Erratum to “Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation” Theriogenology 79 (2013) 842–846

Santiani, Alexei; Evangelista, Shirley; Valdivia, Martha; Risopatrón, Jennie; Sánchez, Raúl

doi:10.1016/j.theriogenology.2013.04.024

Cited by 8 publications

(17 citation statements)

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“…Reduced alpaca sperm motility after cryopreservation found in this study is similar to previous reports freeze/thawed epididymal (Morton et al., , ) and ejaculated spermatozoa (Santiani et al., , ). Furthermore, addition of antioxidants analogues of superoxide dismutase in alpaca semen extender prevents loss of motility and DNA fragmentation (Santiani et al., ).…”

Section: Discussionsupporting

confidence: 93%

“…Then, samples were loaded into 0.25 ml French straws. Straws were kept at 5°C for 30 min for stabilization of cryoprotectant, then exposed to liquid nitrogen vapour for 15 min and finally plunged into liquid nitrogen (Santiani et al., ). Straws were thawed in water bath at 37°C for 1 min, 24 hours after cryopreservation.…”

Section: Methodsmentioning

confidence: 99%

“…In alpacas, there are some detailed studies on ejaculated (Santiani, Evangelista, Valdivia, Risopatrón, & Sánchez, ; Santiani et al., ) or epididymal sperm (Banda et al., ; Morton, Bathgate, Evans, & Maxwell, ; Morton, Evans, & Maxwell, ) cryopreservation. However, a successful cryopreservation protocol for alpaca spermatozoa has not been developed.…”

Section: Introductionmentioning

confidence: 99%

“…Acceptable pregnancy rates (60%) using artificial insemination (AI) with diluted semen are reported in alpacas (Alarcón, García, & Bravo, ). Nevertheless, AI with frozen semen in alpacas is not commonly used because of low sperm motility percentages (0%–20%) in alpaca spermatozoa after cryopreservation (Santiani et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…Probably, there is also oxidative stress during cryopreservation of alpaca semen, because the use of antioxidants SOD analogues during this process improves post‐thaw motility and reduces DNA fragmentation (Santiani et al., ). Additionally, superoxide dismutase (SOD) can reduce oxidative damage during cryopreservation of ram spermatozoa (Santiani et al., ) and prevent sperm physiological effects mediated by ROS in human spermatozoa (De Lamirande, Tsai, Harakat, & Gagnon, ).…”

Section: Introductionmentioning

confidence: 99%

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Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Evangelista‐Vargas

Santiani

2017

Reprod Domestic Animals

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The objective of this study was to detect changes in intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) production and lipid peroxidation during cryopreservation of alpaca spermatozoa. Twelve alpaca semen samples were conventionally cryopreserved. Intracellular superoxide anion and hydrogen peroxide were evaluated by fluorescence microscopy using dihydroethidium (DHE)/YO-PRO-1 and dichlorofluorescein diacetate (H2DCFDA)/propidium iodide (PI), respectively. Evaluations were performed during cooling curve at (1) 25°C, (2) 15°C, (3) 5°C/0 min, (4) 5°C/15 min, (5) 5°C/30 min and (6) after freezing/thawing. Evaluation of lipid peroxidation by measuring malondialdehyde (MDA) was performed at 25°C, 5°C/30 min and after thawing. Maximum percentages of total spermatozoa producing superoxide anion and hydrogen peroxide were found at 5°C/30 min (62.8 ± 6.3% and 30.5 ± 5.6%, respectively), and these results were higher (p < .05) than initial (25°C: 10.8 ± 3.8% and 6.8 ± 0.7%, respectively) and after thawing (29.8 ± 9.5% and 7.5 ± 1.8%, respectively) values. However, considering only viable spermatozoa, production of superoxide anion and hydrogen peroxide during overall stabilization at 5°C (>76% and >91%, respectively) and after thawing (74.9 ± 5.0% and 78.9 ± 2.2%, respectively) was higher (p < .05) than initial values at 25°C (38.7 ± 3.1% and 53.6 ± 2.0%, respectively). Lipid peroxidation at 25°C, 5°C/30 min, and post-thawing were 346.5 ± 99.8, 401.1 ± 64.8 and 527.7 ± 142.8 ng/ml MDA, respectively. These results showed that high percentage of viable alpaca spermatozoa produces intracellular reactive species oxygen (ROS) during the cryopreservation process of alpaca semen.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Evangelista‐Vargas

Santiani

2017

Reprod Domestic Animals

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Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing

Najafi

Mehdipour

Mohammadi

et al. 2022

Sci Rep

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The aim of the present study was to investigate the effects of tempol and straw size on rooster sperm post-thaw quality and fertility. Rooster semen was cryopreserved in Lake extender containing 0 (control), 5, 10, 15 and 20 μM tempol (in two different straw size, 0.25 and 0.5). The percentage of total and progressive sperm motility, VAP and VSL increased in the 10 µM tempol group. Moreover, 10 µM tempol led to lower ROS compared to other groups. The lowest percentage of apoptotic-like changes was detected when the extender was treated with 10 µM of tempol. The minimum ROS was observed in the group treated with 0.5 straw size. Straw size did not have any significantly effect on GPx and SOD activities and TAC of frozen-thawed sperm. The highest significant percentage of fertility and hatching rate were observed in 10 µM of tempol. The results of the present study showed that supplementation of the Lake cryopreservation medium with 10 µM tempol improved cryo-survival. Also, the results of the present study suggested that Lake cryopreservation medium with 0.5-ml straw may perhaps be an appropriate method to improve the quality and fertility post-thawed rooster sperm.

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Evaluation of the cryoprotective effect of seminal plasma on llama ( Lama glama) spermatozoa

et al. 2019

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In South American camelids, sperm survival is low after thawing and poor results are obtained when artificial insemination is performed with cryopreserved semen. The aim of this study was to evaluate the effect of different percentages (10% and 50%) of seminal plasma added prior to the process of cryopreservation and also to evaluate the absence of seminal plasma on llama sperm survival after freezing and thawing. A total of 15 ejaculates from five adult llama males (n = 5; r = 3) were evaluated. A significant decrease in sperm motility, viability, membrane function and intact acrosomes was observed in thawed samples (0%, 10% and 50%) when compared to raw semen. Neither morphology nor chromatin condensation was altered in all thawed samples (p > 0.05), but a significant increase (p < 0.05) in the percentage of spermatozoa with fragmented DNA was observed after thawing all samples compared to raw semen. Higher percentages of total and progressive sperm motility were observed when 0% and 10% of seminal plasma were used compared to 50%. However, no statistical differences were established for sperm viability, membrane function, morphology, acrosome status and DNA quality between thawed treatments. To conclude, neither of the percentages of seminal plasma used showed superiority nor cryoprotective effect on llama sperm survival.

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Erratum to “Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation” Theriogenology 79 (2013) 842–846

Cited by 8 publications

References 0 publications

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing

Evaluation of the cryoprotective effect of seminal plasma on llama ( Lama glama) spermatozoa

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