Erste Beobachtungen mit dem Rasterelektronenmikroskop in der Fr�hphase der Endotoxinwirkung an der terminalen Strombahn

Problems with the preparation of blood vessels for scanning electron microscopy a critical review

“…physiological NaCl (Frost et al 1968, Hazama et al 1978; physiological NaCl at 40°C (Edanaga 1974); 0.9% NaCI, 5 min (Sharma & Geer, 1977); physiological NaCl + 0.1% EDTA , Sunaga et al 1969a,Sunuga et al 1969b); 0.9% NaCl + heparin (Swinehart et al 1976); Ringer's solution + heparin (Buss et al 1976); Hank's balanced salt solution (HBSS), pH 7.3 (Fishman et al 1975); phosphate buffered saline (PBS), pH 7.4 (Katora and Hollis 1976); Tyrode-solution, 10°C ; phosphate buffered Tyrode-solution p H 7.4 (Svendsen and Jorgensen 1978); Locke-Ringer-solution (Shimamoto et al 1978); 6 % macrodex in 5 % glucose solution -0.9% NaCl -HBSS (DeBruijn and Van Mourik 1975); phosphate buffer (PB) (Urbaschek et al 1969);PB, pH 7.4 (Gavin et al 1973); PB cold ; 0.1 m PB, pH 7.4 (Hirsch and Lazzarini Robertson 1977) ; DW + heparin, brought to 295 mOsmol with sucrose and NaHC03 (Van Benthuysen et al 1977).…”

“…Many authors have preferred the immersion method, some even to the present day (Dujovny et al 1978, Edanaga 1974, Fonkalsrud et al 1978, Shirnamoto et al 1969, Sunaga et al 1969a, 1969b, Urbaschek et al 1969, Weber and Tosi 1971a, and 1971b, Weber et al 1974. Here again the composition of solutions, the time, temperature, and the pH vary considerably (Tab.…”

“…With the SEM it is now possible to represent larger and more complicated surface relief structures, with a large depth of focus, over a wide range of magnifications and with relatively short processing times (Buss 1973). Publications from the late 1960's (Frost et al 1968, Shimamotu et al 1969, Sunaga et al 1969a, 1969b, Urbaschek et al 1969) already showed that the application of SEM techniques to biological materials could reveal important and unexpected morphological details. Including papers published in the recent past (Asmussen 1978, Dujovny et al 1978, Fonkalsrud et al 1976, Hazama et al 1978, Mayberg et al 1978, Reidy and Bowyer 1978a, Reidy and Bowyer 1978b, Svendsen and Jorgensen 1978 such a large number of studies have been published in the last few years that it is hardly possible to make a complete overview.…”