Erythema chronicum migrans of Lyme disease: Diagnosis by monoclonal antibodies

Park, H. Kim; Jones, Billy E.; Barbour, Alan G.

doi:10.1016/s0190-9622(86)70190-4

Cited by 45 publications

(17 citation statements)

References 21 publications

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“…29 Controversy remains over how B. burgdorferi induces clinicopathologic changes, but tissue damage is likely secondary to inflammation directed at persistent borrelial antigen. 36 We have not yet addressed the issue of whether B. burgdorferi organisms are present within the foci of inflammation in infected ponies.…”

Section: Discussionmentioning

confidence: 99%

Experimental Infection of Ponies with Borrelia burgdorferi by Exposure to Ixodid Ticks

et al. 2000

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Abstract. Seven specific-pathogen-free (SPF) ponies, 1-5 years old, were exposed to Borrelia burgdorferiinfected adult ticks while being treated with dexamethasone over 5 consecutive days. One SPF pony (pony No. 178) was first exposed to laboratory-reared nymphs without B. burgdorferi infection and 3 weeks later was exposed to B. burgdorferi-infected adult ticks with concurrent dexamethasone treatment for 5 consecutive days. Four uninfected ponies treated with dexamethasone, exposed to laboratory-reared ticks without B. burgdorferi infection served as uninfected controls. Clinical signs, bacteriologic culture, polymerase chain reaction (PCR) for bacterial DNA, immunologic responses, and gross lesions and histopathologic changes were investigated during the experiment or at necropsy 9 months after tick exposure. In all of the seven challenged ponies, infection with B. burgdorferi was detected from monthly skin biopsies and various tissues at postmortem examination by culture and by PCR. However, pony No. 178 exposed to laboratory-reared nymphs (without B. burgdorferi infection) and challenged with B. burgdorferi-infected adult ticks 2 months later did not develop a B. burgdorferi infection. All of the infected ponies seroconverted. Control ponies and pony No. 178 were negative by culture, PCR, and serology. Except for skin lesions, we failed to induce any significant histopathologic changes in this study. This is the first report of successful tick-induced experimental infection in ponies by exposure to B. burgdorferi-infected ticks. This Lyme disease model will be very useful to evaluate efficacy of vaccines against the Lyme agent and the effect of antibiotic therapy on horses infected with B. burgdorferi.

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Section: Discussionmentioning

confidence: 99%

Experimental Infection of Ponies with Borrelia burgdorferi by Exposure to Ixodid Ticks

et al. 2000

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“…In the second and third stages of Lyme borreliosis there may be skin, joint, nervous system, or cardiac involvement (10,133,142,164). The second-stage manifestations usually start a few weeks to a few months after the initial ECM.…”

Section: Clinical Aspectsmentioning

confidence: 99%

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

129

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Lyme borreliosis (Lyme disease), a common tick-borne disorder of people and domestic animals in North America and Europe, is caused by the spirochete Borrelia burgdorferi. Following the discovery and initial propagation of this agent in 1981 came revelations that other tick-associated infectious disorders are but different forms of Lyme borreliosis. A challenge for the clinician and microbiology laboratory is confirmation that a skin rash, a chronic meningitis, an episode of myocarditis, or an arthritic joint is the consequence of B. burgdorferi infection. The diagnosis of Lyme borreliosis may be established by (i) directly observing the spirochete in host fluid or tissue, (ii) recovering the etiologic spirochete from the patient in culture medium or indirectly through inoculation of laboratory animals, or (iii) carrying out serologic tests with the patient's serum or cerebrospinal fluid. The last method, while lacking in discriminatory power, is the most efficacious diagnostic assay for most laboratories at present.

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“…Such organisms seem readily demonstrable in sectioned skin, particularly when sampled from the advancing edge of the erythema migrans lesion (Kornblatt et al, 1984;Aberer et al, 1988;Park at al., 1986;Berger et al, 1983;Berger, B.W., 1984;De Koning J. and Hoogkamp-Korstanje J.A.A., 1986).…”

Section: Discussionmentioning

confidence: 99%

Determinants of Infectivity of Pathogens in Vector Ticks

Spielman¹

1990

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Erythema chronicum migrans of Lyme disease: Diagnosis by monoclonal antibodies

Cited by 45 publications

References 21 publications

Experimental Infection of Ponies with Borrelia burgdorferi by Exposure to Ixodid Ticks

Experimental Infection of Ponies with Borrelia burgdorferi by Exposure to Ixodid Ticks

Laboratory aspects of Lyme borreliosis

Determinants of Infectivity of Pathogens in Vector Ticks

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