Erythrocyte Membrane Vesiculation and Changes in
Membrane Composition during Storage in
Citrate-Phosphate-Dextrose-Adenine-1

Greenwalt, Tibor J.; Bryan, Donna J.; Dumaswala, U.J.

doi:10.1159/000466301

Cited by 37 publications

(57 citation statements)

References 30 publications

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“…21,[31][32][33][34][35][36][37][38] It has also been previously suggested that surface expression of erythrocyte Duffy antigen is decreased with RBC storage and Duffy antigen has been identified on exocytic vesicles from stored erythrocytes. [39][40][41][42] Consistent with our findings, others have shown that banked human RBCs undergo loss of Duffy expression and chemokine scavenging function. 21 In banked human RBC units, we found that the overall reduction in surface Duffy expression is modest and may not fully explain the significant reductions in chemokine scavenging of CXCL1 and CCL2.…”

Section: Discussionsupporting

confidence: 92%

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

Mangalmurti

Xiong

Hulver

et al. 2009

Blood

105

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Section: Discussionsupporting

confidence: 92%

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

Mangalmurti

Xiong

Hulver

et al. 2009

Blood

105

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“…During storage, a progressive deterioration in the cellular, membranous, and metabolic properties of erythrocytes have been shown to take place, resulting in cell swelling, hemolysis, loss of deformability, and shape changes [1,2]. Shedding of membrane fragments as vesicles [3], and the inhibition in the cold of the Na + / K + -ATPase, with the consequent increase in intracellular Na + and decrease in intracellular K + contents [4], also occur. The concentrations of metabolites such as glucose, 2,3-BPG, and ATP decline with storage, and lactate accumulates, determining a pH decrease from 7.0 to *6.5 after 6 weeks of storage [5,6].…”

Section: Introductionmentioning

confidence: 99%

Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

Arduini¹,

Minetti

Ciana

et al. 2006

American J Hematol

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L-Carnitine (LC) in the preservation medium during storage of red blood cells (RBC) can improve the mean 24-hr percent recovery in vivo and increase RBC life-span after reinfusion. The purpose of the study was to investigate the differences in the biochemical properties of RBCs stored in the presence or absence of LC, and the cell-age related responses to storage conditions and to LC. RBC concentrates in saline-adenine-glucose-mannitol (SAG-M) were stored in the presence or absence of 5 mM LC at 48C for up to 8 weeks. RBC subpopulations of different densities were prepared by centrifugation on Stractan density gradient. Cells were sampled at 0, 3, 6, and 8 weeks, and hematological and cellular properties analyzed (MCV, MCHC, 4.1a/4.1b ratio as a cell age parameter, intracellular Na + and K + ). After 6 weeks, MCV of RBC stored in the presence of LC was lower than that of controls (6 weeks MCV: controls 95.4 ± 1.8 fl; LC 91.5 ± 2.0 fl; n = 6; P < 0.005). This was due to swelling of control cells, and affected mainly older RBCs. LC appeared to reduce or retard cell swelling. Among the osmotically active substances whose changes during storage could contribute to cell swelling, only intracellular Na

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“…During storage, ATP decreases in time leading to energetic compromise, loss of membrane stability and morphological and rheological changes including RBC adhesion to the endothelium. [8][9][10][11][12] …”

Section: Introductionmentioning

confidence: 99%

To Study the Morphological Changes Seen in Stored Blood in a Blood Bank

Gupta¹,

Goyal²,

Pantola³

et al. 2016

jemds

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BACKGROUNDThe viability and functionality of stored human blood cells is an important clinical issue in transfusion. 1 During storage, in fact preserved blood cells undergo progressive structural and functional changes that may reduce red cell function and viability a fter transfusion. 2 Storage has a negative effect on RBC oxygen delivery 3 and emerging evidence suggests that allogenic RBC infusion may actually harm some recipients. Considerable evidence suggests that transfusion increases the risk of serious complications and death in critically ill patients, especially in patients who are undergoing cardiac surgery. Current research indicated that the RBC storage lesion is responsible for impaired tissue oxygen use, pro-inflammatory and immunomodulatory effects, increased infections, multiple organ system failure and ultimately increased morbidity and mortality. 4 The aim of present study was to evaluate the morphological changes in blood cells due to processing and storage at interval of 1 day, 1 week, 2 weeks, 3 weeks, 4 weeks and 5 weeks and compare with the morphology at the time of collection (day 0).

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Erythrocyte Membrane Vesiculation and Changes in Membrane Composition during Storage in Citrate-Phosphate-Dextrose-Adenine-1

Cited by 37 publications

References 30 publications

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

To Study the Morphological Changes Seen in Stored Blood in a Blood Bank

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