Erratum to: High Field Sodium MRI Assessment of Stem Cell Chondrogenesis in a Tissue-Engineered Matrix

Majumdar, Shreyan; Pothirajan, Padmabharathi; Dorcemus, Deborah; Nukavarapu, Syam P.; Kotecha, Mrignayani

doi:10.1007/s10439-016-1548-z

Cited by 1 publication

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“…Dr. Nukavarapu and his group have developed and effectively used this method to fabricate optimally porous and biomechanically compatible matrices (i.e., OTC matrices) for large-area bone regeneration 6,34,37 and gradient matrix systems for osteochondral tissue engineering. [38][39][40] Briefly, PLGA microspheres (diameter, 425-600 mm) and a porogen, NaCl crystals of diameter 200-300 mm, were mixed at a ratio of 4:1 by weight. The microsphere-salt mixture was packed into a steel mold and thermally sintered at 100°C for 1 h. NaCl particulates were leached out by soaking in water for 2 h. We fabricated and used disc-shaped matrices (10 mm diameter and 2 mm height) for in vitro studies and subcutaneous severe combined immunodeficiency (SCID) mice studies; we fabricated and used cylinder-shaped matrices (5 mm diameter and 15 mm height) for our critical-sized bone defect rabbit study model.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Oxygen Tension-Controlled Matrices with Osteogenic and Vasculogenic Cells for Vascularized Bone Regeneration In Vivo

Amini

Chidambaram

et al. 2016

Tissue Engineering Part A

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Despite recent progress, segmental bone defect repair is still a significant challenge in orthopedic surgery. While bone tissue engineering approaches using biodegradable matrices along with bone/blood vessel forming cells offered improved possibilities, current regenerative strategies lack the ability to achieve vascularized bone regeneration in critical-sized/segmental bone defects. In this study, we introduced and evaluated a two-pronged approach for vascularized bone regeneration in vivo. The goal was to demonstrate vascularized bone formation using oxygen tension-controlled (OTC) matrices seeded with bone and blood vessel forming cells. OTC matrices were coimplanted with rabbit mesenchymal stem cells (MSCs) and peripheral blood-derived endothelial progenitor cells (PB-EPCs) to demonstrate the osteogenic and vasculogenic differentiation of these cells, postseeding on a matrix, especially deep inside the matrix pore structure. Matrices coimplanted with varied rabbit MSC and PB-EPC ratios (1:4, 1:1, and 4:1) were assessed in a nude mouse subcutaneous implantation model to determine a coimplantation ratio with superior osteogenic as well as vasculogenic properties. The implants were analyzed, at week 8, for endothelial (CD31 and Von Willebrand factor [vWF]) and osteogenic marker (RunX2 and Col I) staining qualitatively and collagen deposition and number of vessel formation quantitatively. Results from these experiments established MSC-to-PB-EPC ratio 1:1 as the best coimplantation ratio. OTC matrix with 1:1 coimplantation ratio was assessed for segmental bone defect repair in a rabbit critical-sized bone defect model. The group under investigation was OTC matrix, and the matrix was seeded with MSCs, EPCs, or MSCs:EPCs in a 1:1 ratio. Explants at week 12 were evaluated for bone defect repair via micro-CT and histology. Results from rabbit in vivo experiments show enhanced mineralization and vascularization for the 1:1 coimplantation group. Overall, the study establishes a two-pronged approach involving OTC matrix and effective progenitors for large-area and vascularized bone regeneration.

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