Erythrocyte Membrane Fluidity Decreased in Uremic Hemodialyzed Patients

Komidori, Kazuo; Kamada, Takenobu; Yamashita, Tomoko; Harada, Ryuji; Otsuji, Yoshihito; Hashimoto, Shuji; Chuman, Yasuo; Otsuji, S

doi:10.1159/000183458

Cited by 21 publications

(6 citation statements)

References 9 publications

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“…Some plasma factors accumulating during the uremic state [6], elevated neuraminidase activity in serum [31], changed erythrocyte membrane fluidity [32], impair ment of Na-K pump in the erythrocyte [33] and increased purine content and abnormal pyrimidine nucleotides in uremic hemolysates [7] could all contribute to hemolysis in CRF.…”

Section: Discussionmentioning

confidence: 99%

Reduced Erythrocyte Defense Mechanisms against Free Radical Toxicity in Patients with Chronic Renal Failure

Durak¹,

Akyol²,

Başeşme

et al. 1994

Nephron

103

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In this study, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) enzymes were determined in the erythrocytes from patients with chronic renal failure (CRF) and from healthy subjects. In the conservative drug management group and intermittent ambulatory peritoneal dialysis group, CAT activity was lower than in the control group. However, SOD and GSH-Px activities of these groups were not statistically different from the control values. In the continuous ambulatory peritoneal dialysis group and the hemodialysis group, SOD, GSH-Px and CAT activities were lower than control values. In the patient groups, correlation coefficients between the enzyme activities were also found to be different from the control values. Results suggested that enzymatic antioxidant defense mechanisms were suppressed in the erythrocytes from the patients with CRF, in particular in the erythrocytes from those who were under hemodialysis and continuous ambulatory peritoneal dialysis management. It is proposed that reduced antioxidant defense mechanisms in the erythrocytes is one of the important factors leading to peroxidation in the membrane lipid structure of the erythrocytes and thereby to hemolysis and anemia in the patients with CRF.

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Section: Discussionmentioning

confidence: 99%

Reduced Erythrocyte Defense Mechanisms against Free Radical Toxicity in Patients with Chronic Renal Failure

Durak¹,

Akyol²,

Başeşme

et al. 1994

Nephron

103

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“…[45][46][47] In this context, an altered FA composition with reduced levels of PUFAs and increased contents of saturated FA (SFA), that consequently decrease the PUFA/SFA ratio in erythrocyte membranes, may be associated with lower membrane fluidity in patients with chronic diseases. 48,49 This is often found in elite athletes. [50][51][52] It was previously established that erythrocytes reflect the general FA metabolism in other organs and tissues.…”

Section: Pufas Intake In Relation To Status Biomarkermentioning

confidence: 98%

Polyunsaturated fatty acids in health and disease

et al. 2013

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Polyunsaturated fatty acids (PUFAs) are necessary for overall health. The two PUFAs families, n-6 and n-3 fatty acid are physiologically and metabolically distinct. Proportion of PUFAs in serum and erythrocyte phospholipids is an important determinant of both health and disease, and depends on endogenous metabolism controlled by genetic polymorphisms and dietary intake. Both n-3 and n-6 PUFAs are processed to powerful promoters of eicosanoids synthesis at the cyclooxygenase and lipoxygenase level. Evidence from observational and intervention studies suggest that n-3 PUFAs are cardioprotective, perhaps through their anti-inflammatory, anti-arrhythmic, lipid-lowering, and antihypertensive effects. In contrast, dietary n-6 PUFAs have pro-inflammatory effect. Low n-3 and elevated n-6 PUFAs levels were found in patients with cancer on different sites. The present review will focus on the current knowledge related to PUFAs intake and status in health and disease, with reference to the Serbian population. [Projekat Ministarstva nauke Republike Srbije, br. III41030

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“…Inhibitor substances accumulating in uremia [31], increased serum neuraminidase activity [37], changed erythrocyte membrane fluidity [38], impaired Na-K pump system in the erythrocytes [39] and increased purine/pyrimidine content in uremic hemolysates [40] have all been suggested to contribute to the hemolysis in patients with CRF. In addition to reduced antioxidant potential, artifacts occurring during dialysis, several uremic metabolites [31], oxidative stress due to hexose monophosphate shunt inhibition [23,24] and reduced ATP/ADP ratio due to oxidative metabolism [40] may all contribute to the increased toxic-free radical production in the erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Oxidant/Antioxidant Status of Erythrocytes from Patients with Chronic Renal Failure: Effects of Hemodialysis

Durak¹,

Kavutçu²,

Çimen³

et al. 2001

Med Princ Pract

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Objective: It has been suggested that oxidative processes may be increased in patients with chronic renal failure (CRF), and that this is a possible factor contributing to the development of anemia and atherosclerosis, characteristic complications of CRF. The aim of this study was to investigate erythrocyte oxidant/antioxidant status in patients with CRF and to elucidate possible effects of hemodialysis on erythrocyte antioxidant system. Methods: Fasting blood samples were obtained from 33 patients with CRF and from 12 healthy controls. Of the patients, 17 subjects were under regular hemodialysis. Values of the activities of antioxidant enzymes, namely superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and antioxidant potential, nonenzymatic superoxide radical scavenger activity (NSSA) and levels of thiobarbituric acid reagent substances (TBARS) were measured in the erythrocytes from both patients and controls. Results: Antioxidant potential and NSSA values were found to be significantly decreased, while TBARS levels were increased in the erythrocytes of patients. SOD activity was found to be unchanged, but GSH-Px and CAT activities were significantly lower in the patient group. Moreover, the erythrocyte TBARS level in the hemodialysis group was higher than in the controls and nonhemodialysis patients. Conclusion: The results suggest that antioxidant potential is reduced due to impaired antioxidant system in erythrocytes from patients with CRF and that oxidant stress causes significant peroxidation. Hemodialysis is determined to further increase oxidative reactions. These changes seem to contribute to the occurrence of some complications of CRF. Therefore, it has been suggested that antioxidant supplementation may give beneficial results for these patients.

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Erythrocyte Membrane Fluidity Decreased in Uremic Hemodialyzed Patients

Cited by 21 publications

References 9 publications

Reduced Erythrocyte Defense Mechanisms against Free Radical Toxicity in Patients with Chronic Renal Failure

Reduced Erythrocyte Defense Mechanisms against Free Radical Toxicity in Patients with Chronic Renal Failure

Polyunsaturated fatty acids in health and disease

Oxidant/Antioxidant Status of Erythrocytes from Patients with Chronic Renal Failure: Effects of Hemodialysis

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