Erratum zu: Auswirkungen von Natriumchlorid auf das Genexpressionsprofil parodontaler Ligamentfibroblasten bei Dehnung

Schröder, Agnes; Gubernator, Joshua; Nazet, Ute; Spanier, Gerrit; Proff, Peter; Kirschneck, Christian

doi:10.1007/s00056-021-00362-7

Cited by 2 publications

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“…Overt infiltration and inflammatory activity of myeloid cells, in particular Mφ, is associated with PD progression and severity. P. gingivalis (Pg) and A. actinomycetemcomitans (Aa) are two key etiological bacteria involved in periodontal pathogenesis (Grenier & Tanabe, 2010; Guentsch et al, 2009; Schröder et al, 2023). Information on lncRNA involvement in PD pathogenesis and the role of periodontal bacteria in lncRNA expressional perturbation remains poorly understudied (Ahmad et al, 2023; Sayad et al, 2020; Uttamani et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

Non‐coding RNA LINC01010 regulates macrophage polarization and innate immune functions by modulating NFκB signaling pathway

Valverde,

Naqvi,

Naqvi

2024

Journal Cellular Physiology

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Innate immune response is regulated by tissue resident or infiltrating immune cells such as macrophages (Mφ) that play critical role in tissue development, homeostasis, and repair of damaged tissue. However, the epigenetic mechanisms that regulate Mφ plasticity and innate immune functions are not well understood. Long non‐coding RNA (lncRNA) are among the most abundant class of transcriptome but their function in myeloid cell biology is less explored. In this study, we deciphered the regulatory role of previously uncharacterized lncRNAs in Mφ polarization and innate immune responses. Two lncRNAs showed notable changes in their levels during M1 and M2 Mφ differentiation. Our findings indicate that LINC01010 expression increased and AC007032 expression decreased significantly. LINC01010 exhibit myeloid cell‐specificity, while AC007032.1 is ubiquitous and expressed in both myeloid and lymphoid (T cells, B cells and NK cells) cells. Expression of these lncRNAs is dysregulated in periodontal disease (PD), a microbial biofilm‐induced immune disease, and responsive to lipopolysaccharide (LPS) from different oral and non‐oral bacteria. Knockdown of LINC01010 but not AC007032.1 reduced the surface expression of Mφ differentiation markers CD206 and CD68, and M1Mφ polarization markers MHCII and CD32. Furthermore, LINC01010 RNAi attenuated bacterial phagocytosis, antigen processing and cytokine secretion suggesting its key function in innate immunity. Mechanistically, LINC01010 knockdown Mφ treated with Escherichia coli LPS exhibit significantly reduced expression of multiple nuclear factor kappa B pathway genes. Together, our data highlight functional role of a PD‐associated lncRNA LINC01010 in shaping macrophage differentiation, polarization, and innate immune activation.

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Section: Resultsmentioning

confidence: 99%

Non‐coding RNA LINC01010 regulates macrophage polarization and innate immune functions by modulating NFκB signaling pathway

Valverde,

Naqvi,

Naqvi

2024

Journal Cellular Physiology

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“…Extracellular Na + content in tissues can trigger the expression of the osmoprotective transcription factor NFAT5 [ 31 , 32 , 33 , 34 ]. Moreover, NFAT5 expression is increased after mechanical loading in fibroblasts [ 35 ]. Sodium MRI studies have shown a correlation between the extracellular sodium concentrations and OA-characteristic catabolic remodeling processes of the extracellular matrix [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanical Stress Induces Sodium Entry and Osmoprotective Responses in Murine Synovial Fibroblasts

Proff,

Nazet,

Schröder

et al. 2024

Cells

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Osteoarthritis (OA) is a multifactorial disease depending on molecular, genetic, and environmental factors like mechanical strain. Next to the cartilage and the subchondral bone, OA also affects the synovium, which is critically involved in the maintenance of joint homeostasis. As there is a correlation between the extracellular sodium content in the knee joint and OA, this study investigates the impact of sodium on OA-associated processes like inflammation and bone remodeling without and with mechanical loading in synovial fibroblasts. For that purpose, murine synovial fibroblasts from the knee joint were exposed to three different extracellular sodium chloride concentrations (−20 mM, ±0 mM and +50 mM NaCl) in the absence or presence of compressive or intermittent tensile strain. In addition to the intracellular Na+ content and gene expression of the osmoprotective transcription factor nuclear factor of activated T cells 5 (Nfat5), the gene and protein expression of inflammatory mediators (interleukin-6 (IL6), prostaglandin endoperoxide synthase-2 (Ptgs2)/prostaglandin E2 (PGE2)), and factors involved in bone metabolism (receptor activator of NF-κB ligand (RANKL), osteoprotegerin (OPG)) were analyzed by qPCR and ELISA. Mechanical strain already increased intracellular Na+ and Nfat5 gene expression at standard salt conditions to levels obtained by exposure to increased extracellular Na+ content. Both high salt and compressive strain resulted in elevated IL6 and PGE2 release. Intermittent tensile strain did not increase Il6 mRNA expression or IL6 protein secretion but triggered Ptgs2 expression and PGE2 production. Increased extracellular Na+ levels and compressive strain increased RANKL expression. In contrast, intermittent tension suppressed RANKL expression without this response being subject to modification by extracellular sodium availability. OPG expression was only induced by compressive strain. Changes in extracellular Na+ levels modified the inflammatory response and altered the expression of mediators involved in bone metabolism in cells exposed to mechanical strain. These findings indicate that Na+ balance and Nfat5 are important players in synovial fibroblast responses to mechanical stress. The integration of Na+ and Na+-dependent signaling will help to improve the understanding of the pathogenesis of osteoarthritis and could lead to the establishment of new therapeutic targets.

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Erratum zu: Auswirkungen von Natriumchlorid auf das Genexpressionsprofil parodontaler Ligamentfibroblasten bei Dehnung

Abstract: The article "Effects of sodium chloride on the gene expression profile of periodontal ligament fibroblasts during tensile strain", written by

Cited by 2 publications

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Non‐coding RNA LINC01010 regulates macrophage polarization and innate immune functions by modulating NFκB signaling pathway

Non‐coding RNA LINC01010 regulates macrophage polarization and innate immune functions by modulating NFκB signaling pathway

Mechanical Stress Induces Sodium Entry and Osmoprotective Responses in Murine Synovial Fibroblasts

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