Erythrocytes as carriers of metronidazole: In-vitro characterization

Talwar, Naresh; Jain, Narendra Kumar

doi:10.3109/03639049209040903

Cited by 38 publications

(24 citation statements)

References 10 publications

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“…This method is based on the principle of first swelling the erythrocytes without lysis by placing them in slightly hypotonic solution. Drugs encapsulated in erythrocytes using this method include propranolol [33], asparginase [34], cyclophosphamide, cortisol-21-phosphate [32], 1-antitrypsin [27], methotrexate [27], insulin [35], metronidazole [36], levothyroxine [37], enalaprilat [38], and isoniazide [39]. Then, relatively small volumes of aqueous drug solution are added to the point of lysis.…”

Section: C) Preswell Methodsmentioning

confidence: 99%

Drug Loaded Erythrocytes: As Novel Drug Delivery System

Patel¹,

Dand²,

Hirlekar³

et al. 2008

CPD

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Novel drug delivery systems are one of the widely used delivery systems. In the present scenario, amongst them, "Drug Loaded Erythrocytes" is one of the growing and potential systems for delivery of drugs and enzymes. Erythrocytes are biocompatible, biodegradable, possess long circulation half-life and can be loaded with variety of biologically active substances. Carrier erythrocytes are prepared by collecting blood sample from the organism of interest and separating erythrocytes from plasma. By using various physical and chemical methods the cells are broken and the drug is entrapped into the erythrocytes, finally they are resealed and the resultant carriers are then called "resealed erythrocytes". Surface modification with glutaraldehyde, antibodies, carbohydrates like sialic acid and biotinylation of loaded erythrocytes (biotinylated erythrocytes) is possible to improve their target specificity and to increase their circulation half-life. Upon reinjection the drug loaded erythrocytes serve as slow circulation depots, targets the drug to the reticuloendothelial system (RES), prevents degradation of loaded drug from inactivation by endogenous chemicals, attain steady state concentration of drug and decrease the side-effects of loaded drug. Nowadays, Nanoerythrosomes based drug delivery systems have excellent potential for clinical application.

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Section: C) Preswell Methodsmentioning

confidence: 99%

Drug Loaded Erythrocytes: As Novel Drug Delivery System

Patel¹,

Dand²,

Hirlekar³

et al. 2008

CPD

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show abstract

“…Several strategies have been attempted to overcome this problem with the use of membrane cross-linkers being the only successful one. [8][9][10][11][12] This strategy, however, suffers from the inclusion of foreign chemicals in the procedure, particularly, considering that these agents are potentially harmful to carrier cells life span in circulation via making their membranes more rigid compared with the normal cells. We, therefore, evaluated a novel strategy of using drug-loaded nanoparticles, instead of the "bare" drug for encapsulation in erythrocytes to solve the aforementioned problem.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

Hamidi

Rafiei

Azadi

et al. 2011

Journal of Pharmaceutical Sciences

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“…They have a long ∼120-day lifespan in circulation, and this feature can be exploited in applications where slow and sustained cargo release into the intravenous circulation is desired. For example, erythrocytes have been used as circulating depots of vitamins 3 and steroids, 4-7 agents for antineoplastic, [8][9][10] antiparasitic, [11][12][13] and antiretroviral [14][15][16][17][18][19][20] therapies, and also as carriers for cardiovascular therapeutics. [21][22][23] Erythrocyte carriers are intrinsically biodegradable without the generation of toxic byproducts.…”

Section: Introductionmentioning

confidence: 99%

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Atukorale

Bekdemir

et al. 2015

Nanoscale

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Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long lifespan in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto the membrane, all of which require ex vivo manipulation.Here, we characterized the properties of amphiphilic gold nanoparticles (amph-AuNPs), comprised of a ∼2.3 nm gold core and an amphiphilic ligand shell, which are able to embed spontaneously within erythrocyte membranes and might provide a means to load drugs into red blood cells (RBCs) directly in vivo. Particle interaction with RBC membranes occurred rapidly at physiological temperature. We further show that amph-AuNP uptake by RBCs was limited by the glycocalyx and was particularly influenced by sialic acids on cell surface proteoglycans. Using a reductionist model membrane system with synthetic lipid vesicles, we confirmed the importance of membrane fluidity and the glycocalyx in regulating amph-AuNP/ membrane interactions. These results thus provide evidence for the interaction of amph-AuNPs with erythrocyte membranes and identify key membrane components that govern this interaction, providing a framework for the development of amph-AuNP-carrying erythrocyte 'pharmacytes' in vivo.

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Erythrocytes as carriers of metronidazole: In-vitro characterization

Cited by 38 publications

References 10 publications

Drug Loaded Erythrocytes: As Novel Drug Delivery System

Drug Loaded Erythrocytes: As Novel Drug Delivery System

Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

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