Erythrocytes volume and refractive index measurement with a digital holographic microscope

Rappaz, Benjamin; Barbul, Alexander; Charrière, Florian; Kühn, Jonas; Marquet, Pierre; Korenstein, Rafi; Depeursinge, C.; Magistretti, Pierre J.

doi:10.1117/12.700463

Cited by 11 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…13 The refractive index of cells was also varied from 1.387 to 1.401 according to the range from the literature. 14 The flow of cells was modeled on the basis of the incremental displacement (δy) of the cells through an observation plane. The shapes of the blood cells were approximated as biconcave discs and were assumed to be perfectly symmetric around their center.…”

Section: ■ Simulation Setup and Model Parametersmentioning

confidence: 99%

Statistical Analysis of Photonic Crystal Spectra for the Independent Determination of the Size and Refractive Index of Cells

Stewart

Pyayt

2015

Langmuir

View full text Add to dashboard Cite

Photonic crystal flow cytometry is a very attractive platform due to its great sensitivity in combination with a very compact design. Previous studies have demonstrated the possibility to use spectral processing for the measurement of a wide range of parameters, from simple object counting to independent analysis of the buffer solution and immersed microscale objects. Here we propose to go to the next level and simultaneously determine the shape and the refractive index of the cells.

show abstract

Section: ■ Simulation Setup and Model Parametersmentioning

confidence: 99%

Statistical Analysis of Photonic Crystal Spectra for the Independent Determination of the Size and Refractive Index of Cells

Stewart

Pyayt

2015

Langmuir

View full text Add to dashboard Cite

show abstract

“…al. 10,11 have demonstrated the Digital Holographic Microscopy (DHM) technique to obtain quantitative phase-mapping of living cell with interferometric method from a single recorded hologram exogenously followed by decoupling procedure, initially conceptualized by Barer 12 and, Evans and Fung 13 .…”

Section: Introductionmentioning

confidence: 99%

Interferometry for topographical diagnostics of RBCs in optical tweezers

et al. 2010

View full text Add to dashboard Cite

Simultaneous non-invasive trapping and topography of erythrocytes by interferometric fringe projection profilometry has been presented. Sinusoidal fringes were generated with the help of compact Michelson interferometer (CMI) developed by coating a thin reflective layer (~100µm) of Al 2 O 3 on one face of a cubic beam splitter. An external mirror was mounted on XYZ translational stage to control the fringe frequency and orientation. Red He-Ne laser was used to generate the sinusoidal probe -interferogram using CMI to project onto the green-laser (cw) trapped healthy and deceased RBCs separately. Information coded reflected interferograms exhibits characteristic fringe-deviation with respect to probe-pattern for both the healthy and defective RBCs. Fourier transform analysis was adopted to retrieve the phase-map which can be exploited for topography, size determination and refractive-index of RBC. Refractive-index change is directly related with hemoglobin concentration of RBCs at any specific physiological state and hence information about health status and disease progression can be anticipated.

show abstract

“…There are a lot of various type of cell were estimated using different methods of interference microscopy: erythrocytes (see e.g. Popescu et al, 2005;Popescu et al, 2006;Rappaz et al, 2007;Rappaz et al, 2008;Rappaz et al, 2009;Yusipovich, I et al, 2009;Yusipovich et al, 2011), fibroblasts (Dunn & Zicha, 1995), lymphocytes (Tychinsky et al, 2012), neurons (Rappaz et al, 2005;Yusipovich et al, 2006), nerve fibers (see e.g. Bibineyshvili et al, 2013;Brazhe et al, 2008;LaPorta & Kleinfeld, 2012;Maksimov et al, 2001), mast cells (Bondarenko et al, 2013), any microbiological objects (Tychinskii et al, 2007;Yusipovich et al, 2012;Zhurina et al, 2013) and others.…”

Section: Introductionmentioning

confidence: 99%

Application of Phase Images for Estimation of Peritoneal Macrophages State

Yusipovich¹,

Baizhumanov²,

Казакова³

et al. 2015

IJB

View full text Add to dashboard Cite

Procedure based on phase images obtained by the method of Laser Interference Microscopy (LIM) for detection of number non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro was provided. The phase images provide quantitative estimates of the optical path difference (OPD) in each point of object. The OPD varies with cell thickness and concentration of cellular substances. Therefore, it provides useful information on cell structure, localization of subcellular structures, local concentrations of substances and dry mass of cell. In these work we propose a new assay for estimation a number of non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro based on cell phase images. The results were compared with phagocytic index, which was evaluated using latex beads, and the percentage of cells with damaged membranes (dead cells). It was shown that with increasing time after the isolation, the percentage of dead macrophages estimated by LIM was similar to that of the cells with damaged membranes. However, the percentage of activated cells calculated using latex beads exceeded the percentage calculated using the phase images significantly.

show abstract

Erythrocytes volume and refractive index measurement with a digital holographic microscope

Cited by 11 publications

References 9 publications

Statistical Analysis of Photonic Crystal Spectra for the Independent Determination of the Size and Refractive Index of Cells

Statistical Analysis of Photonic Crystal Spectra for the Independent Determination of the Size and Refractive Index of Cells

Interferometry for topographical diagnostics of RBCs in optical tweezers

Application of Phase Images for Estimation of Peritoneal Macrophages State

Contact Info

Product

Resources

About