Eruca sativa seed extract modulates oxidative stress and apoptosis and up-regulates the expression of Bcl-2 and Bax genes in acrylamide-induced testicular dysfunction in rats

Abd‐Elsalam, Reham M.; Badawy, Shymaa A. El; Ogaly, Hanan A.; Ibrahim, Faten M.; Farag, Ola M.; Ahmed, Kawkab A.

doi:10.1007/s11356-021-14532-y

Cited by 24 publications

(17 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oxidative damage incurred by acrylamide is mostly associated with an increase in ROS production, as reported by others (42)(43)(44) and in this study. As known, lipid peroxidation due to oxidative stress is a cause of a decrease in antioxidant activity (45). This was confirmed by a depletion in the activity of SOD, GSH, and catalase and an increase in lipid peroxidation due to acrylamide administration.…”

Section: Discussionsupporting

confidence: 56%

The Protective Impact of Salsola imbricata Leaf Extract From Taif Against Acrylamide-Induced Hepatic Inflammation and Oxidative Damage: The Role of Antioxidants, Cytokines, and Apoptosis-Associated Genes

et al. 2022

View full text Add to dashboard Cite

Salsola imbricata is a herbal plant native to Saudi Arabia, known for its antioxidative and anti-inflammatory properties. This study explored the protective effects of an ethanolic leaf extract of Salsola imbricata against the oxidative stress and hepatic injury caused by acrylamide. Rats received intragastric administrations of 20 mg/kg of body weight of acrylamide to induce hepatic injury, or 300 mg/kg of body weight of Salsola ethanolic extract orally for 7 days before acrylamide administration. The treatments were continued for 3 weeks. Blood and liver samples were collected from all the groups, and the following biochemical parameters were tested: serum ALT (alanine aminotransferase), AST (aspartate aminotransferase), GGT (gamma glutaryl transferase), urea, albumin, total proteins, catalase, SOD (superoxide dismutase), reduced glutathione (GSH), nitric oxide (NO), and MDA (malondialdehyde). Quantitative real-time PCR (qRT-PCR) was used to examine the expression of Nrf2 (Nuclear factor-erythroid factor 2-related factor 2), HO-1 (Hemoxygenase-1), COX-2 (Cyclooxgenase-2), TGF-β1 (transforming growth factor-beta1), Bax, and Bcl2 (B-cell lymphoma 2), which are associated with oxidative stress, fibrosis, apoptosis, and anti-apoptotic effects. The annexin and survivin immunoreactivity were examined at the immunohistochemical level. Pretreatment with the Salsola ethanolic extract reduced the negative impact of acrylamide on ALT, AST, GGT, urea, albumin, and total proteins. The Salsola ethanolic extract reversed acrylamide's effects on serum and tissue antioxidants. Nrf2/HO-1 expression was downregulated, while COX-2 and TGF-β1 were upregulated in the acrylamide-administered group and normalized by the pre-administration of Salsola ethanolic extract to the acrylamide experimental group. The immunoreactivity of annexin and survivin was restored in the experimental group administered Salsola ethanolic extract plus acrylamide. In conclusion, Salsola ethanolic extract inhibits and regulates the side effects induced in the liver by acrylamide. Salsola induced its impacts by regulating inflammation, oxidative stress, and apoptosis-/anti-apoptosis-associated genes at the biochemical, molecular, and cellular levels. Salsola is recommended as oxidative stress relievers against environmental toixicity at high altitude areas.

show abstract

Section: Discussionsupporting

confidence: 56%

The Protective Impact of Salsola imbricata Leaf Extract From Taif Against Acrylamide-Induced Hepatic Inflammation and Oxidative Damage: The Role of Antioxidants, Cytokines, and Apoptosis-Associated Genes

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Tissue sections were stained with Mayer hematoxylin and mounted. INOS, COX2 and Caspase-3 protein expressions were measured and analyzed in stained areas in 7 random fields per group via image analyzer software (Image J, version 1.46a, NIH, Bethesda, MD, USA) [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk

