Error Evaluation for Automated Diameter Measurements of Cerebral Capillaries Captured with Two-Photon Laser Scanning Fluorescence Microscopy

Suzuki, Hiroki; Sugashi, Takuma; Takeda, Hiroshi; Takuwa, Hiroyuki; Kanno, Iwao; Masamoto, Kazuto

doi:10.1007/978-3-030-48238-1_38

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…To reduce errors in diameter measurements, the capillaries were automatically segmented to exclude 6 mm from the junction point. 58 This omission may underestimate capillary behaviour in the vicinity of branches and make it difficult to quantify the absolute volume of the brain capillaries. Finally, capillary diameter was measured in a 3D reconstructed image with normalized intensity heterogeneity.…”

Section: Limitations Of the Methodsmentioning

confidence: 99%

Capillary responses to functional and pathological activations rely on the capillary states at rest

Suzuki

Takeda

Takuwa

et al. 2023

J Cereb Blood Flow Metab

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Brain capillaries play a crucial role in maintaining cellular viability and thus preventing neurodegeneration. The aim of this study was to characterize the brain capillary morphology at rest and during neural activation based on a big data analysis from three-dimensional microangiography. Neurovascular responses were measured using a genetic calcium sensor expressed in neurons and microangiography with two-photon microscopy, while neural acivity was modulated by stimulation of contralateral whiskers or by a seizure evoked by kainic acid. For whisker stimulation, 84% of the capillary sites showed no detectable diameter change. The remaining 10% and 6% were dilated and constricted, respectively. Significant differences were observed for capillaries in the diameter at rest between the locations of dilation and constriction. Even the seizures resulted in 44% of the capillaries having no detectable change in diameter, while 56% of the capillaries dilated. The extent of dilation was dependent on the diameter at rest. In conclusion, big data analysis on brain capillary morphology has identified at least two types of capillary states: capillaries with diameters that are relatively large at rest and stable over time regardless of neural activity and capillaries whose diameters are relatively small at rest and vary according to neural activity.

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Section: Limitations Of the Methodsmentioning

confidence: 99%

Capillary responses to functional and pathological activations rely on the capillary states at rest

Suzuki

Takeda

Takuwa

et al. 2023

J Cereb Blood Flow Metab

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Quantitative Assessment of Angiogenesis in Skin Wound Healing by Multi-Optical Imaging Techniques

Sun

et al. 2022

Front. Phys.

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The primary goal of wound healing therapy is to quickly close the wound and form healthy and functional scar tissue. As capillary system regeneration is an important condition for skin wound healing, it is important to dynamically monitor angiogenesis with multiple methods based on the 3D reconstruction of dermal microcirculation. In this study, we established a quantitative system to comprehensively evaluate angiogenesis and perfusion during wound healing. Full-thickness skin wounds were generated on the back of rats. Laser Doppler anemometer (LDA), laser speckle contrast imaging (LSCI), and medical electronic dermoscopy (MED) are used to observe the vasculature from the horizontal plane and quantify blood perfusion in wounds. We combined immunofluorescence staining and two-photon excited fluorescence microscopy (TPEF) to reconstruct 3D vessels for longitudinal quantification. These results indicated that new blood vessels around the wound grew parallel to the wound surface. The shape of the blood vessels changed from curved to straight, and the density of the surrounding vascular network gradually decreased as manifested by a decrease in the nodes and branches and the total length of the blood vessels. There were dense blood vessels that grew vertically to the wound surface in the center of the wound. This study established a real-time, non-invasive system to monitor re-epithelialization and angiogenesis and quantitatively assess blood vessels during wound healing, which facilitates the evaluation of new treatments for wound healing.

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Error Evaluation for Automated Diameter Measurements of Cerebral Capillaries Captured with Two-Photon Laser Scanning Fluorescence Microscopy

Cited by 2 publications

References 6 publications

Capillary responses to functional and pathological activations rely on the capillary states at rest

Capillary responses to functional and pathological activations rely on the capillary states at rest

Quantitative Assessment of Angiogenesis in Skin Wound Healing by Multi-Optical Imaging Techniques

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