Erythrocyte‐Derived Nanoparticles as a Theranostic Agent for Near‐Infrared Fluorescence Imaging and Thrombolysis of Blood Clots

Vankayala, Raviraj; Corber, Samantha R.; Mac, Jenny T.; Rao, Masaru P.; Shafie, Mohammad; Anvari, Bahman

doi:10.1002/mabi.201700379

Cited by 32 publications

(24 citation statements)

References 28 publications

(61 reference statements)

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“…Biomimetic NPs, mainly based on the use of cell membranes of both platelets and red blood cells, are invisible to the phagocytic system, and aim at increasing the half-life of the NPs and improving the targeting efficacy to the thrombus region. Promising results were obtained for the fibrinolytic activity with rtPA conjugated on the surface of red blood cell membranes [49]. One of the first successful studies using platelets to encapsulate rtPA was carried out by Hu et al, who published a biomimetic NP based on platelet membrane coating.…”

Section: Clot Targets For Rtpa Nanocarrier Vectorizationmentioning

confidence: 99%

New Approaches in Nanomedicine for Ischemic Stroke

Correa-Paz

Silva‐Candal²,

Polo

et al. 2021

Pharmaceutics

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Ischemic stroke, caused by the interruption of blood flow to the brain and subsequent neuronal death, represents one of the main causes of disability in developed countries. Therapeutic methods such as recanalization approaches, neuroprotective drugs, or recovery strategies have been widely developed to improve the patient’s outcome; however, important limitations such as a narrow therapeutic window, the ability to reach brain targets, or drug side effects constitute some of the main aspects that limit the clinical applicability of the current treatments. Nanotechnology has emerged as a promising tool to overcome many of these drug limitations and improve the efficacy of treatments for neurological diseases such as stroke. The use of nanoparticles as a contrast agent or as drug carriers to a specific target are some of the most common approaches developed in nanomedicine for stroke. Throughout this review, we have summarized our experience of using nanotechnology tools for the study of stroke and the search for novel therapies.

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Section: Clot Targets For Rtpa Nanocarrier Vectorizationmentioning

confidence: 99%

New Approaches in Nanomedicine for Ischemic Stroke

Correa-Paz

Silva‐Candal²,

Polo

et al. 2021

Pharmaceutics

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“…13,14,20 Recently, use of cells such as erythrocytes or nanoparticles coated with cell membranes as therapeutic or imaging platforms has drawn increased attention. [21][22][23][24][25][26][27][28][29][30][31] A particular feature of erythrocytes that distinguishes them from other cell types is their naturally long circulation time (≈90 to 120 days) attributed to the presence of "self-marker" proteins on their surface to inhibit the immune response. 32,33 One putative self-marker is CD47 glycoprotein, which impedes phagocytosis through signaling with phagocytes receptor, SIRPα.…”

Section: Introductionmentioning

confidence: 99%

“…39 We have previously demonstrated the effectiveness of antibody-functionalized NETs in targeted imaging of ovarian cancer cells, as well as NETs functionalized with tissue plasminogen activator as a theranostic agent for NIR fluorescence imaging and thrombolysis of blood clots in vitro. 23,24 Herein we report for the first time the engineering of NETs functionalized with the ligand binding domain (LBD) of the EphB1 receptor ( Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

et al. 2019

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Over-or under-expression of erythropoietin-production human hepatocellular receptors (Eph) and their ligands are associated with various diseases. Therefore, these molecular biomarkers can potentially be used as binding targets for the delivery of therapeutic and/or imaging agents to cells characterized by such irregular expressions. We have engineered nanoparticles derived from erythrocytes and doped with the near-infrared (NIR) FDA-approved dye, indocyanine green. We refer to these nanoparticles as NIR erythrocyte-derived transducers (NETs). We functionalized the NETs with the ligand-binding domain of a particular Eph receptor, EphB1, to target the genetically modified human dermal microvascular endothelial cells (hDMVECs) with coexpression of EphB1 receptor and its ligand ephrin-B2. This cell model mimics the pathological phenotypes of lesional endothelial cells (ECs) in port wine stains (PWSs). Our quantitative fluorescence imaging results demonstrate that such functionalized NETs bind to the ephrin-B2 ligands on these hDMVECs in a dose-dependent manner that varies sigmoidally with the number density of the particles. These nanoparticles may potentially serve as agents to target PWS lesional ECs and other diseases characterized with over-expression of Eph receptors or their associated ligands to mediate phototherapy.

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“…Recently, we have encapsulated ICG using hemoglobindepleted red blood cells (RBCs), also known as erythrocyte ghosts (EGs) [54][55][56][57]. We refer to these ICG loaded particles as NIR erythrocyte-mimicking transducers (NETs) since they can use NIR light to generate heat, fluorescence, and reactive oxygen species [58].…”

mentioning

confidence: 99%

“…We refer to these ICG loaded particles as NIR erythrocyte-mimicking transducers (NETs) since they can use NIR light to generate heat, fluorescence, and reactive oxygen species [58]. Several groups, including our own, have investigated NETs for imaging, phototherapeutic, and drugdelivery applications [55][56][57][59][60][61][62]. EGs have been used to encapsulate and deliver other therapeutic and diagnostic payloads as well [63][64][65].…”

mentioning

confidence: 99%

Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics

Tang

Partono

Anvari

2019

IEEE Trans. Biomed. Eng.

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Exogenous fluorescent materials activated by near-infrared (NIR) light can offer deep optical imaging with sub-cellular resolution, and enhanced image contrast. We have engineered NIR particles by doping hemoglobin-depleted erythrocyte ghosts (EGs) with indocyanine green (ICG). We refer to these optical particles as NIR erythrocyte-mimicking transducers (NETs). A particular feature of NETs is that their diameters can be tuned from micrometer to nanometer scale, thereby, providing a capability for broad NIR biomedical imaging applications. Herein, we investigate the effects of ICG concentration on key material properties of micrometer-sized NETs, and nanometer-sized NETs fabricated by either sonication or mechanical extrusion of EGs. The zeta potentials of NETs do not vary significantly with ICG concentration, suggesting that ICG is completely encapsulated within NETs regardless of particle size or ICG concentration. Loading efficiency of ICG into the NETs monotonically decreases with increasing values of ICG concentration. Based on quantitative analyses of the fluorescence emission spectra of the NETs, we determine that 20 μM ICG utilized during fabrication of NETs presents an optimal concentration that maximizes the integrated fluorescence emission for micrometer- and nanometer-sized NETs. Encapsulation of ICG in these constructs also enhanced the fluorescence stability and quantum yield of ICG. These results guide the engineering of NETs with maximal NIR emission for imaging applications such as fluorescence-guided tumor resection, and real-time angiography.

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Erythrocyte‐Derived Nanoparticles as a Theranostic Agent for Near‐Infrared Fluorescence Imaging and Thrombolysis of Blood Clots

Cited by 32 publications

References 28 publications

New Approaches in Nanomedicine for Ischemic Stroke

New Approaches in Nanomedicine for Ischemic Stroke

Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics

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