Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes

Jain, Sushil K.; McVie, Robert; Duett, John; Herbst, John J.

doi:10.2337/diab.38.12.1539

Cited by 431 publications

(192 citation statements)

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“…This could be due to the fact that in an red blood cell study, the actual concentrations of three kinds of curcuminods entering the cell may be much lower and differences in the kinetics for glucose metabolism and lipid peroxidation or to a lack of sensitivity of the lipid peroxidation assay in comparison to the glucose assay [36]. Previous publication suggested this point that high concentration curcumin triggered a certain degree of eryptosis [35].…”

Section: Discussionmentioning

confidence: 98%

Effects of Three Kinds of Curcuminoids on Anti-Oxidative System and Membrane Deformation of Human Peripheral Blood Erythrocytes in High Glucose Levels

Yang

et al. 2015

Cell Physiol Biochem

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Background/Aims: Curcuminoids are the main bioactive constituents of the rhizome of turmeric. Erythrocytes lesions in diabetes are probably related to hyperglycemia and protein glycation. It has been reported that curcumin prevent lipid peroxidation. However, reports on the effects of demethoxycurcumin and bis-demethoxycurcumin on human erythrocytes at high glucose levels are scarce. Our aim is to investigate the effect of curcuminoids on oxidative stress and membrane of erythrocytes exposed to hyperglycemic condition. Methods: In this study, the different blood samples were treated with two doses of glucose (10 or 30 mM) to mimic hyperglycemia in the presence or absence of three kinds of curcuminoids (5 or 10 μM) in a medium at 37 °C for 24 h (Each experiment consists of 20 blood samples from 10 male and 10 female volunteers). The malondialdehyde was checked by HPLC, antioxidase (GSH and GSSG) were measured by LC/MS, SOD was checked by WST-1 kit, morphology and phospholipid symmetry were detected by flow cytometry, confocal scanning microscope and scanning electron microscope. Results: The results illustrated that all three curcuminoids reduce oxidative stress damage on the membrane and maintain a better profile for erythrocytes. Furthermore, three curcuminoids had benefit effects on antioxidase. Conclusion: The three kinds of curcuminoids supplementation may prevent lipid peroxidation at different intensity and membrane dysfunction of human erythrocytes in hyperglycemia.

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Section: Discussionmentioning

confidence: 98%

Effects of Three Kinds of Curcuminoids on Anti-Oxidative System and Membrane Deformation of Human Peripheral Blood Erythrocytes in High Glucose Levels

Yang

et al. 2015

Cell Physiol Biochem

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“…Packed red cells (0.2 ml) were used for the estimation of MDA as TBARS (18). The absorbance at 600 nm was subtracted from absorbance at 532 nm.…”

Section: Methodsmentioning

confidence: 99%

Housing in Pyramid Counteracts Neuroendocrine and Oxidative Stress Caused by Chronic Restraint in Rats

Bhat

Rao

Murthy

et al. 2006

Evidence-Based Complementary and Alternative Medicine

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The space within the great pyramid and its smaller replicas is believed to have an antistress effect. Research has shown that the energy field within the pyramid can protect the hippocampal neurons of mice from stress-induced atrophy and also reduce neuroendocrine stress, oxidative stress and increase antioxidant defence in rats. In this study, we have, for the first time, attempted to study the antistress effects of pyramid exposure on the status of cortisol level, oxidative damage and antioxidant status in rats during chronic restraint stress. Adult female Wistar rats were divided into four groups as follows: normal controls (NC) housed in home cage and left in the laboratory; restrained rats (with three subgroups) subject to chronic restraint stress by placing in a wire mesh restrainer for 6 h per day for 14 days, the restrained controls (RC) having their restrainers kept in the laboratory; restrained pyramid rats (RP) being kept in the pyramid; and restrained square box rats (RS) in the square box during the period of restraint stress everyday. Erythrocyte malondialdehyde (MDA) and plasma cortisol levels were significantly increased and erythrocyte-reduced glutathione (GSH) levels, erythrocyte glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were significantly decreased in RC and RS rats as compared to NC. However, these parameters were maintained to near normal levels in RP rats which showed significantly decreased erythrocyte MDA and plasma cortisol and significantly increased erythrocyte GSH levels, erythrocyte GSH-Px and SOD activities when compared with RS rats. The results showed that housing in pyramid counteracts neuroendocrine and oxidative stress caused by chronic restraint in rats.

