Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization

Kostić, Ivana T.; Ilić, Vesna; Đorđević, Verica; Bukara, Katarina; Mojsilović, Slavko; Nedović, Viktor; Bugarski, Diana; Veljović, Djordje; Mišić, Danijela; Bugarski, Branko

doi:10.1016/j.colsurfb.2014.06.043

Cited by 23 publications

(52 citation statements)

References 41 publications

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“…However, the presence of these minor invaginations of DexP encapsulated ghosts compared to sham‐encapsulated ghosts were more pronounced in the case of porcine ghosts. This result was in accordance with already reported more susceptible nature of porcine erythrocyte ghosts upon exposure to hypoosmotic conditions …”

Section: Resultssupporting

confidence: 94%

“…Obviously, under the same experimental conditions approximately five times higher values of loaded DexP were obtained in the case of porcine compared to bovine erythrocyte ghosts. This result could be explained by difference in membrane lipids and proteins composition between the two types of cells . Namely, in mammals, phosphatidylcholine (PC) is generally the most abundant erythrocyte membrane phospholipid.…”

Section: Resultsmentioning

confidence: 99%

“…Blood of Holstein‐Friesian calves and Swedish Landrace swine was taken from jugular vein and collected in a sterile glass bottle containing 3.8% Na‐citrate as an anticoagulant agent. Preparations of packed erythrocytes, as well as determination of hemoglobin concentration, hematocrit, erythrocyte number, and remained hemoglobin content in the ghosts were performed as described in Kostić et al…”

Section: Methodsmentioning

confidence: 99%

“…A gradual hypotonic hemolysis method using a very simple set up was employed for DexP loading into bovine and porcine erythrocyte ghosts. For this purpose, 50 mL washed packed erythrocytes were suspended in isotonic PBS to an Hct of 60%, and poured in a 2 L glass beaker.…”

Section: Methodsmentioning

confidence: 99%

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Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate

Drvenica

Bukara

Ilić

et al. 2016

Biotechnology Progress

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The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P < 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1046-1055, 2016.

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate

Drvenica

Bukara

Ilić

et al. 2016

Biotechnology Progress

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“…Numerous studies show, that erythrocytes can be used as vehicles for bioactive agents, therapeutics can be loaded into the cells, or coupled onto their surfaces [9][10][11]. The advantage of this approach is that they are of natural origin, thereby serving as biocompatible carriers.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Deák

Mihály

Szigyártó

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Colloidal stabile nanoerythrosomes with 200 nm average diameter were formed from hemoglobin-free erythrocyte ghost membrane via sonication and membrane extrusion. The incorporation of extra lipid (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC), added to the sonicated ghosts, caused significant changes in the thermotropic character of the original membranes. As a result of the increased DPPC ratio the chain melting of the hydrated DPPC system and the characteristic small angle X-ray scattering (SAXS) of the lipid bilayers appeared. Significant morphological changes were followed by transmission electron microscopy combined with freeze fracture method (FF-TEM). After the ultrasonic treatment the large entities of erythrocyte ghosts transformed into nearly spherical nanoerythrosomes with diameters between 100-300 nm and at the same time a great number of 10-30 nm large membrane proteins or protein clusters were dispersed in the aqueous medium. The infrared spectroscopy (FT-IR) pointed out, that the sonication did not cause changes in the secondary structures of the membrane proteins under our preparation conditions. About fivefold of extra lipid -compared to the lipid content of the original membrane -caused homogeneous dispersion of nanoerythrosomes however the shape of the vesicles was not uniform. After the addition of about tenfold of DPPC, monoform and monodisperse nanoerythrosomes became typical. The outer surfaces of these roughly spherical objects were frequently polygonal, consisting of a net of pentagons and hexagons..

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Improved pharmacokinetic and biodistribution of 5-fluorouracil loaded biomimetic nanoerythrocytes decorated nanocarriers for liver cancer treatment

Alqahtani

Harisa

Alomrani

et al. 2021

Colloids and Surfaces B: Biointerfaces

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Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization

Cited by 23 publications

References 41 publications

Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate

Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Improved pharmacokinetic and biodistribution of 5-fluorouracil loaded biomimetic nanoerythrocytes decorated nanocarriers for liver cancer treatment

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