Introduction: Knowledge of HIV and other STIs and their complications and attitude toward sexual health are important in planning preventive and treatment strategies. Although STIs pose serious risks to health security especially to women, there is a dearth of literature quantifying the knowledge of HIV and STIs among women in Nigeria. The aim of this study was to assess the knowledge of HIV and other STIs and its association with the choice of place of delivery among urban women in south west Nigeria.Methodology: National Demographic Survey (DHS) use largely standardized questionnaires and methodologies and cover a range of topics. The surveys are nationally representative and include men and women aged 15–49 years old and children under the age of 5 years residing in non-institutional settings. The 2018 NDHS survey data for women only was used for this study. The outcome variable was knowledge of HIV and other sexually transmitted infection while the explanatory variable was location of most recent childbirth. Following descriptive analysis was conducted using proportions and frequency. Chi-square (χ2) test and binary logistic regression analysis were conducted to establish association between variables. Data were analysed with Statistical Package for the Social Sciences (SPSS) version 25.Result: Four thousand six hundred and four (4604) women were surveyed, 33.1% were between 30 – 39 years of age with a mean age of 34.4±9.4, 50.9% had secondary education, 58.6% were married, 43.8% had 1 – 3 children, 47.8% and 48.2% had their first and second delivery at a public health facility. Some (21.9%) of the respondents had poor knowledge of HIV and other sexually transmitted diseases while 78.1% had good knowledge. Age, level of education, marital status, and partners level of education were significantly associated with knowledge of HIV and other STIs. Women between 30-39 years of age and had secondary school education were 1.7 times and 2.6 time more likely to have knowledge of HIV and other STIs respectively (OR=1.737; p<0.001; CI=1.402 - 2.153; OR=2.604; p<0.001; CI=1.934 - 3.507). Statistically significant association between knowledge of HIV and place of birth. Women who delivered their first child in public healthcare facilities were 1.7 times more likely to have good knowledge of HIV and STIs (OR=1.674; p<0.001; CI=1.233 - 2.275). Conclusion: Women education is an important factor for knowledge of HIV and other STIs which is also associated with choice of place of delivery. To bridge the gap in the knowledge of HIV among urban women, it is essential to implement more educational programmes specifically targeted at less educated women in urban regions of Nigeria to increase the knowledge on HIV and other STIs.