Error-promoting DNA synthesis in ovarian cancer cells

Dai, Heqiao; Hickey, Robert J.; Liu, Jianying; Bigsby, Robert M.; Lannér, Carita; Malkas, Linda H.

doi:10.1016/j.ygyno.2013.06.022

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…There are studies that have shown that prexasertib, a cell cycle checkpoint kinases 1 and 2 inhibitor, lead to synergistic cytotoxic effects against BRCA wild type high-grade serous ovarian neoplasms cells by reducing Rad51 foci formation and inducing apoptosis. Also, the systematic analysis of DNA replication process in ovarian neoplasms could reveal information on some molecular mechanisms of genetic damage accumulation and might contribute to the pathogenesis of the ovarian neoplasms [30,31].…”

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Pathway for Ovarian Neoplasms Using the OVDM1 Cell Line Based on Bioinformatics Analysis

Yin

Zhang

et al. 2019

Med Sci Monit

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Background Ovarian neoplasms are the fifth most common cancer affecting the health of women, and they are the most lethal gynecologic malignancies; however, the etiology of ovarian neoplasms remains largely unknown. There is an urgent need to further broaden the understanding of the development mechanism of ovarian neoplasms through in vitro research using different cell lines. Material/Methods To screen the differentially expressed genes (DEGs) that may play critical roles in OVDM1 (an ovarian cancer cell line), the public microarray data (GSE70264) were downloaded and screened for DEGs. Then, Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed. To screen hub genes, the protein–protein interaction network was constructed. The expression level and survival analysis of hub genes in patients with ovarian neoplasms were also analyzed. Results There were 79 upregulated and 926 downregulated DEGs detected, and the biological processes of the GO analysis were enriched in extracellular matrix organization, extracellular structure organization, and chromosome segregation, whereas, the KEGG pathway analysis was enriched in cell cycle and cell adhesion molecules. The hub gene BIRC5 , which might play a key role in ovarian neoplasms, was further screened. Conclusions The present study could deepen the understanding of the molecular mechanism of ovarian neoplasms using the OVDM1 cell line, which could be useful in developing clinical treatments of ovarian neoplasms.

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Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Pathway for Ovarian Neoplasms Using the OVDM1 Cell Line Based on Bioinformatics Analysis

Yin

Zhang

et al. 2019

Med Sci Monit

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“…In summary, germline and somatic mutations in the POLE and POLD1 genes appear to predispose to, or promote, colorectal and endometrial cancers in part by increasing the rates of SBSs [ 61 ]. Less direct investigations from cell culture nuclear extracts suggest that defects in DNA replication fidelity might also be associated with ovarian cancers [ 62 ].…”

Section: Meta-analyses Of Cancer Genomesmentioning

confidence: 99%

Mechanisms of Base Substitution Mutagenesis in Cancer Genomes

Bacolla

Cooper

Vásquez

2014

Genes

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Cancer genome sequence data provide an invaluable resource for inferring the key mechanisms by which mutations arise in cancer cells, favoring their survival, proliferation and invasiveness. Here we examine recent advances in understanding the molecular mechanisms responsible for the predominant type of genetic alteration found in cancer cells, somatic single base substitutions (SBSs). Cytosine methylation, demethylation and deamination, charge transfer reactions in DNA, DNA replication timing, chromatin status and altered DNA proofreading activities are all now known to contribute to the mechanisms leading to base substitution mutagenesis. We review current hypotheses as to the major processes that give rise to SBSs and evaluate their relative relevance in the light of knowledge acquired from cancer genome sequencing projects and the study of base modifications, DNA repair and lesion bypass. Although gene expression data on APOBEC3B enzymes provide support for a role in cancer mutagenesis through U:G mismatch intermediates, the enzyme preference for single-stranded DNA may limit its activity genome-wide. For SBSs at both CG:CG and YC:GR sites, we outline evidence for a prominent role of damage by charge transfer reactions that follow interactions of the DNA with reactive oxygen species (ROS) and other endogenous or exogenous electron-abstracting molecules.

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Error-promoting DNA synthesis in ovarian cancer cells

Cited by 2 publications

References 38 publications

Identification of Key Genes and Pathway for Ovarian Neoplasms Using the OVDM1 Cell Line Based on Bioinformatics Analysis

Identification of Key Genes and Pathway for Ovarian Neoplasms Using the OVDM1 Cell Line Based on Bioinformatics Analysis

Mechanisms of Base Substitution Mutagenesis in Cancer Genomes

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