Erythrocyte Glutathione Transferase as a Biomarker in Kidney Health and Disease

Bocedi, Alessio; Noce, Annalisa; Fabrini, Raffaele; Daniele, Nicola Di; Galli, Francesco; Ricci, Giorgio

doi:10.1007/978-94-007-7699-9_44

Cited by 4 publications

(1 citation statement)

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“…These findings align with and expand upon prior studies, which have shown elevation of these proteins in different bodily fluids. [52][53][54] Upregulation of oxidative stress mediators as well as glycolysis proteins in patients with CKD may provide additional insight into the molecular changes that occur within the early stage of the disease.…”

Section: Renal Function and Urinary Proteomementioning

confidence: 99%

Urine Proteomics Reveals Sex-Specific Response to Total Pancreatectomy With Islet Autotransplantation

et al. 2022

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Objectives: Total pancreatectomy with islet autotransplantation (TPIAT) is a surgical option for refractory chronic pancreatitis-related pain. Despite the known clinical implications of TPIAT, the molecular effects remain poorly investigated. We performed the first hypothesis-generating study of the urinary proteome before and after TPIAT.Methods: Twenty-two patients eligible for TPIAT were prospectively enrolled. Urine samples were collected the week before and 12 to 18 months after TPIAT. The urine samples were prepared for bottom-up label-free quantitative proteomics using the "MStern" protocol.Results: Using 17 paired samples, we identified 2477 urinary proteins, of which 301 were significantly changed post-TPIAT versus pre-TPIAT. Our quantitative analysis revealed that the molecular response to TPIAT was highly sex-specific, with pronounced sex differences pre-TPIAT but minimal differences afterward. Comparing post-TPIAT versus pre-TPIAT, we found changes in cell-cell adhesion, intracellular vacuoles, and immune response proteins. After surgery, immunoglobulins, complement proteins, and cathepsins were increased, findings that may reflect glomerular damage. Finally, we identified both known and novel markers for immunoglobulin A nephropathy after 1 patient developed the disease 2 years after TPIAT. Conclusions:We found distinct changes in the urinary proteomic profile after TPIAT and the response to TPIAT is highly sex-specific.

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Section: Renal Function and Urinary Proteomementioning

confidence: 99%