Erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy

Abstract

Recently, RBC membrane coated nanoparticles have attracted much attention because of their excellent immune escape ability; meanwhile, Au nanocages (AuNs) have been extensively used for cancer therapy due to its photothermal effect and drug delivery capability. The combination of RBC membrane coating and Au nanocages may provide an effective approach for targeted cancer therapy. However, few reports have shown the utilization of combining these two technologies. Here, we present the development of Erythrocyte membrane-coated Gold nanocages for targeted cancer photothermal and chemical therapy. First, anti-EpCam antibodies are used to modify RBC membranes to target 4T1 cancer cells. Second, the antitumor drug paclitaxel is encapsulated into AuNs. Then, the AuNs are coated with the modified RBC membranes. This new nanoparticles are termed EpCam-RPAuNs. We characterize the capability of EpCam-RPAuNs for selective tumor targeting via exposure to the near-infrared irradiation. Experimental results demonstrate that EpCam-RPAuNs can effectively generate hyperthermia and precisely deliver the antitumor drug PTX to targeted cells. We also validate the biocompatibility of our EpCam-RPAuNs in vitro. By combining the targeting moleculars modified RBC membrane and AuNs, our approach provides a new way to design biomimetic nanoparticles to enhance the surface functionality of nanoparticles. We believe that EpCam-RPAuNs can be potentially applied for cancer diagnoses and therapies.

Red blood cell membrane-camouflaged nanoparticles: a novel drug delivery system for antitumor application

“…, 137. Triphenylphosphonium (TPP) Targeting to mitochondria and enhancing the yield of 1 O 2 106 rHuPH20 Assisting nanoparticle diffusion in the cytoplasmic HA matrix of PC3 cells 64 Aptamer AS1411 Showing targeting capability against several cancer cell types 136 CDx peptides Ability to traverse the blood–brain barrier and achieve marked therapeutic efficacy in glioma treatment 138 Mannose Actively targeting APCs (antigen presenting cells) in lymphatic organs 97 Anti-EpCam (epithelial cell adhesion molecule) antibodies Exhibiting better targeting capacity of 4T1 cells compared to unmodified nanoparticles 139 RGD (Arg–Gly–Asp) Realizing stronger tumor growth inhibition effects than typical tumor-targeting peptides 140 …”

Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

“…Over the last two decades, Au NPs have been mainly used as biosensors and as contrast agents in X-ray imaging and computed tomography (Kobayashi et al 2016 ), being applied in biological and biomedical research, especially in cancer diagnosis (Chuang et al 2018 ; Kim et al 2018 ; Sugumaran et al 2018 ) and therapy (Khandanlou et al 2018 ; Zhu et al 2018 ), biosensing (Baetsen-Young et al 2018 ; Chen et al 2009 ), virus detection, and regulation of cell function (Bodelon et al 2017 ; Giljohann et al 2010 ).…”

Cell Membrane-Camouflaged Nanocarriers for Cancer Diagnostic and Therapeutic

“…In another study, RGD peptide [c(RGDyC)]modified platelet vesicles were employed to co-load melanin nanoparticles (MNPs) and DOX to achieve chemo-photothermal therapy for drug-resistant tumors (Jing et al, 2018). In addition, numerous CM-coated NPs have been developed for cancer photothermal-chemotherapy including RBC-melanoma cell hybrid membrane-coated, DOX-loaded hollow copper sulfide nanoparticles (Wang et al, 2018); RBC-camouflaged, PTX-loaded polymeric NPs (Su et al, 2016); RBCmimetic hollow mesoporous PB NPs (Chen et al, 2017a); and RBC-coated, PTX-loaded gold nanocages (Zhu et al, 2018). Furthermore, synergistic treatment using PTT and immunotherapy was investigated.…”