Alsharif

Fayed

Abdel-Rahman

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

Ulcerative colitis (UC) is the most common type of inflammatory bowel disease, characterized by oxidative stress and elevated pro-inflammatory cytokines. Miconazole is an azole antifungal that stimulates the expression of antioxidant enzymes via Nrf2 activation, which consequently inhibits ROS formation and NF-κB activation. Hence, the present study aimed to investigate the protective effect of miconazole, sulfasalazine (as a reference drug) and their combination on acetic acid (AA)-induced UC in a rat model which was induced by intra-rectal administration of 4% AA. Rats were pretreated with miconazole (20 and 40 mg/kg, orally) or sulfasalazine (100 mg/kg, orally), or their combination (20 mg/kg miconazole and 50 mg/Kg of sulfasalazine, orally). Pretreatment with miconazole significantly reduced wet colon weight and macroscopic scores, accompanied by a significant amelioration of the colonic architecture disorder. Moreover, the treatment also significantly decreased the malondialdehyde (MDA) level and prevented the depletion of superoxide dismutase (SOD) activity and GSH content in inflamed colons. Additionally, the treatment showed suppressive activities on pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP), and upregulated the anti-inflammatory cytokine interleukin-10 (IL-10). Moreover, the treatment upregulated the protein levels of Nrf-2 and heme oxygenase-1 (HO-1) in the colon tissue. Taken together, miconazole is effective in alleviating AA-induced colitis in rats, and the mechanism of its action is associated with the activation of Nrf2-regulated cytoprotective protein expression.

show abstract

“…It is a monomer used in laboratories for preparation of polyacrylamide gel for electrophoresis and chromatographic techniques (Kirley & Norman, 2020). Strikingly, the discovery of ACR formation in carbohydrate‐rich foods processed at high temperature (>120°C) has raised significant human health concerns (Abd‐Elsalam et al, 2021). For example, the International Agency for Research on Cancer classifies ACR as a probable human carcinogen (Haidari et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Acrylamide is one of those environmental chemicals that exert deleterious effects on biological apparatus leading to human infertility. It is a germ cell mutagen and genotoxin that triggers damage to DNA in germ cells (Abd‐Elsalam et al, 2021; Nixon et al, 2012; Pourentezari et al, 2014). The study of Farag et al (2021) shows that ACR induces declined sperm quality, testicular degeneration, epididymis weight loss and aberrant steroidogenic signalling.…”

Section: Introductionmentioning

confidence: 99%

Silymarin abrogates acrylamide‐induced oxidative stress‐mediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats

Alturki

Famurewa

2022

Andrologia

View full text Add to dashboard Cite

Acrylamide (ACR) is a toxic chemical formed in foods processed at high temperature; it is a food‐borne toxicant with increasing public health attention due to its carcinogenic, neurotoxic and reproductive toxicities. However, till date, it is unknown whether silymarin (SIL) could attenuate ACR testicular toxicity. Therefore, the present study investigated the effect of SIL on ACR testiculotoxicity in rats. Rats were randomly divided and administered respective agents in Control group, ACR group, SIL group and ACR + SIL group for consecutive 14 days. Rat exposure to ACR resulted in significant reduction in the level of serum testosterone, whereas FSH and LH levels prominently increased compared to control. Acrylamide induced marked decreases in sperm count and sperm motility followed by a considerable increase in sperm abnormality percentage in the ACR‐exposed rats in comparison to control. The testicular activities of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) were significantly diminished, whereas malondialdehyde (MDA) level considerably increased. Additionally, ACR induced marked DNA fragmentation and histopathological lesions compared to control. Interestingly, the co‐treatment of SIL with ACR attenuated the altered reproductive indices and restored antioxidant balance and DNA integrity. Overall, SIL prevents ACR‐induced testicular reproductive deficits via modulation of antioxidant mechanism in rats.

show abstract

Eruca sativa seed extract modulates oxidative stress and apoptosis and up-regulates the expression of Bcl-2 and Bax genes in acrylamide-induced testicular dysfunction in rats

Cited by 24 publications

References 79 publications

The Protective Impact of Salsola imbricata Leaf Extract From Taif Against Acrylamide-Induced Hepatic Inflammation and Oxidative Damage: The Role of Antioxidants, Cytokines, and Apoptosis-Associated Genes

The Protective Impact of Salsola imbricata Leaf Extract From Taif Against Acrylamide-Induced Hepatic Inflammation and Oxidative Damage: The Role of Antioxidants, Cytokines, and Apoptosis-Associated Genes

Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk

Silymarin abrogates acrylamide‐induced oxidative stress‐mediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats

Contact Info

Product

Resources

About