Stream biofi lms are among the fi rst to react to environmental degradation, since their structural and functional characteristics are tightly linked to the physicochemical variables in the water and sediment. The objectives of this research were to study the differences in chlorophyll-a, bacterial density and metabolism endpoints of epipelic biofi lms in nutrient-rich streams under different physical-chemical conditions in the stream water in relation to changes in urbanization, and to measure the short-term responses (up to 72 h) in the biofi lm when translocated to more urbanized sites. For these purposes, chlorophyll-a, bacterial density, biofi lm respiration (electron transfer activity) and O 2 consumption were measured in epipelic biofi lms in nutrient-rich streams exposed to different levels of urbanization after a 30 day colonization. Afterward, biofi lms were translocated downstream to more polluted sites, and sampled to identify any fast occurring changes to be considered as potential indicators of environmental degradation. Results show that in the nutrient-rich streams studied, the structural characteristics of the biofi lm were linked to urbanization, and even after a short time following the translocation, chlorophyll-a and bacterial density varied, refl ecting the environmental degradation. On the other hand, metabolic variables were highly variable and produced inconsistent results when representing an increase in urbanization.