Erythrocyte membrane fluidity as a marker of diabetic retinopathy in type 1 diabetes mellitus

Bianchetti, Giada; Viti, Luca; Scupola, Andrea; Leo, Mauro Di; Tartaglione, Linda; Flex, Andrea; Spirito, M.; Pitocco, Dario; Maulucci, Giuseppe

doi:10.1111/eci.13455

Cited by 18 publications

(17 citation statements)

References 24 publications

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“…NADH autofluorescence, excited at 740 nm, was acquired in the spectral emission range 425–475 nm. Contributions from cytoplasm and mitochondria, respectively, have been obtained separately by applying a machine learning-based pixel classification workflow through the open-source software Ilastik ( , accessed on 5 May 2022) [ 35 , 36 , 37 , 38 ]. The separation of the cytoplasmic and mitochondrial NAD(P)H responses through microscopy [ 39 ], perfectioned through time by optimizing acquisition and segmentation techniques, is nowadays a strong and reliable assessment of NAD(P)H redox state.…”

Section: Methodsmentioning

confidence: 99%

Investigation of DHA-Induced Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells through the Combination of Metabolic Imaging and Molecular Biology

Bianchetti

Clementi²,

Sampaolese³

et al. 2022

Antioxidants

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Diabetes-induced oxidative stress leads to the onset of vascular complications, which are major causes of disability and death in diabetic patients. Among these, diabetic retinopathy (DR) often arises from functional alterations of the blood-retinal barrier (BRB) due to damaging oxidative stress reactions in lipids, proteins, and DNA. This study aimed to investigate the impact of the ω3-polyunsaturated docosahexaenoic acid (DHA) on the regulation of redox homeostasis in the human retinal pigment epithelial (RPE) cell line (ARPE-19) under hyperglycemic-like conditions. The present results show that the treatment with DHA under high-glucose conditions activated erythroid 2-related factor Nrf2, which orchestrates the activation of cellular antioxidant pathways and ultimately inhibits apoptosis. This process was accompanied by a marked increase in the expression of NADH (Nicotinamide Adenine Dinucleotide plus Hydrogen) Quinone Oxidoreductase 1 (Nqo1), which is correlated with a contextual modulation and intracellular re-organization of the NAD+/NADH redox balance. This investigation of the mechanisms underlying the impairment induced by high levels of glucose on redox homeostasis of the BRB and the subsequent recovery provided by DHA provides both a powerful indicator for the detection of RPE cell impairment as well as a potential metabolic therapeutic target for the early intervention in its treatment.

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Section: Methodsmentioning

confidence: 99%

Investigation of DHA-Induced Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells through the Combination of Metabolic Imaging and Molecular Biology

Bianchetti

Clementi²,

Sampaolese³

et al. 2022

Antioxidants

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“…Cordelli's study [ 40 ] found that erythrocyte liquidity could be used as an auxiliary indicator to analyze the progression of diabetes. Bianchetti et al [ 41 ] reported that the reduced erythrocyte membrane fluidity caused by increasing nonenzymatic glycosylation, reactive oxygen species, and lipid peroxidation may be an indicator of retinopathy of T1DM. The fluidity change of diabetic erythrocytes increases their aggregation and weakens their deformability and further leads to their metabolic disorders.…”

Section: Erythrocyte Changes In Patients With Diabetesmentioning

confidence: 99%

“…Therefore, HbA1c is an effective indicator for preventing and monitoring diabetic complication. When combined with other erythrocyte indicators such as membrane fluidity, it can monitor DR better [ 41 ].…”

Section: Erythrocyte Changes In Patients With Diabetesmentioning

confidence: 99%

The Relationship between Erythrocytes and Diabetes Mellitus

Wang

Yang

Zhou

et al. 2021

Journal of Diabetes Research

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High blood glucose level (hyperglycemia) is a leading indicator of diabetes mellitus (DM). Erythrocytes are the most abundant cells in the circulation and the first to perceive changes in plasma composition. Long-lasting hyperglycemia affects the structure and function of erythrocytes. The detection of erythrocyte-related indicators can provide a valuable reference for the prevention, diagnosis, and treatment of DM and its complications. This paper reviews the normal structure and function of erythrocytes, the changes in erythrocytes in patients with diabetes, and the role of erythrocytes in the development of diabetic complications to provide more indicators for the early prevention of DM complications and to monitor the therapeutic effect of DM.

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“…These processes are associated with molecular motion within a lipid bilayer and activity regulation of other molecules, including membrane-bound proteins. It was reported that abnormalities in the physical properties of cell membranes may underlie the defects that are strongly linked to hypertension, stroke and other cardiovascular diseases [ 7 , 8 ], diabetes mellitus [ 9 ], and Alzheimers [ 10 ]. Therefore, studies of the relationships between fluidity and phase transitions in membranes are relevant.…”

Section: Introductionmentioning

confidence: 99%

Infrared Spectroscopic Study of Multi-Component Lipid Systems: A Closer Approximation to Biological Membrane Fluidity

Klaiss-Luna

Manrique-Moreno

2022

Membranes

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Membranes are essential to cellular organisms, and play several roles in cellular protection as well as in the control and transport of nutrients. One of the most critical membrane properties is fluidity, which has been extensively studied, using mainly single component systems. In this study, we used Fourier transform infrared spectroscopy to evaluate the thermal behavior of multi-component supported lipid bilayers that mimic the membrane composition of tumoral and non-tumoral cell membranes, as well as microorganisms such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. The results showed that, for tumoral and non-tumoral membrane models, the presence of cholesterol induced a loss of cooperativity of the transition. However, in the absence of cholesterol, the transitions of the multi-component lipid systems had sigmoidal curves where the gel and fluid phases are evident and where main transition temperatures were possible to determine. Additionally, the possibility of designing multi-component lipid systems showed the potential to obtain several microorganism models, including changes in the cardiolipin content associated with the resistance mechanism in Staphylococcus aureus. Finally, the potential use of multi-component lipid systems in the determination of the conformational change of the antimicrobial peptide LL-37 was studied. The results showed that LL-37 underwent a conformational change when interacting with Staphylococcus aureus models, instead of with the erythrocyte membrane model. The results showed the versatile applications of multi-component lipid systems studied by Fourier transform infrared spectroscopy.

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Erythrocyte membrane fluidity as a marker of diabetic retinopathy in type 1 diabetes mellitus

Cited by 18 publications

References 24 publications

Investigation of DHA-Induced Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells through the Combination of Metabolic Imaging and Molecular Biology

Investigation of DHA-Induced Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells through the Combination of Metabolic Imaging and Molecular Biology

The Relationship between Erythrocytes and Diabetes Mellitus

Infrared Spectroscopic Study of Multi-Component Lipid Systems: A Closer Approximation to Biological Membrane Fluidity

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