Erratum to: Cultivation-independent analysis of archaeal and bacterial communities of the formation water in an Indian coal bed to enhance biotransformation of coal into methane

Singh, D; Kumar, Ashok; Sarbhai, M. P.; Tripathi, Anil Kumar

doi:10.1007/s00253-012-3879-5

Cited by 2 publications

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“…in CBM produced water and also associated with coal samples from PRB, Wyoming. Furthermore, many other studies also reported findings of Methanobacteriales in a variety of deep subsurface coal basins such as the Jhari Gippsland basin, Australia (Midgley et al, 2010), Alberta coal beds of Canada (Penner et al, 2010), and coal fields located at Parbatpur in India (Singh et al, 2012). After ∼2.5 years of routine transfers of CBM produced water into methanogenic media, the enrichments were highly dominated by two species of Methanobacterium sp., which are M. subterraneum and M. formicicum .…”

Section: Discussionmentioning

confidence: 93%

“…While hydrogenotrophic Methanobacterium ssp. were found in enrichment cultures from the Jharia coal field, India (Singh et al, 2012), and Methanosarcinales (Green et al, 2008), Methanobacteriales, and Methanococcales (Klein et al, 2008) were discovered in the PRB, Wyoming. Some studies questioned whether members of the methanogenic community could be enhanced for methane production by manipulating temperature, pH, coal particle surface, electron acceptors or donors, or rapid release of organic substrates (Green et al, 2008; Harris et al, 2008; Jones et al, 2010; Penner et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Trace Elements Affect Methanogenic Activity and Diversity in Enrichments from Subsurface Coal Bed Produced Water

Ünal¹,

Perry²,

Sheth³

et al. 2012

Front. Microbio.

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Microbial methane from coal beds accounts for a significant and growing percentage of natural gas worldwide. Our knowledge of physical and geochemical factors regulating methanogenesis is still in its infancy. We hypothesized that in these closed systems, trace elements (as micronutrients) are a limiting factor for methanogenic growth and activity. Trace elements are essential components of enzymes or cofactors of metabolic pathways associated with methanogenesis. This study examined the effects of eight trace elements (iron, nickel, cobalt, molybdenum, zinc, manganese, boron, and copper) on methane production, on mcrA transcript levels, and on methanogenic community structure in enrichment cultures obtained from coal bed methane (CBM) well produced water samples from the Powder River Basin, Wyoming. Methane production was shown to be limited both by a lack of additional trace elements as well as by the addition of an overly concentrated trace element mixture. Addition of trace elements at concentrations optimized for standard media enhanced methane production by 37%. After 7 days of incubation, the levels of mcrA transcripts in enrichment cultures with trace element amendment were much higher than in cultures without amendment. Transcript levels of mcrA correlated positively with elevated rates of methane production in supplemented enrichments (R2 = 0.95). Metabolically active methanogens, identified by clone sequences of mcrA mRNA retrieved from enrichment cultures, were closely related to Methanobacterium subterraneum and Methanobacterium formicicum. Enrichment cultures were dominated by M. subterraneum and had slightly higher predicted methanogenic richness, but less diversity than enrichment cultures without amendments. These results suggest that varying concentrations of trace elements in produced water from different subsurface coal wells may cause changing levels of CBM production and alter the composition of the active methanogenic community.

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Section: Discussionmentioning

confidence: 93%