Erratum to “Ppm1E is an in cellulo AMP-activated protein kinase phosphatase” [Cell. Signalling 23 (2011) 114–124]

Voß, Martin; Paterson, James; Kelsall, Ian R.; Martín-Granados, Cristina; Hastie, C. James; Peggie, Mark; Cohen, Patricia T.W.

doi:10.1016/j.cellsig.2010.12.001

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“…However, it is previously reported that PPM1E rather than PP2Cα (PPM1A) is the major phosphatase for AMPK in HEK293 cells. 49 Therefore, we evaluated the enzymatic activity of purified PP2Cα in vitro upon addition of oxidized glutathione (GSSG), which did not change PP2Cα dephosphorylation activity significantly (Fig. S8B).…”

Section: Resultsmentioning

confidence: 99%

A clickable glutathione approach for identification of protein glutathionylation in response to glucose metabolism

et al. 2016

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Glucose metabolism and mitochondrial function are closely interconnected with cellular redox-homeostasis. Although glucose starvation, which mimics ischemic condition or insufficient vascularization, is known to perturb redox-homeostasis, global and individual protein glutathionylation in response to glucose metabolism or mitochondrial activity remains largely unknown. In this report, we use our clickable glutathione approach, which form clickable glutathione (azido-glutathione) by using a mutant of glutathione synthetase (GS M4), for detection and identification of protein glutathionylation in response to glucose starvation. We found that protein glutathionylation is readily induced in HEK293 cells in response to low glucose concentrations when mitochondrial reactive oxygen species (ROS) are elevated in cells, and glucose is the major determinant for inducing reversible glutathionylation. Proteomic and biochemical analysis identified over 1,300 proteins, including SMYD2, PP2Cα, and catalase. We further showed that PP2Cα is glutathionylated at C314 in a C-terminal domain, and PP2Cα C314 glutathionylation disrupts the interaction with mGluR3, an important glutamate receptor associated with synaptic plasticity

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Section: Resultsmentioning

confidence: 99%