Erratum to “In vivo protein transduction: biologically active intact pep-1-superoxide dismutase fusion protein efficiently protects against ischemic insult” [Free Radic. Biol. Med. 37 (2004) 1656–1669]

Eum, Won Sik; Kim, Dae Won; Hwang, In Koo; Yoo, Ki‐Yeon; Kang, Tae‐Cheon; Jang, Sang Ho; Choi, Hee Soon; Choi, Soo Hyun; Kim, Young Hoon; Kim, So Young; Kwon, Hyeok Yil; Kang, Jung Hoon; Kwon, Oh Nam; Cho, Sung‐Woo; Le, Kil Soo; Park, Jinseu; Won, Moo Ho; Choi, Soo Young

doi:10.1016/j.freeradbiomed.2004.10.039

Cited by 43 publications

(70 citation statements)

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“…A major key for successful protein transfer into cells is the development of protein delivery systems with high efficiency and low cytotoxicity (19). Pep-1 as a cell penetrating peptide has been effectively used to transfer different cargoes (peptide nucleic acids or PNAs, peptides, and proteins) into the cells (20)(21)(22)(23)(24)(25)(26)Gros et al,personal communication). Several advantages of the noncovalent complexes of Pep-1/cargo contain: (a) rapid delivery of proteins into cells with high efficiency, (b) stability in physiological buffers, and (c) lack of toxicity and sensitivity to serum.…”

Section: Introductionmentioning

confidence: 99%

“…It involves transient membrane disorganization along with folding of Pep-1 into a helical structure within the phospholipids membrane (27,28). Many types of proteins, antibodies, and peptides were successfully delivered into different cell lines using Pep-1 strategy and were represented to retain their full biological activity (20)(21)(22)(23)(24)(25)(26)Gros et al,personal communication). In this study, the potency of Pep-1 cell penetrating peptide was assessed for HPV16 E7 protein delivery in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Protein vaccination with HPV16 E7/Pep‐1 nanoparticles elicits a protective T‐helper cell‐mediated immune response

et al. 2016

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Two human papillomavirus (HPV) viral oncoproteins, E6 and E7 represent ideal targets for development of a therapeutic HPV vaccine. It is important to reduce the rate of HPVassociated malignancies through improvement of vaccine modalities. In this study, we used a short amphipathic peptide carrier, Pep-1, for delivery of the full-length HPV16 E7 protein into mammalian cells and evaluated immune responses and protective effects of different formulations in C57BL/6 tumor mice model. Our results showed that the complexes of E7/ Pep-1 protein form stable nanoparticles through noncovalent binding with an average size of 120 to 250 nm. The efficient delivery of E7 protein by Pep-1 at molar ratio of 1:20 was detected in HEK-293T cell line for 1 h and 3 h posttransfection. Immunization with E7/Pep-1 nanoparticles at a ratio of 1:20 induced a higher Th1 cellular immune response with the predominant IgG2a and IFN-c levels than those induced by E7 protein in a murine tumor model. These data suggest that Pep-1 peptide would indicate promising applications for improvement of HPV therapeutic vaccines. V C 2016 IUBMB Life, 68(6): [459][460][461][462][463][464][465][466][467] 2016

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein vaccination with HPV16 E7/Pep‐1 nanoparticles elicits a protective T‐helper cell‐mediated immune response

et al. 2016

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“…Although Tat fusion proteins have been used to deliver therapeutic proteins in vitro and in vivo, the exact mechanism remains unclear. In previous studies, we have shown in vitro and in vivo that various transduced fusion proteins efficiently protected against cell death Choi et al, 2006a;2006b;Eum et al, 2004;Kim et al, 2009;Kwon et al, 2000).…”

Section: Introductionmentioning

confidence: 89%

Transduced Tat-DJ-1 Protein Protects against Oxidative Stress-Induced SH-SY5Y Cell Death and Parkinson Disease in a Mouse Model

Jeong

Kim

Woo

et al. 2012

Molecules and Cells

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Parkinson's disease (PD) is a well known neurodegenerative disorder characterized by selective loss of dopaminergic neurons in the substantia nigra pars compact (SN). Although the exact mechanism remains unclear, oxidative stress plays a critical role in the pathogenesis of PD. DJ-1 is a multifunctional protein, a potent antioxidant and chaperone, the loss of function of which is linked to the autosomal recessive early onset of PD. Therefore, we investigated the protective effects of DJ-1 protein against SH-SY5Y cells and in a PD mouse model using a cell permeable Tat-DJ-1 protein. Tat-DJ-1 protein rapidly transduced into the cells and showed a protective effect on 6-hydroxydopamine (6-OHDA)-induced neuronal cell death by reducing the reactive oxygen species (ROS). In addition, we found that Tat-DJ-1 protein protects against dopaminergic neuronal cell death in 1-methyl-4-phenyl-1,2,3,6,-tetrahydropyridine (MPTP)-induced PD mouse models. These results suggest that Tat-DJ-1 protein provides a potential therapeutic strategy for against ROS related human diseases including PD.

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“…Protein transduction technology based on small domains called protein transduction domains (PTDs) and cell penetrating peptides (CPPs) has been used to deliver exogenous protein into cultured cells and animal models (2)(3)(4)(5)(6)(7). Many research groups have demonstrated the efficacy of protein transduction technology for protein therapy strategies as well as drug delivery, although the exact mechanism of transduction remains unclear (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Although various proteins have been developed for protein therapy, protein transduction technology has several limitations such as transduction efficiency.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

Lee¹,

Jeong²,

Kim³

et al. 2010

BMB Reports

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Erratum to “In vivo protein transduction: biologically active intact pep-1-superoxide dismutase fusion protein efficiently protects against ischemic insult” [Free Radic. Biol. Med. 37 (2004) 1656–1669]

Cited by 43 publications

References 0 publications

Protein vaccination with HPV16 E7/Pep‐1 nanoparticles elicits a protective T‐helper cell‐mediated immune response

Protein vaccination with HPV16 E7/Pep‐1 nanoparticles elicits a protective T‐helper cell‐mediated immune response

Transduced Tat-DJ-1 Protein Protects against Oxidative Stress-Induced SH-SY5Y Cell Death and Parkinson Disease in a Mouse Model

Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

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