Erratum for “A high‐throughput screen for antibiotic drug discovery” (Volume 111, issue 2, pp. 232–243)

Scanlon, Thomas C.; Dostal, Sarah M.; Griswold, Karl E.

doi:10.1002/bit.26840

Cited by 1 publication

(1 citation statement)

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“…Microfluidic techniques have shown great potential for point-of-care testing (POCT) applications owing to their advantages of high integration, low reagent usage, and rapid detection [1]. In the past decades, microfluidic devices have been widely used for on-site detection of molecules, cells, pathogens, and microorganisms in different scenarios, such as pregnancy detection, food hygiene detection, and COVID-19 virus detection [2][3][4][5][6][7][8]. In conventional microfluidic systems, various microfluidic handlings of liquid samples, e.g., pumping, stopping, mixing, and washing, are usually required for different purposes, in which external or internal liquid control units, such as mechanical (electro, magnetic, etc.)…”

Section: Introductionmentioning

confidence: 99%

A 3D Capillary-Driven Multi-Micropore Membrane-Based Trigger Valve for Multi-Step Biochemical Reaction

Zhang

Luan

et al. 2022

Biosensors

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Point-of-care testing (POCT) techniques based on microfluidic devices enabled rapid and accurate tests on-site, playing an increasingly important role in public health. As the critical component of capillary-driven microfluidic devices for POCT use, the capillary microfluidic valve could schedule multi-step biochemical operations, potentially being used for broader complex POCT tasks. However, owing to the reciprocal relationship between the capillary force and aperture in single-pore microchannels, it was challenging to achieve a high gating threshold and high operable liquid volume simultaneously with existing 2D capillary trigger valves. This paper proposed a 3D capillary-driven multi-microporous membrane-based trigger valve to address the issue. Taking advantage of the high gating threshold determined by micropores and the self-driven capillary channel, a 3D trigger valve composed of a microporous membrane for valving and a wedge-shaped capillary channel for flow pumping was implemented. Utilizing the capillary pinning effect of the multi-micropore membrane, the liquid above the membrane could be triggered by putting the drainage agent into the wedge-shaped capillary channel to wet the underside of the membrane, and it could also be cut off by taking away the agent. After theoretical analysis and performance characterizations, the 3D trigger valve performed a high gating threshold (above 1000 Pa) and high trigger efficiency with an operable liquid volume above 150 μL and a trigger-to-drain time below 6 s. Furthermore, the retention and trigger states of the valve could be switched for repeatable triggering for three cycles within 5 min. Finally, the microbead-based immunoreaction and live cell staining applications verified the valve’s ability to perform multi-step operations. The above results showed that the proposed 3D trigger valve could be expected to play a part in wide-ranging POCT application scenarios.

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