Erythrocyte Morphological Characteristics Based on Microscope Images System

Abstract

Microscope images analysis of erythrocyte (red blood cells, RBCs) was a widely used method for medical purpose. Usually manual measuring and analysis of the images were subject to time-consuming, errors and unstability of results. The images analysis method had been combined with computer image processing techniques in this research. A measuring and analysis system for microscope images(MIAS) of RBCs was developed, which could recognize RBCs in images and measure cells mophometric parameters. Normal human RBCs were compared with ones under high glucose. The results indicated RBCs sizes parameters such as areas, perimeters, major axis lengths, minor axis lengths, elongations, roundnesses and Feret diameters have difference between normal and high glucose conditions. RBCs normal disk shapes changed into acanthocyte and stomatocyte under higher glucose conditions. This fast and precise method for measuring RBCs morphometric parameters contributed to pathogenesis of diabetic nephropathy(DN) research.