Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear

Cayé‐Thomasen, Per; Wagner, Niels; Frederiksen, Birgitte; Asal, Korhan; Thomsen, Jakob Borup

doi:10.1016/j.heares.2004.11.017

Cited by 23 publications

(33 citation statements)

References 32 publications

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“…Caye-Thomasen et al [30] have reported that EPO receptor is widely expressed by several cell types within the guinea pig cochlea, and they argued that EPO could be used for the prevention of inner ear damage. Moreover, Monge et al [16] demonstrated the presence of EPO and its receptors in the organ of Corti and the stria vascularis.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Recombinant Human Erythropoietin against Cisplatin-Induced Ototoxicity

Doğan¹,

Olgun²,

Kırkım³

et al. 2015

Int Adv Otol

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OBJECTIVE:To investigate the potential protective effect of recombinant human erythropoietin (rhEPO) against cisplatin-induced ototoxicity. MATERIALS and METHODS:Twenty-eight Wistar albino rats were divided randomly into four groups, and baseline distortion product otoacoustic emission (DPOAE) responses and auditory brainstem response (ABR) thresholds were obtained. Group I received intraperitoneal (i.p.) saline, Group II received a single dose of i.p. 2000 IU/kg rhEPO, and Group III and Group IV received a single dose of i.p. 16 mg/kg cisplatin injection. In Group IV, 2000 IU/kg rhEPO was also injected intraperitoneally two times: 24 hours before and 30 minutes after i.p. 16 mg/kg cisplatin injection. DPOAEs and ABR measurements were repeated 72 hours following cisplatin administration, and the animals were sacrificed. The cochleae of animals were evaluated histopathologically by light microscopy, and percentage of apoptotic cells in the spiral ganglion was then determined by immunofluorescence analysis. RESULTS:Post-treatment auditory assessment revealed significant ABR threshold elevations at click and 6 kHz and 8 kHz frequencies in Group III compared to Groups I and II (p<0.05). In Group III, 8 kHz DPOAEs were also significantly deteriorated compared to Groups I and II (p<0.05). In Group IV, the ABR thresholds and DPOAEs were protected at click and 6 kHz; there was no statistically significant difference between Group IV and Group I at those frequencies. Concomitant administration of rhEPO and cisplatin significantly reduced apoptosis and cell damage. The apoptotic cell percentage of spiral ganglions in Group IV was significantly less than in Group III (p=0.01). CONCLUSION:Our results showed that i.p. application of rhEPO inhibited apoptosis and prevented cisplatin-induced ototoxicity in rats.

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Section: Discussionmentioning

confidence: 99%

The Protective Effect of Recombinant Human Erythropoietin against Cisplatin-Induced Ototoxicity

Doğan¹,

Olgun²,

Kırkım³

et al. 2015

Int Adv Otol

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“…Cayé-Thomasen et al [18] found for the first time expression of Epo and its receptor in the inner ear. Epo expression was exclusively found in the supporting cells of the organ of Corti.…”

Section: Erythropoietin In the Cochleamentioning

confidence: 99%

Erythropoietin but not VEGF has a protective effect on auditory hair cells in the inner ear

Naldi

Gassmann

Bodmer

2009

Cell. Mol. Life Sci.

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“…Bars50 μm in guinea-pig retinal tissue, as it had not previously been characterised in this species. The anti-EPO and anti-EPOR antibodies were previously used for immunohistochemistry in the guinea-pig inner ear (Caye- Thomasen et al 2005), developing mouse midbrain (Knabe et al 2004) and adult human retina (Garcia-Ramirez et al 2008). In Western blot analysis, the EPO antibody recognised a protein at approximately 34 kDa in the P7 guinea-pig retina (Fig.…”

Section: Specificity Of Epo and Epor Antibodymentioning

confidence: 99%

Developmental profile of erythropoietin and its receptor in guinea-pig retina

et al. 2009

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Evidence suggests that endogenous erythropoietin (EPO) is involved in the development of the central nervous system; however, its role in retinal development is yet to be determined. In this study, we have used fluorescence immunohistochemistry to localise EPO and its receptor (EPOR) in the developing and mature retina of the guinea-pig, a species in which retinal development is similar to that in humans. EPO immunoreactivity (IR) was observed in ganglion cells from 25 days of gestation (dg; term approximately 67 dg), and in the inner and outer plexiform layers and in horizontal cells by 40 dg. EPO-IR persisted in all of these structures into adulthood. Müller cells also displayed EPO-IR, which was seen in the radial processes and endfeet at 40 dg and in the cytoplasm by 50 dg. IR in these cells was particularly intense and appeared to increase with age. EPOR-IR was found in all ages examined; it was detected in ganglion cells at 25 dg and, from 30 dg onwards, was localised on, and adjacent to, the cell surface membrane. The distribution of EPOR-IR became increasingly widespread during gestation and, by 50 dg, EPOR-IR was detectable on the majority of retinal somal membranes. This localisation persisted in the postnatal and adult retina. Therefore, IR for EPO and its receptor is present in the guinea-pig retina from as early as 25 dg, when retinal layers are forming, and persists throughout postnatal development. This suggests that EPO plays a role both in retinal development and in the maintenance of the adult retina.

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Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear

Cited by 23 publications

References 32 publications

The Protective Effect of Recombinant Human Erythropoietin against Cisplatin-Induced Ototoxicity

The Protective Effect of Recombinant Human Erythropoietin against Cisplatin-Induced Ototoxicity

Erythropoietin but not VEGF has a protective effect on auditory hair cells in the inner ear

Developmental profile of erythropoietin and its receptor in guinea-pig retina

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