Erythrocytic Committed Hematopoietic Stem Cells in the Peripheral Blood of Mice

McCarthy, Kenneth F.; MacVittie, Thomas J.

doi:10.1159/000208187

Cited by 4 publications

(1 citation statement)

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“…Adult mice, homozygous for the 'steel' locus, are characterized by severe macrocytic normochromic anaemia, lack of hair pigmentation and sterility. The observations that SI/Sld mice have an essentially nonerythropoietic spleen, a quantitative deficiency of early erythrocytic precursors in spleen, bone marrow (Wiktor-Jedrzejczak ef al., 1979) and blood (McCarthy & MacVittie, 1975) and a defective microenvironment for macroscopic erythrocytic spleen colonies (McCulloch ef al., 1965;Wolf, 1974) in conjunction with high serum erythropoietin levels (Bernstein, Russell & Keighley, 1968) have supported the view that the major disruption of erythropoiesis is early and more severe than in the granulocytic cell population (McCarthy, Ledney & Mitchell, 1973;Cole et al, 1975).…”

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Characteristics of the Cfu‐S Population In Mice Carrying the Sl^J Allele

Ploemacher

Brons

1984

Cell Proliferation

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Abstract. A tentative characterization of haemopoietic stem cells with respect to their organ distribution, seeding fraction and colony formation in the spleen, radiosensitivity and humoral regulation was attempted in mice heterozygous for the mutant allele Slj and in their normal littermates. SIJ/+ mice were characterized by a deficient CFU-s content of the blood and spleen and had slightly lower femoral CFU-s numbers. This CFU-s distribution could not be explained by differences in seeding efficiency 'f' between CFU-s of Sli/+ and +/+ origin in lethally irradiated recipients used in the CFU-s assay. The seeding fraction of CFU-s of +/+ origin did not differ in +/+ and Slj/+ recipients. However, in irradiated SIJ/+ recipient mice a 30% decrease was observed in the number of the colonies derived from splenic and femoral CFU-s of both +/+ and Slj/+ origin. The serum level of SHSF (splenic haemopoiesis stimulating factor) was decreased in SIJ/+ mice, but significantly increased in Sl/Sld mice, as compared to their respective normal + /+ littermates. Endogenous colony formation in SIJ/+ spleens was deficient in comparison to that observed in +/+ spleens, and distinct sex differences were observed. However, mutant and normal CFU-s from spleen and bone marrow had a similar survival following in-vitro y irradiation.Femurs and spleens of both Slj/+ and +/+ origin were implanted into both Slj/+ and +/+ hosts. Six weeks later the SlJ/+ grafts contained less CFU-s than the +/+ grafts.These data show that the splenic stroma of SlJ/+ mice is not defective in its capacity to lodge injected CFU-s but is deficient in its ability to maintain CFU-s under 'steady-state' conditions and stimulate their colony formation in a 'perturbed state'.

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