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“…Malondialdehyde (MDA) generation in samples of plasma, jejunum and ileum was measured according to x Values for nutritional composition were calculated according to Sauvant et al (2004) the method of Jain et al (1989) as an index of lipid peroxidation and oxidative status (Michel et al 2008). Phosphate-buffered saline (800 mL, pH 7.4) and butylated hydroxytoluene solution (25 mL, 0.88%) were added to 200 mL of plasma and mixed thoroughly.…”

Section: Biochemical Analysismentioning

confidence: 99%

Antioxidant status and inflammatory response in weanling piglets fed diets supplemented with arginine and zinc

2014

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2014. Antioxidant status and inflammatory response in weanling piglets fed diets supplemented with arginine and zinc. Can. J. Anim. Sci. 94: 87Á97. Dietary supplementation with zinc oxide (Zn oxide) and arginine (Arg) is known to improve growth in weanling piglets. The mechanism of action is not yet well understood, although antioxidant effects and inflammatory responses may be involved. This experiment was conducted to evaluate the effects of Zn and Arg supplementation on the antioxidant status and inflammatory response of piglets. Thirty-two 20-dold weanling piglets were placed for 12 d on diets supplemented or not with Zn (2500 mg Zn oxide kg (1 ) and Arg (1%), designated ZN0ARG0, ZN2500ARG0, ZN0ARG1 and ZN2500ARG1. On day 12, blood samples were taken before and 3 h after intra-peritoneal injection of lipopolysaccharide (LPS; 10 mg kg (1 ). The piglets were euthanized just after the second blood sample, and samples of mucosae were taken from the jejunum and ileum for determination of mRNA expression and morphological observation. Zn supplementation decreased plasma malondialdehyde measured before LPS injection (PB0.05). Arg supplementation increased the ferric-reducing ability of plasma (indicator of antioxidant status) measured after LPS injection (PB0.05). Piglets fed Zn-supplemented diets had lower total plasma superoxide-dismutase (SOD) activity (P B0.05) but higher plasma tumour necrosis factor-a (P B0.05) after LPS injection. Zinc-supplemented diets increased metallothionein-1 expression and total antioxidant capacity in the ileum and jejunum (PB0.05) and decreased interleukin-10 expression (PB0.05) in the ileum. In the jejunum, the combination of Zn and Arg supplementation increased villus height (Arg)Zn, PB0.05). These results indicate that Zn may reduce systemic oxidation and improve the antioxidant status in the jejunal and ileal mucosae. However, Zn and Arg supplementation did not appear to act synergistically to enhance antioxidant status or reduce inflammation in weanling piglets.Abbreviations: ADFI, average daily feed intake; ADG, average daily weight gain; Arg, arginine; BW, body weight; FRAP, ferricreducing ability of plasma; G:F, growth-to-feed ratio; IL10, interleukin-10; IL12B, interleukin-12B; LPS, lipopolysaccharide; MDA, malondialdehyde; MT1, metallothionein-1; NOS2, nitric oxide synthase; NOx, plasma nitrite'nitrate concentration; PGE2, prostaglandin E2 metabolite; SOD, superoxide-dismutase; TAC, total antioxidant capacity; TGFB2, transforming growth factor-b 2 ; TNF, tumour necrosis factor-a Can.

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Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes

Cited by 431 publications

References 0 publications

Effects of Three Kinds of Curcuminoids on Anti-Oxidative System and Membrane Deformation of Human Peripheral Blood Erythrocytes in High Glucose Levels

Effects of Three Kinds of Curcuminoids on Anti-Oxidative System and Membrane Deformation of Human Peripheral Blood Erythrocytes in High Glucose Levels

Housing in Pyramid Counteracts Neuroendocrine and Oxidative Stress Caused by Chronic Restraint in Rats

Antioxidant status and inflammatory response in weanling piglets fed diets supplemented with arginine and zinc

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