with monocrotaline (MCT, 60 mg/kg, s.c.) to induce PH and sacrificed after 1, 2 and 4 weeks. Controls received saline. mRNA levels of HGF regulatory complex (Hgf, Met, Hgfa, Hai-1, Hai-2) were determined in right and left ventricles (RV, LV), lungs, pulmonary artery and liver by RT-qPCR. HGF protein levels in plasma were analysed by ELISA. Results : PH development was associated with a progressive elevation of HGF plasma levels that correlated with relative RV mass. Furthermore, Hgf mRNA expressions at week 4 were upregulated solely in the cardiac ventricles while being downregulated in a. pulmonalis , lungs and liver. Met and Hai-1/Hai-2 followed a similar pattern and were upregulated in cardiac ventricles, where Hgfa remained unchanged, but downregulated in lungs. Conclusion : We suggest that cardiac overexpression of Hgf might contribute to increased plasma HGF in MCT-induced PH. HGF could be exploited as a cardiospecific biomarker and HGF/MET pathway as a target in drug discovery for PH.

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Section: Discussionmentioning

confidence: 77%

Hepatocyte growth factor plays a particular role in progression of overall cardiac damage in experimental pulmonary hypertension

et al. 2019

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“…EPO-treated MSCs can be the main source of HGF production. Therefore, HGF can be used in the cultivation as a factor stimulating migration and as a mobilizing agent for HSC (Tari et al 2017).…”

Section: Erythropoietin (Epo)mentioning

confidence: 99%

Pharmacological modulation of cell functional activity with valproic acid and erythropoietin

Golubinskaya¹,

Sarycheva²,

Burda³

et al. 2019

RRP

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Introduction: Valproic acid (VA) is carboxylic acid with a branched chain, which is used as an antiepileptic drug. Valproic acid influence on cells in vivo: VA, which is an antiepileptic drug, is also a teratogen, which causes defects of a neural tube and an axial skeleton, although the mechanisms are not yet fully clear. Valproic acid influence on mesenchymal stem cells (MSC) in vitro: It is shown that valproic acid reduces the intracellular level of oxygen active forms. Valproic acid effect on tumor cells: VA inhibits tumor growth through several mechanisms, including the cell cycle stop, differentiation induction and inhibition of growth of tumor vessels. Valproic acid influence on enzymes: It affects mainly GSK-3. Valproic acid influence on animals’ cells: It is shown that VA can significantly improve an ability to develop in vitro and improve nuclear reprogramming of embryos. Erythropoietin (EPO): Is an hypoxia-induced hormone and a cytokine, which is necessary for normal erythropoiesis. EPO is widely used in in vitro experiments. Conclusion: Thus, the influence of VA and EPO on cells can be used in cell technologies.

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“…Recently, alternative EPO receptors (EphB4 5 , CD131 6 , and CRFL3 7 ) have been described, opening new pathways for EPO action. EPO has been shown to also target hematopoietic niche cells (osteoblasts and osteocytes 8,9 , endothelial cells 10,9 , adipocytes 11,12 , mesenchymal stem cells 13,14 ). In the hematopoietic hierarchy, EPO has also been suggested to act on hematopoietic stem and progenitor cells 15,13,16,17,18,19,20,21 , but several aspects remain debated.…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, as most studies analyzed the effect of in vivo EPO exposure on HSCs 8,9,10,11,12,13,14 , it is still unclear whether EPO acts directly on HSCs, via their environment, or both. To address the direct effects of EPO on HSC differentiation after transplantation at the clonal level, we here utilize cellular barcoding technology which allows us tracing of the progeny of hundreds of single HSCs in vivo.…”

Section: Introductionmentioning

confidence: 99%

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells

Eisele

Cosgrove

Magniez

et al. 2020

Preprint

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The cytokine erythropoietin (EPO) is a potent inducer of erythrocyte development and one of the most prescribed biopharmaceuticals. The action of EPO on erythroid progenitor cells is well established, but its action on hematopoietic stem cells (HSCs) is still debated. Here, using cellular barcoding, we traced the differentiation of hundreds of single HSCs, after in vitro EPO exposure and transplantation, in five different hematopoietic cell lineages, and observed the occurrence of high-output Myeloid-Erythroid-megaKaryocyte (MEK)-biased and Myeloid-B-cell-Dendritic cell (MBDC)-biased clones. ScRNAseq analysis of in vitro EPO-exposed HSCs revealed upregulation of erythroid associated genes in a subset of HSCs. Collectively, as well as demonstrating a direct effect of EPO on HSCs, our results suggest an EPO-mediated induction of MEK-bias in multi-outcome HSCs, and that this change in output is functionally compensated by MBDC-biased HSCs.

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Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in vitro

Cited by 10 publications

References 19 publications

Hepatocyte growth factor plays a particular role in progression of overall cardiac damage in experimental pulmonary hypertension

Hepatocyte growth factor plays a particular role in progression of overall cardiac damage in experimental pulmonary hypertension

Pharmacological modulation of cell functional activity with valproic acid and erythropoietin

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells

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