Erythrocyte osmotic fragility test for screening of alpha‐thalassemia‐1 and beta‐thalassemia trait in pregnancy

Abstract

OFT has high sensitivity in detection of alpha-thalassemia-1 trait or beta-thalassemia trait and due to its simplicity with very low cost it may, therefore, be considered as a screening test in a wide population.

A Stopped Flow System with Hydrodynamic Injection for Red Blood Cells Osmotic Fragility Test: Possibility for Automatic Screening of Beta-thalassemia Trait

“…Here, 2.812 g of NaCl (Carlo Erba), 1.424 g of Na2HPO4·2H2O (BDH), 0.262 g of NaH2PO4·2H2O (Merck) and 16.744 g of glycerol (Carlo Erba) were dissolved in 1000 ml of deionized (DI) water. 10 The ionic strength of this hypotonic solution was calculated to be 0.074 and its pH was measured by a pH meter and was found to be 7.5.…”

“…The DNA study. 5,[8][9][10][11] normal, relatively impermeable, biconcave disc-like red cells can Among many thalassemia screening techniques, OFT is maintain osmotic equilibrium with the in-vivo surrounding recognized as an effective screening technique for thalassemia medium. When cells are placed in a hypotonic saline medium, triats commonly found in Thailand and in neighbouring countries; fluid will move into these cells by osmosis and cause them to i.e.…”

“…7 OFT is therefore used for identification of beta-thalassemia, in particular. [8][9][10] screening of some red blood cell-related diseases in human and…”

“…8,9 Various In developing countries with high rates of thalassemia, where saline concentrations (0.34, 0.36 and 0.45%) were reported budget, facilities and number of trained personnel are limited, effective by different groups. [8][9][10]15 Results are observed within 30 prevention of thalassemia is very important and it is necessary min. Normal red cells will break and yield clear red solution.…”

“…10 However, some steps such as manual mixing and placing a cuvette in a spectrometer may still cause imprecision. Therefore, an automatic system for kinetic OFT with higher accuracy and precision, one that is rapid and consumes relatively little amount of blood samples, should be beneficial.…”