Eryptosis of non-parasitized erythrocytes is related to anemia in Plasmodium berghei low parasitema malaria of Wistar rats

Totino, Paulo Renato Rivas; Souza, Hugo Amorim dos Santos de; Correa, Edmar Henrique Costa; Daniel-Ribeiro, Cláudio Tadeu; Ferreira-da-Cruz, Maria de Fátima

doi:10.1007/s00436-018-6167-1

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…We concluded that these pathways are involved in the treatment of anaemia [98][99][100][101]. Interestingly, toxoplasmosis, malaria, African trypanosomiasis, hepatitis B and prostate cancer are all associated with anaemia [102][103][104][105][106], demonstrating that the progression of this disease is influenced by other diseases. To some extent, these results also indicate that PRA contains multiple active components and can achieve multiple objectives through multiple pathways.…”

Section: Discussionmentioning

confidence: 88%

Uncovering the mechanism of the effects of Paeoniae Radix Alba on iron-deficiency anaemia through a network pharmacology-based strategy

Deng

Ren

et al. 2020

BMC Complement Med Ther

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Background: Paeoniae Radix Alba, the root of the plant Paeonia lactiflora Pall, is a common blood-enriching drug in traditional Chinese medicine. Its effectiveness in the clinical treatment of anaemia is remarkable, but its potential pharmacologic mechanism has not been clarified. Methods: In this study, the potential pharmacologic mechanism of Paeoniae Radix Alba in the treatment of iron-deficiency anaemia was preliminarily elucidated through systematic and comprehensive network pharmacology. Results: Specifically, we obtained 15 candidate active ingredients from among 146 chemical components in Paeoniae Radix Alba. The ingredients were predicted to target 77 genes associated with iron-deficiency anaemia. In-depth analyses of these targets revealed that they were mostly associated with energy metabolism, cell proliferation, and stress responses, suggesting that Paeoniae Radix Alba helps alleviate irondeficiency anaemia by affecting these processes. In addition, we conducted a core target analysis and a cluster analysis of protein-protein interaction (PPI) networks. The results showed that four pathways, the p53 signalling pathway, the IL-17 signalling pathway, the TNF signalling pathway and the AGE-RAGE signalling pathway in diabetic complications, may be major pathways associated with the ameliorative effects of Paeoniae Radix Alba on iron-deficiency anaemia. Moreover, molecular docking verified the credibility of the network for molecular target prediction. Conclusions: Overall, this study predicted the functional ingredients in Paeoniae Radix Alba and their targets and uncovered the mechanism of action of this drug, providing new insights for advanced research on Paeoniae Radix Alba and other traditional Chinese medicines.

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Section: Discussionmentioning

confidence: 88%

Uncovering the mechanism of the effects of Paeoniae Radix Alba on iron-deficiency anaemia through a network pharmacology-based strategy

Deng

Ren

et al. 2020

BMC Complement Med Ther

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“…First, we can discuss eryptosis as a protective mechanism of the host against infection. This is because during Plasmodium infection, not only infected red blood cells undergo eryptosis, but also uninfected red blood cells, which become refractory to infection (41)(42)(43). We could say that this would be a mechanism of the host to contain the infection.…”

Section: Discussionmentioning

confidence: 99%

“…Eryptosis also appears to be induced in uninfected red blood cells (Figure 1), in infections by P. yoelii, P. berghei and by P. falciparum (41)(42)(43). Infected and uninfected red blood cells are likely to suffer eryptosis, the latter case being responsible for a worsening of the anemia caused by the infection.…”

Section: Pathological And/or Beneficial Roles Of Eryptosis In Malariamentioning

confidence: 99%

Eryptosis as a New Insight in Malaria Pathogenesis

2022

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Eryptosis is a programmed cell death-like process that occurs in red blood cells. Although the red blood cells are anucleated, there are similarities between eryptosis and apoptosis, such as increased calcium efflux, calpain activation, phosphatidylserine exposure, cell blebbing and cell shrinkage. Eryptosis occurs physiologically in red blood cells, as a consequence of the natural senescence process of these cells, but it can also be stimulated in pathological situations such as metabolic syndromes, uremic syndromes, polycythemia vera, anemias such as sickle cell anemia and thalassemia, and infectious processes including Plasmodium infection. Infection-induced eryptosis is believed to contribute to damage caused by Plasmodium, but it’s still a topic of debate in the literature. In this review, we provided an overview of eryptosis mechanisms and its possible pathogenic role in malaria.

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“…Eryptosis contributes significantly to the pathophysiology of many diseases, since its activation is associated with the reduced lifespan of red blood cells and impaired blood clotting, which alters microcirculation [8]. Accelerated erythrocyte removal from the bloodstream via eryptosis favors the development of anemia in thalassemia [4], end stage renal disease [9], malaria [10], sickle cell anemia [2], etc. This suggests that the approaches used to manipulate eryptosis can be applied as therapeutic strategies in diseases associated with anemia [11].…”

Section: Introductionmentioning

confidence: 99%

Semi-refined carrageenan induces eryptosis in a Ca<sup>2+</sup>-dependent manner

Tkachenko¹,

Prokopіuk²,

Onishchenko³

2022

J CLIN MED KAZ

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Background: Semi-refined carrageenan (food additive E407a) is a widely used thickener, which has been reported to exert toxic and proinflammatory effects. In particular, there is accumulating evidence that it induces eryptosis, i.e. a programmed cell death of eryptocytes, via ROSmediated pathways. However, the role of Ca 2+ -dependent mechanisms in E407a-induced eryptosis is not elucidated.Material and methods: Semi-refined carrageenan at concentrations of 0 mg/ml, 1 mg/ml, 5 mg/ml and 10 mg/ml was incubated with blood of intact female WAG rats (n=9) for 24 h in RPMI and fetal bovine serum. After 24 h, the samples were used to obtain erythrocyte suspensions. The obtained suspensions were stained with a Ca 2+ -sensitive FLUO4 AM probe (30 min, 2.5 µM). The fluorescence of FLUO4 in erythrocytes was detected by a BD FACSCanto II flow cytometer.Results: The intracellular Ca 2+ levels are proportional to the fluorescence of FLUO4. The mean fluorescence intensities (MFI) were compared. Low levels (1 mg/ml) of E407a had no impact on Ca 2+ concentrations in erythrocytes (p>0.05). On the contrary, high concentrations (5 mg/ml and 10 mg/ml) of this food additive promoted an increase in the intracellular Ca 2+ levels. The MFI values were 2.3-and 2.5-fold higher, respectively (p<0.0001). In addition, the exposure to E407a at concentrations of 5 mg/ml and 10 mg/ml (p<0.0001) increased the percentage of cells with high FLUO4 fluorescence.Conclusion: Food additive E407a induces eryptosis in a Ca 2+dependent manner.

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Eryptosis of non-parasitized erythrocytes is related to anemia in Plasmodium berghei low parasitema malaria of Wistar rats

Cited by 3 publications

References 33 publications

Uncovering the mechanism of the effects of Paeoniae Radix Alba on iron-deficiency anaemia through a network pharmacology-based strategy

Uncovering the mechanism of the effects of Paeoniae Radix Alba on iron-deficiency anaemia through a network pharmacology-based strategy

Eryptosis as a New Insight in Malaria Pathogenesis

Semi-refined carrageenan induces eryptosis in a Ca<sup>2+</sup>-dependent manner

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