Erythrocyte Na<sup>+</sup>/K<sup>+</sup>‐ATPase is increased in subjects with subclinical hypothyroidism

Nicolini, Giuseppina; Balzan, Silvana; Colzani, Raffaella; Scarlattini, M.; Taddei, Marco; Iervasi, Giorgio

doi:10.1111/j.1365-2265.2004.02039.x

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…These findings implicate that cinnabar can alter Na + /K + -ATPase activities of the brain and NO x levels of the whole blood, which maybe responsible for inducing the dysfunctions of nervous (abnormalities of locomotor activities and motor equilibrium performance) and central auditory system (elevation of hearing thresholds, and delay of absolute latency and interwave latency). In addition, recent studies have suggested that changes in Na + /K + -ATPase activities and/or NO x levels could be the useful biochemical markers for chemical-induced neuronal injuries or subclinical disease, especially in mercuric compounds-induced neurotoxicity [21, 53, 54]. Based on these suggestions and our findings, we suggest that Na + /K + -ATPase activities and NO x levels appear to serve as an important and useful biochemical marker of lower dose of cinnabar-induced neurotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

Huang

Hsu

Liu

et al. 2012

Journal of Biomedicine and Biotechnology

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Cinnabar, a naturally occurring mercuric sulfide (HgS), has long been used in Chinese mineral medicine for more than 2000 years. Although mercury is well-known for its toxicity, whether cinnabar induces neurotoxicity, especially in infants and children, is unknown. The purpose of this study was to explore the neurotoxic effects of low-dose of cinnabar (10 mg/kg/day) on developing mice. The results revealed neurobehavioral defects in F1-C-Cin group, which were associated with Hg accumulation, increased NOx levels in whole blood, and Na+/K+-ATPase activities in brain tissues. F1- and F2-Cin-V groups were found to increase brain Hg contents and prominent neurobehavioral defects compared with F1-C-V group, suggesting that the fetal brain was more susceptible to irreversible effects for cinnabar-induced damage. Moreover, F1- and F2-Cin-Cin groups had severely neurobehavioral dysfunctions, closely correlated with the further alteration of NOx levels and Na+/K+-ATPase activities than F1- and F2-C-Cin groups. Effects in F2-Cin-Cin group were more significant than those in F1-Cin-Cin group. In conclusion, this study demonstrates that exposure to low-dose of cinnabar during the perinatal and developmental stages results in irreversible and severe injuries of the neurotoxicity in offspring, and NOx and Na+/K+-ATPase activities may exist potential and useful biomarkers for neurotoxicity-induced by low-doses of mercuric compounds.

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Section: Discussionmentioning

confidence: 99%

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

Huang

Hsu

Liu

et al. 2012

Journal of Biomedicine and Biotechnology

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“…Hence, sodium pump impairment may be a plausible explanation for the neurotoxicity due to improper intrauterine development that has been observed in hypothyroid patients. However, some earlier reports have suggested contrasting results, reporting that increased levels of sodium pump activity were associated with decreased thyroid function, both in overt as well as in subclinical cases [1920]. These inconsistent results suggest that no uniform alterations occur in the function of the sodium pump in thyroid disorders.…”

Section: Introductionmentioning

confidence: 94%

The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism

Roy

Dasgupta

2017

Endocrinol Metab

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BackgroundMetabolic dysfunctions characteristic of overt hypothyroidism (OH) start at the early stage of subclinical hypothyroidism (SCH). Na+/K+-ATPase (the sodium pump) is a transmembrane enzyme that plays a vital role in cellular activities in combination with membrane lipids. We evaluated the effects of early changes in thyroid hormone and membrane cholesterol on sodium pump activity in SCH and OH patients.MethodsIn 32 SCH patients, 35 OH patients, and 34 euthyroid patients, sodium pump activity and cholesterol levels in red blood cell membranes were measured. Serum thyroxine (T4) and thyroid stimulating hormone (TSH) levels were measured using enzyme-linked immunosorbent assays. Differences in their mean values were analysed using post hoc analysis of variance. We assessed the dependence of the sodium pump on other metabolites by multiple regression analysis.ResultsSodium pump activity and membrane cholesterol were lower in both hypothyroid groups than in control group, OH group exhibiting lower values than SCH group. In SCH group, sodium pump activity showed a significant direct dependence on membrane cholesterol with an inverse relationship with serum TSH levels. In OH group, sodium pump activity depended directly on membrane cholesterol and serum T4 levels. No dependence on serum cholesterol was observed in either case.ConclusionDespite the presence of elevated serum cholesterol in hypothyroidism, membrane cholesterol contributed significantly to maintain sodium pump activity in the cells. A critical reduction in membrane cholesterol levels heralds compromised enzyme activity, even in the early stage of hypothyroidism, and this can be predicted by elevated TSH levels alone, without any evident clinical manifestations.

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“…For example, in the study by Ref. [24], it was suggested that erythrocyte Na + /K + -ATPase would be a good biochemical marker for subclinical hypothyroidism, as it is sensitive to subtle changes in thyroid function in this situation [24].…”

Section: Figurementioning

confidence: 99%

Hormones Action on Erythrocytes and Signaling Pathways

Guimarães-Nobre¹,

Mendonça-Reis²,

Teixeira-Alves³

et al. 2023

The Erythrocyte - A Unique Cell

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Erythrocytes are the most abundant cell type in the human body, although considered as merely hemoglobin carriers for a long time. Extensive studies on its biochemical pathways, metabolism, and structure-activity relationship with a consistent number of publications demonstrated the presence of autocrine, paracrine, and endocrine hormone receptors. In this chapter, some of these hormones will be discussed, bringing attention to those that regulate erythrocyte survival, disease connection, and functionality.

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Erythrocyte Na⁺/K⁺‐ATPase is increased in subjects with subclinical hypothyroidism

Abstract: Erythrocytes of subclinically hypothyroid patients show a significant increase in the number of ouabain-binding sites and in ouabain-sensitive (86)Rb uptake. The state of erythrocyte Na(+)/K(+)-ATPase may therefore represent a biochemical marker of subclinical hypothyroidism.

Cited by 7 publications

References 27 publications

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism

Hormones Action on Erythrocytes and Signaling Pathways

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Erythrocyte Na+/K+‐ATPase is increased in subjects with subclinical hypothyroidism

Abstract: Erythrocytes of subclinically hypothyroid patients show a significant increase in the number of ouabain-binding sites and in ouabain-sensitive (86)Rb uptake. The state of erythrocyte Na(+)/K(+)-ATPase may therefore represent a biochemical marker of subclinical hypothyroidism.

Cited by 7 publications

References 27 publications

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

Exposure to Low Dose of Cinnabar (a Naturally Occurring Mercuric Sulfide (HgS)) Caused Neurotoxicological Effects in Offspring Mice

The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism

Hormones Action on Erythrocytes and Signaling Pathways

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Erythrocyte Na⁺/K⁺‐ATPase is increased in subjects with subclinical hypothyroidism