Erythropoietin improves hypoxic-ischemic encephalopathy in neonatal rats after short-term anoxia by enhancing angiogenesis

Yan, Fangfang; Meimei, Zhang; Yang, Meng; Li, Huijuan; Yu, Lie; Fu, Xiaojie; Tang, Youcai; Jiang, Chao

doi:10.1016/j.brainres.2016.09.024

Cited by 28 publications

(28 citation statements)

References 67 publications

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“…It was shown that both male and female rats exposed to neonatal anoxia exhibit a lower weight gain up to adulthood (Fig. 1A), thus confirming results of previous studies using similar neonatal anoxia models (Shimomura and Ohta, 1988; Tang and Nakazawa, 2005; Grojean et al, 2003; Yan et al, 2016). Different models of neonatal oxygen deprivation (e.g., asphyxia, hypoxia and hypoxia‐ischemia models) have also found reduction of weight gain (Andiné et al, 1990; Lubics et al, 2005; Hill et al, 2011; Ren et al, 2017; Grafe, 1994; Fleiss et al, 2011).…”

Section: Discussionsupporting

confidence: 88%

“…Growth and body weight modifications in children with neurodevelopmental disorders following oxygen deficiency have been reported (Lip et al, 2018;Brooks et al, 2011;Strand et al, 2016). Likewise, previous experimental studies have showed that neonatal oxygen deprivation impairs the somatic development of Wistar rats (Kumar et al, 2019;Shimomura and Ohta, 1988;Tang and Nakazawa, 2005;Grojean et al, 2003;Yan et al, 2016). However, little is known about the causes of these alterations.…”

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confidence: 99%

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Neonatal anoxia impairs long‐term energy metabolism and somatic development of Wistar rats

Cruz-Ochoa

Ochoa-Amaya

Pulecio

et al. 2019

Intl J of Devlp Neuroscience

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Background: Neonatal anoxia may cause neurological injuries, behavioral alterations and changes in somatic growth. Somatic developmental changes suggest a possible effect of anoxia on energy metabolism and/or feeding behavior. Short-term effects of oxygen deficit on energy homeostasis have been described. In contrast, just a few studies report long-term effects. This study investigated the effects of neonatal anoxia on energy metabolism and somatic development at adulthood of males and females Wistar rats. Method: Male (m) and female (f) rats were exposed, on postnatal day 2 (P2), to either 25-min of Anoxia or Control treatment. At P34 part of the subjects of each group was fasted for 18 h, refeed for 1 h and then perfused 30 min later, at P35; the remaining subjects were submitted to these treatments at P94 and perfused at P95. Therefore, there were 8 groups: AmP35, AmP95, AfP35, AfP95, CmP35, CmP95, CfP35 and CfP95. For subjects perfused at P95, body weight and food intake were recorded up to P90. For subjects perfused at P35 and P95, glycemia, leptin and insulin were assessed after fasting and refeed. After perfusion the encephalon and pancreas were collected for Fos immunohistochemistry and Hematoxylin-Eosin stain analyses. Results: Even though neonatal anoxia did not interfere with regular food intake, it reduced body weight gain along growing in both male and female subjects as compared to the corresponding controls. At P35 neonatal anoxia decreased post-prandial glycemia and increased insulin. While at P95 neonatal anoxia altered the pancreatic histomorphology and increased post-fasting weight loss, decreasing leptin, insulin and glycemia secretion, as well Fos immunoreactivity (IR) in ARC. Conclusion: Neonatal anoxia impairs long-term energy metabolism and somatic development in Wistar rats, with differences related to sex and age.

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Section: Discussionsupporting

confidence: 88%

mentioning

confidence: 99%

Neonatal anoxia impairs long‐term energy metabolism and somatic development of Wistar rats

Cruz-Ochoa

Ochoa-Amaya

Pulecio

et al. 2019

Intl J of Devlp Neuroscience

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“…Increased VEGF expression and activated VEGF/VEGFR-2 signaling pathway after rhEPO administration also resulted in an improvement of brain repair after anoxia. Indeed, rhEPO enhances angiogenesis, reduces white matter damage, and promotes cognitive recovery in anoxia rats [ 72 ]. Furthermore, EPO mediates neurovascular remodeling and neurobehavioral recovery in rats after traumatic brain injury via increased brain VEGF expression and phosphorylation of VEGFR-2 [ 73 ].…”

Section: Introductionmentioning

confidence: 99%

Erythropoietin and Its Angiogenic Activity

Kimáková

Solár

Solárová

et al. 2017

IJMS

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Erythropoietin (EPO) is the main hematopoietic hormone acting on progenitor red blood cells via stimulation of cell growth, differentiation, and anti-apoptosis. However, its receptor (EPOR) is also expressed in various non-hematopoietic tissues, including endothelium. EPO is a pleiotropic growth factor that exhibits growth stimulation and cell/tissue protection on numerous cells and tissues. In this article we review the angiogenesis potential of EPO on endothelial cells in heart, brain, and leg ischemia, as well as its role in retinopathy protection and tumor promotion. Furthermore, the effect of EPO on bone marrow and adipose tissue is also discussed.

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“…KEGG enrichment analysis (Table 4). Interesting, both of these pathway arereportedly involved in HI induced brain damage [39,40].…”

Section: Gene Ontology and Kegg Enrichment Analysismentioning

confidence: 99%

Circular RNA Expression in the Brain of a Neonatal Rat Model of Periventricular White Matter Damage

Zhu

Zhao

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Periventricular white matter damage (PWMD) is the predominant neurologic lesion in preterm infants who survive brain injury. In this study, we assessed the global changes in and characteristics of the transcriptome of circular RNAs (circRNAs) in the brain tissues of rats with PWMD. Methods: We compared the expression profiles of circRNAs in brain samples from three rats with PWMD and three paired control tissues using deep RNA sequencing. Bioinformatics analysis was applied to investigate these differentially expressed circRNAs, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was performed to confirm the results. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict associated cell signaling pathways and functions. Network analysis was performed to predict circRNAs-microRNAs, and target genes related to PWMD. Results: A total of 2151 more reliable circRNAs were dysregulated in the brain tissues of rats with PWMD, indicating a potential role in the condition. Of the 98 circRNAs significantly differentially expressed in rat brains with PWMD (P< 0.05), 52 were significantly over-expressed and 46 were significantly under-expressed. The expression profiles of seven of 10 randomly selected circRNAs were confirmed by qRT-PCR analysis. The glutamatergic synapse pathway and the VEGF signaling pathway, both associated with hypoxia/ischemia induced brain damage, were inriched. Relationship between miRNA (rno-miR-433-3p and rno-miR-206-3p) and HIF-1α were evident and potential associations between chr6: 48820833|48857932 and their target genes (rno-miR-433-3p and rno-miR-206-3p) were identified. Conclusion: The distinct expression patterns of circRNAs in the brain tissues of rats with PWMD suggest that circRNAs actively respond to hypoxia-ischemia. These findings could assist the development of novel diagnostic and therapeutic targets for PWMD therapy.

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Erythropoietin improves hypoxic-ischemic encephalopathy in neonatal rats after short-term anoxia by enhancing angiogenesis

Cited by 28 publications

References 67 publications

Neonatal anoxia impairs long‐term energy metabolism and somatic development of Wistar rats

Neonatal anoxia impairs long‐term energy metabolism and somatic development of Wistar rats

Erythropoietin and Its Angiogenic Activity

Circular RNA Expression in the Brain of a Neonatal Rat Model of Periventricular White Matter Damage

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