Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

Abstract

A novel, Gram-stain positive, facultative anaerobic, non-motile and straight to curve rod shaped bacterium, strain LV19(T) was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus, which was collected from Yeong-dong, Chuncheongbuk-do, South Korea. The colonies of the new isolate were convex, circular, cream white in color and 1-2 mm in diameter after 3 days incubation on Tryptic Soy Agar at 37 °C. Based on the 16S rRNA gene sequence similarity, the new isolate was most closely related to Erysipelothrix inopinata MF-EP02(T), E. rhusiopathiae ATCC 19414 (T) and E. tonsillarum T-305(T) (94.8, 93.8 and 93.7 % similarity, respectively). Strain LV19(T) grew optimally at 37 °C, at pH 8.0 and in the presence of 0.5 % (w/v) NaCl. Oxidase activity and catalase activity were negative. The major cellular fatty acids (>10 %) were C18:2 cis-9,12 (28.9 %), C18:1 cis-9 (22.3 %), C16:0 (22.2 %) and C18:0 (18.5 %). The cell-wall hydrolysates contained ribose as a major sugar. Major polar lipids were phosphatidylglycerol and three unidentified glycolipids. No quinone was detected. The G+C content of the genomic DNA was 36.3 mol%. The levels of DNA-DNA relatedness between strain LV19(T) and all the reference strains were less than 20 %. On the basis of polyphasic evidence from this study, the isolate is considered to represent a novel species of the genus Erysipelothrix, for which the name Erysipelothrix larvae sp. nov. is proposed; the type strain is LV19(T) (=KCTC 33523(T) = DSM 28480(T)).

Whole genome sequencing of “Faecalibaculum rodentium” ALO17, isolated from C57BL/6J laboratory mouse feces

“…The isolate was Gram-stain positive, non-motile, non-spore forming small rod, oxidase and catalase negative. As previously described [ 13 , 15 ], strictly anaerobic techniques were used for the preparation of the DSM 104 medium ( http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104.pdf ) and the cultivation process (the gas atmosphere was 100 % N 2 ). Strain ALO17 was optimally cultivated in the DSM 104 broth at 37 °C and at pH 7 for 3 days under strict anaerobic condition.…”

Genomic analysis of the multi-host pathogen Erysipelothrix rhusiopathiae reveals extensive recombination as well as the existence of three generalist clades with wide geographic distribution

“…strain 2 [ 21 , 22 ], as well as the recently identified E. larvae sp. nov. [ 23 ]. E. rhusiopathiae and E. tonsillarum have long been recognized as distinct species based on differences in pathogenicity, phenotypic characteristics, and serotype groups [ 21 , 24 ], supported by DNA-DNA hybridization studies [ 25 ].…”

Whole-genome sequence of Clostridium lituseburense L74, isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus

“…A Gram-positive staining, rod-shaped, non-motile, spore-forming anaerobic bacterium, was isolated from the larval gut of a rhinoceros beetle, Trypoxylus dichotomus and characterized. Genomic DNA was purified from 2-L cultures of C. lituseburens L74 [4] . The extracted DNA sample was extracted using bead-beating technique [5] and sequenced on HiSeq 2000 (Illumina).…”