Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay

Novembrino, Cristina; Porcella, Antonella; Conte, Dario; Vecchi, Amedeo F. De; Buccianti, G.; Lonati, Silvia; Duca, Lorena; Ciani, A.; Bamonti-Catena, F.

doi:10.1515/cclm.2005.079

Cited by 10 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, importantly, ferritin is made in cells (including intestinal cells), and not in serum. We also note the evidence for the presence of ferritin within erythrocytes, [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] the largest volume fraction of serum. 55 In nucleated cells, ferritin resides mainly in the cytoplasm, but there are nuclear [56][57][58][59][60][61] and mitochondrial [62][63][64] forms (not considered here, as our focus is serum ferritin).…”

Section: A Systems Biology Overview Of Human Iron Metabolismmentioning

confidence: 99%

Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells

2014

View full text Add to dashboard Cite

"Serum ferritin" presents a paradox, as the iron storage protein ferritin is not synthesised in serum yet is to be found there. Serum ferritin is also a well known inflammatory marker, but it is unclear whether serum ferritin reflects or causes inflammation, or whether it is involved in an inflammatory cycle. We argue here that serum ferritin arises from damaged cells, and is thus a marker of cellular damage. The protein in serum ferritin is considered benign, but it has lost (i.e. dumped) most of its normal complement of iron which when unliganded is highly toxic. The facts that serum ferritin levels can correlate with both disease and with body iron stores are thus expected on simple chemical kinetic grounds. Serum ferritin levels also correlate with other phenotypic readouts such as erythrocyte morphology. Overall, this systems approach serves to explain a number of apparent paradoxes of serum ferritin, including (i) why it correlates with biomarkers of cell damage, (ii) why it correlates with biomarkers of hydroxyl radical formation (and oxidative stress) and (iii) therefore why it correlates with the presence and/or severity of numerous diseases. This leads to suggestions for how one might exploit the corollaries of the recognition that serum ferritin levels mainly represent a consequence of cell stress and damage.

show abstract

Section: A Systems Biology Overview Of Human Iron Metabolismmentioning

confidence: 99%

Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells

2014

View full text Add to dashboard Cite

show abstract

“…Both types can be measured following total lysis of the erythrocytes and after thorough removal of both plasma and contaminating WBCs, which contain approximately 1000 times more ferritin than erythrocytes. While this assay could be run on automated analyzers using the regular serum ferritin methodology 165 , it is infrequently used as the process requires complete removal of WBCs to avoid measuring leukocyte ferritin 166 , and erythrocyte ferritin does not reflect dynamic changes in iron status; thus, it is rarely available to clinicians.…”

Section: Erythrocyte Ferritinmentioning

confidence: 99%

Diagnosis of iron-deficient states

Archer

Brugnara

2015

Critical Reviews in Clinical Laboratory Sciences

View full text Add to dashboard Cite

The diagnosis of iron deficiency anemia is typically straightforward, especially when classic biochemical and hematological changes are present in a subject at risk. It can be challenging in the presence of diseases or when it is due to inherited defects of iron metabolism. The identification of iron deficiency prior to anemia development is also difficult. New hematological parameters such as reticulocyte Hb content have expanded the classic ones such as MCV, MCH and MCHC. A variety of hematology analyzers now provide novel parameters to assess cellular hypochromia and microcytosis in both reticulocytes and mature red blood cells. The repertoire of biochemical markers has also been expanded, with iron, transferrin and ferritin being supplemented by circulating transferrin receptor and hepcidin. Molecular identification of functional variants of key iron metabolism determinants has provided explanations for the heritability of some iron metabolism biomarkers. Genetic defects in some of these molecules are responsible for hereditary microcytic anemias, also called atypical microcytic anemias. In this review, we examine the most significant hematological and biochemical markers for iron metabolism, as well as relevant genetic polymorphisms and defects affecting iron handling.

show abstract

“…Eleven thousand eight hundred ninety three records were obtained from the search strategy depicted in Table 1 . From 252 records extracted, 187 studies were included in the qualitative analysis [ 8 , 10 , 15 – 199 ] and 148 in the meta-analysis [ 8 , 10 , 15 , 17 , 18 , 22 , 23 , 26 , 28 , 30 – 32 , 34 – 56 , 58 – 64 , 66 – 68 , 72 , 73 , 75 , 77 – 80 , 82 – 85 , 87 – 92 , 94 – 98 , 100 – 102 , 106 – 109 , 111 – 116 , 120 – 137 , 139 – 143 , 145 , 146 , 148 – 151 , 153 – 157 , 159 – 164 , 166 – 175 , 177 , 179 – ...…”

Section: Resultsmentioning

confidence: 99%

Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis

et al. 2018

View full text Add to dashboard Cite

BackgroundDifferent laboratory methods are used to quantify ferritin concentrations as a marker of iron status. A systematic review was undertaken to assess the accuracy and comparability of the most used methods for ferritin detection.Methods and findingsNational and regional databases were searched for prospective, retrospective, sectional, longitudinal and case-control studies containing the characteristics and performance of at least one method for serum/plasma ferritin determinations in humans published to date. The analysis included the comparison between at least 2 methods detailing: sensitivity, precision, accuracy, predictive values, inter-methods adjustment, and use of international reference materials. Pooled method performance was analyzed for each method and across methods.OutcomesSearch strategy identified 11893 records. After de-duplication and screening 252 studies were assessed, including 187 studies in the qualitative analysis and 148 in the meta-analysis. The most used methods included radiometric, nonradiometric and agglutination assays. The overall within-run imprecision for the most reported ferritin methods was 6.2±3.4% (CI 5.69–6.70%; n = 171), between-run imprecision 8.9±8.7% (CI 7.44–10.35%; n = 136), and recovery rate 95.6% (CI 91.5–99.7%; n = 94). The pooled regression coefficient was 0.985 among all methods analyzed, and 0.984 when comparing nonradiometric and radiometric methods, without statistical differences in ferritin concentration ranging from 2.3 to 1454 μμg/L.ConclusionThe laboratory methods most used to determine ferritin concentrations have comparable accuracy and performance. Registered in PROSPERO CRD42016036222.

show abstract

Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay

Cited by 10 publications

References 11 publications

Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells

Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells

Diagnosis of iron-deficient states

Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis

Contact Info

Product

Resources

About