Erythropoietic Response in Dogs Given Sublethal Whole-Body Proton Irradiation Followed by Hypoxic Hypoxia

Aceto, H.; Springsteen, R.W.; Gee, W; Winchell, H. S.; Tobias, Cornelius A.

doi:10.2307/3572633

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…To our knowledge, none of these past studies assessed either the effect of potentially lethal doses of protons on hematopoietic cells, organs and animal survival or any countermeasures (preventative or treatment) aside from shielding (16). Older studies did assess the hematopoietic effects of proton TBI in dogs and primates along with shielding or hypoxia as countermeasures (37-42). Our previous in vivo studies with γ rays, protons or HZE particles in mice and rats suggested that dietary supplementation with antioxidants is an effective countermeasure to prevent ionizing radiation-induced decreases in plasma total antioxidant status (a marker of oxidative stress) (18, 19).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Dietary Antioxidants on Proton Total-Body Irradiation-Mediated Hematopoietic Cell and Animal Survival

Wambi¹,

Sanzari²,

Sayers³

et al. 2009

Radiation Research

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Dietary antioxidants have radioprotective effects after γ-radiation exposure that limit hematopoietic cell depletion and improve animal survival. The purpose of this study was to determine whether a dietary supplement consisting of L-selenomethionine, vitamin C, vitamin E succinate, α-lipoic acid and N-acetyl cysteine could improve survival of mice after proton totalbody irradiation (TBI). Antioxidants significantly increased 30-day survival of mice only when given after irradiation at a dose less than the calculated LD 50/30 ; for these data, the dose-modifying factor (DMF) was 1.6. Pretreatment of animals with antioxidants resulted in significantly higher serum total white blood cell, polymorphonuclear cell and lymphocyte cell counts at 4 h after 1 Gy but not 7.2 Gy proton TBI. Antioxidants significantly modulated plasma levels of the hematopoietic cytokines Flt-3L and TGFβ1 and increased bone marrow cell counts and spleen mass after TBI. Maintenance of the antioxidant diet resulted in improved recovery of peripheral leukocytes and platelets after sublethal and potentially lethal TBI. Taken together, oral supplementation with antioxidants appears to be an effective approach for radioprotection of hematopoietic cells and improvement of animal survival after proton TBI.

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