Erythrocyte Na+ -Li+ countertransport in essential hypertension

Villar, José R.; Montilla, C.; Muñiz-Grijalvo, Ovidio; Fg, Muriana; Stiefel, Pablo; Ruiz-Gutiérrez,; Carneado, J

doi:10.1097/00004872-199608000-00006

Cited by 25 publications

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“…22 Indeed, alterations of membrane cholesterol or phospholipid content, phospholipid distribution, the molecular species of particular phospholipid classes, and the degree of FA saturation have all been reported in both hypertensive humans 1,4,23 and in experimental animal models of hypertension. 2,24 Interestingly, we recently demonstrated that in elderly hypertensive patients, alterations in cell membrane lipid levels are also associated with a reduction in the density of signaling proteins involved in the control of BP, such as G proteins and PKC.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in the plasma or membrane lipid composition have been reported in both hypertensive humans 1 and in animal models of hypertension. 2,3 These changes have been associated with abnormalities in cation transport systems, 4 cytosolic Ca 2ϩ regulation, 5 and impaired signal transduction. 6 As a result, it has been proposed that modifications of the membrane lipid composition can affect the physical and functional properties of membranes and in consequence, might account for the alterations in signaling implicated in the physiological control of blood pressure (BP).…”

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confidence: 99%

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2-Hydroxyoleic Acid

et al. 2004

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Abstract-Recent studies have shown that diets rich in monounsaturated fatty acids (MUFAs) from olive oil, a natural source of oleic acid, have beneficial effects on blood pressure (BP) in hypertensive patients. With this in mind, we investigated whether a synthetic derivative of the MUFA oleic acid, 2-hydroxyoleic acid (2-OHOA), was capable of regulating the BP of Sprague-Dawley rats. Intraperitoneal and oral administration of 2-OHOA to rats induced significant and sustained decreases in BP in a time-dependent manner. Without affecting heart rate, treatments for 7 days provoked reductions in systolic BP of 20 to 26 mm Hg. At the molecular level, the density of G␣s, but not G␣i 2 or G␣o, increased in membranes from the hearts and aortas of 2-OHOA-treated rats, whereas in heart membranes, the density of G␣q/11 and protein kinase C␣ proteins was also augmented. These molecular alterations were reflected in the increase in cAMP levels after G␣s protein and ␤-adrenergic receptor stimulation. On the contrary, inhibitory hormones reduced adenylyl cyclase activity to the same extent in 2-OHOA-treated rats as in vehicle-treated ones. Our results indicate that cardiovascular tissues from 2-OHOA-treated rats exhibited increased cAMP production in response to G␣s activation, which might be attributed to enhanced expression of G␣s proteins. As a result of this change, a significant reduction in systolic BP was observed. Therefore, BP can be lowered by administration of 2-OHOA, which might represent the first member of a new family of antihypertensive drugs. Key Words: blood pressure Ⅲ fatty acids Ⅲ signal transduction Ⅲ G proteins Ⅲ hypotension M any alterations in the structure and function of the cell membrane have been associated with hypertension. Changes in the plasma or membrane lipid composition have been reported in both hypertensive humans 1 and in animal models of hypertension. 2,3 These changes have been associated with abnormalities in cation transport systems, 4 cytosolic Ca 2ϩ regulation, 5 and impaired signal transduction. 6 As a result, it has been proposed that modifications of the membrane lipid composition can affect the physical and functional properties of membranes and in consequence, might account for the alterations in signaling implicated in the physiological control of blood pressure (BP). In this context, long-term fatty acid (FA) intake is known to influence both membrane lipid composition and BP. Olive oil, a natural source of the monounsaturated FA (MUFA) oleic acid, has been shown to have beneficial effects on BP (when high doses are consumed for long periods) and is associated with a low incidence of coronary heart disease. 7 Olive oil consumption causes favorable changes in plasma lipid and lipoprotein profiles 8,9 and also in the phospholipid content and FA composition of both erythrocyte membranes from hypertensive patients and aorta membranes from spontaneously hypertensive rats. 10,11 Moreover, a slight reduction in saturated fat intake along with the use of olive oil markedly lowers the ...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

