Erratum to: Insight into the roles of hypoxanthine and thymidine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability

Chen, Fei; Li, Fan; Wang, Jiaqi; Zhou, Yan; Ye, Zhaoyang; Zhao, Liang; Tan, Wen-Song

doi:10.1007/s00253-012-3952-0

Cited by 7 publications

(13 citation statements)

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“…The anti-CD20 mAb was purified by Protein A affinity chromatography (HiTrap Protein A HP Columns, GE) and subsequently, the purified mAb concentration was quantified by reversed-phase HPLC as previously described by Chen et al (7). Lactate dehydrogenase (LDH) activity in the culture supernatant was detected by a commercial enzyme test kit (Nanjing JianCheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions.…”

Section: Routine Analytical Methodsmentioning

confidence: 99%

“…A CHO cell line expressing a chimeric anti-human CD20 monoclonal antibody (anti-CD20 mAb) under a DHFR expression system was used in this study (7). This cell line had previously been successfully adapted to suspension culture and cultivated in in-house developed animal component-free medium (named as CHO232 medium, Chinese patent published: CN101603026).…”

Section: Cell Line and Maintenance Of Cell Culturementioning

confidence: 99%

“…In our previous study, a 22% enhancement of antibody volumetric production was achieved through the combinatorial addition of 10 mg/L hypoxanthine and 2 mg/L thymidine in batch cultures (7). In the current study, we aimed to perform a more comprehensive study concerning the effects of hypoxanthine and thymidine (H&T) on cell growth and antibody production in a wider concentration range.…”

mentioning

confidence: 94%

“…However, Wong et al (6) observed no significant effects on CHO cell growth with 5 mM uridine (or 10 mM cytidine). Recently, our study (7) revealed that a combination of 10 mg/L hypoxanthine and 2 mg/L thymidine stimulated growth of CHO cells. The varied growth responses of cells to nucleotide addition may be attributed to the differences in cell lines and cell culture process.…”

mentioning

confidence: 97%

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Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production

Chen

Zhao

et al. 2012

Journal of Bioscience and Bioengineering

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Section: Routine Analytical Methodsmentioning

confidence: 99%

Section: Cell Line and Maintenance Of Cell Culturementioning

confidence: 99%

mentioning

confidence: 94%

mentioning

confidence: 97%

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Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production

Chen

Zhao

et al. 2012

Journal of Bioscience and Bioengineering

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“…The cell cycle was analyzed using a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA) coupled with CellQuest software as described previously (Chen et al 2012). The proportions of cells in G1, S, and G2/M were determined by ModFit LT software.…”

Section: Cell Cycle Analysis By Flow Cytometrymentioning

confidence: 99%

Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture

Sun

Liu

et al. 2015

Appl Microbiol Biotechnol

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Yeast extract (YE), as a non-animal source additive for mammalian cell culture medium, has been widely used for manufacturing of therapeutic proteins. In the present study, one particular YE was found to have significantly improved the specific productivity (q p) of Fc-fusion protein in recombinant Chinese hamster ovary (rCHO) cell culture. In order to elucidate the intracellular effects of YE on protein productivity, steps of the target protein synthesis process were investigated to unveil their variations caused by YE addition. Stepwise analysis on Fc-fusion protein synthesis process showed that YE enhanced Fc-fusion protein gene transcription with cell cycle arrest at G1 phase; mammalian target of rapamycin (mTOR) signaling pathway was activated to enhance the translation of Fc-fusion protein, and the block in post-translational steps of Fc-fusion protein was alleviated by YE addition as well. Our results revealed the responses of multiple protein production steps to the addition of YE and provided a practical guidance for the separation and application of active compounds from hydrolysates.

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Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system's productivity

Monteiro

Bernal

Saelens

et al. 2013

Biotech & Bioengineering

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Baculovirus infection boosts the host biosynthetic activity towards the production of viral components and the recombinant protein of interest, hyper-productive phenotypes being the result of a successful adaptation of the cellular network to that scenario. Spodoptera frugiperda derived Sf9 and Trichoplusia ni derived High Five cell lines have a major track record for the production of recombinant proteins, with High Five cells presenting higher productivities. A metabolic profiling of the two insect cell lines was pursued to underpin specific cellular traits behind productive phenotypes. Multivariate analysis identified cell-line dependent metabolic signatures linked to productivity. Pathway analysis highlighted cellular pathways of paramount importance in supporting infection and protein production. Moreover, better producer phenotypes proved to be correlated with the capacity of cells to shift their metabolism in favor of energy-generating pathways to fuel biosynthesis, a scenario observed in the High Five cell line. Metabolomic profiling allowed us to identify metabolic pathways involved in infection and recombinant protein production, which can be selected as targets for further improvement of the system.

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Erratum to: Insight into the roles of hypoxanthine and thymidine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability

Abstract: The original publication of this paper unfortunately contained an error. The word "thydimine" in the Article title must be replaced with "thymidine".The online version of the original article can be found at http://dx.doi.

Cited by 7 publications

References 0 publications

Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production

Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production

Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture

Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system's productivity

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