Erratum: Overview of the yeast genome

Abstract

The collaboration of more than 600 scientists from over 100 laboratories to sequence the Saccharomyces cerevisiae genome was the largest decentralised experiment in modern molecular biology and resulted in a unique data resource representing the first complete set of genes from a eukaryotic organism. 12 million bases were sequenced in a truly international effort involving European, US, Canadian and Japanese laboratories. While the yeast genome represents only a small fraction of the information in today's public sequence databases, the complete, ordered and non-redundant sequence provides an invaluable resource for the detailed analysis of cellular gene function and genome architecture. In terms of throughput, completeness and information content, yeast has always been the lead eukaryotic organism in genomics; it is still the largest genome to be completely sequenced.

Inverted repeat of a large segment unveiled on the right arm of Saccharomyces cerevisiae chromosome II

“…Our conclusion is obviously contradictory to that of the yeast genome project (Mewes et al, 1997). According to their map, ATP3 and YBR030W are single-copy genes.…”

“…The nuclear genes encoding the F 1 sector subunits are all single-copy genes, according to the yeast genome project (Mewes et al, 1997). However, it has been found that ATP1 (Takeda et al, 1995(Takeda et al, , 1999 and ATP2 (Takeda, personal (Ohnishi et al, 2003) is a duplicate gene.…”

Gene Expression Analysis

“…The entire genome of S. cer6isiae has been sequenced and all genes, of both known and putative function, have been identified (19). At present, cDNA arrays containing the complete set of 6,144 open reading frames (ORFs) for S. cer6isiae have been constructed, with only about 25% having a known function.…”

Verification of a new gene on Saccharomyces cerevisiae chromosome III

“…Saccharomyces cerevisiae was the first eukaryote whose genome was completely sequenced (Mewes et al, 1997;Goffeau et al, 1996). Chromosome III, the first eukaryotic chromosome to be completed, was a landmark in the field of genomic sequencing (Oliver et al, 1992).…”

“…The initial gene annotation of S. cerevisiae was based on identification of open reading frames (ORFs) of more than 100 amino acids (Mewes et al, 1997) and on prediction of introns using the well established splicing rules for this fungus (Brian and Micheal, 1992). The 100 amino acid size cut-off was picked in order to avoid fortuitous ORFs (Termier and Kalogeropoulos, 1996).…”