2-Hydroxyoleic Acid

et al. 2004

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“…The structural properties and function of cell membranes appear to be modified in hypertensive humans and animal models of hypertension. 8 -10 Changes in membrane lipid composition of hypertensive subjects have been associated with alterations in the transmembrane fluxes of Na ϩ and K ϩ , including Na ϩ -Li ϩ countertransport, which is a marker of essential hypertension, 10,11 and in cell signaling proteins that participate in the control of blood pressure. 12 On the other hand, it has been reported that dietary lipids have an effect on membrane lipid composition and cell signaling proteins.…”

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confidence: 99%

Mediterranean-Style Diet Effect on the Structural Properties of the Erythrocyte Cell Membrane of Hypertensive Patients

et al. 2009

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Abstract-A currently ongoing randomized trial has revealed that the Mediterranean diet, rich in virgin olive oil or nuts, reduces systolic blood pressure in high-risk cardiovascular patients. Here, we present a structural substudy to assess the effect of a Mediterranean-style diet supplemented with nuts or virgin olive oil on erythrocyte membrane properties in 36 hypertensive participants after 1 year of intervention. Erythrocyte membrane lipid composition, structural properties of reconstituted erythrocyte membranes, and serum concentrations of inflammatory markers are reported. After the intervention, the membrane cholesterol content decreased, whereas that of phospholipids increased in all of the dietary groups; the diminishing cholesterol:phospholipid ratio could be associated with an increase in the membrane fluidity. Moreover, reconstituted membranes from the nuts and virgin olive oil groups showed a higher propensity to form a nonlamellar inverted hexagonal phase structure that was related to an increase in phosphatidylethanolamine lipid class. These data suggest that the Mediterranean-style diet affects the lipid metabolism that is altered in hypertensive patients, influencing the structural membrane properties. The erythrocyte membrane modulation described provides insight in the structural bases underlying the beneficial effect of a Mediterranean-style diet in hypertensive subjects. Key Words: Mediterranean diet Ⅲ lipids Ⅲ membrane structure Ⅲ cardiovascular disease Ⅲ hypertension C ardiovascular disease constitutes the main cause of death in industrialized countries, 1 and hypertension is one of the main modifiable cardiovascular risk factors, especially in the elderly. 2 Healthy diet and lifestyle constitute the first steps in the guidelines for management of hypertension. 3 In this context, the type and amount of dietary lipids influence the lipid composition of cell membranes 4,5 and modulate the interactions with proteins involved in the regulation of blood pressure. 6 Thus, the changes in membrane properties induced by dietary lipids may have important consequences on the blood pressure regulation.The Mediterranean-style diet (MD) is characterized by a high consumption of virgin olive oil (VOO) and nuts, which are rich natural sources of oleic (18:1; n-9) and ␣-linolenic (18:3; n-3) acids, respectively. The Prevencion con Dieta Mediterranea (PREDIMED) Study is a large-scale, randomized trial aimed at assessing the effects of a MD enriched with VOO or nuts on primary prevention of cardiovascular disease in patients at high risk for coronary heart disease. The results of the 3-month intervention on the first 772 patients entering the study showed that, compared with a low-fat diet, the MD rich in VOO or nuts reduced systolic blood pressure and serum total cholesterol and triglyceride concentrations and increased serum high-density lipoprotein cholesterol concentration. 7 Although there is evidence indicating that dietary lipids can have a positive effect on cardiovascular risk factors, the mechani...

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“…For instance, other groups have repeatedly shown that serum triglycerides correlated with the activity of the Na ϩ -Li ϩ exchange in erythrocytes and therefore modified the relationship between this transport system and blood pressure. [38][39][40][41][42] Our findings are relevant in that they clearly show that triglycerides do not modify the interrelationships between the various cellular Ca 2ϩ (and Na ϩ ) parameters but the relationships between these parameters and blood pressure. In this regard, the variation in lymphocyte parameters might not be causally related to variation in triglycerides.…”

Section: Resultsmentioning

confidence: 56%