Erratum: correction: Autonomic healing of polymer composites

Abstract

PPARa were determined by chemical-mediated fluorescence energy transfer assays using the AlphaScreen Technology from Packard BioScience 30. The experiments were conducted with 5 nM PPARa LBD of biotinylated peptide containing individual motifs (Fig. 3a), following the manufacturer's instructions for the hexahistidine detection kit in a buffer containing 50 mM MOPS, pH 7.4, 50 mM NaF, 0.05 mM CHAPS, 0.1 mg ml-1 bovine serum albumin, and 10 mM dithiothreitol (DTT). The binding signals were detected with the increasing concentrations of GW6471, and the results from four repeated experiments were normalized as a percentage of the binding in the absence of GW6471. The effects of GW6471 on the affinity of the SMRT or N-CoR peptides with purified PPARa LBD were determined by fluorescence polarization in a buffer containing 10 mM HEPES, pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% polysorbate-20, 5 mM DTT and 2.5% DMSO. Varied concentration of PPARa LBD in the presence or absence of 40 mM GW6471 were incubated at room temperature with 10 nM of a fluorescein-labelled peptide of N-CoR2 or SMRT2 (Fig. 3a). The fluorescence polarization values for each concentration of receptor were determined using a BMG PolarStar Galaxy fluorescence reader with 485 nm excitation and 520 nm emission filters. The apparent dissociation constant (K d) values were determined by the binding curves derived from a nonlinear least-squares-fit of the data for a simple 1:1 interaction. Mutational analysis of the SMRT co-repressor motif interaction with the PPARa and TRb LBDs was also performed by fluorescence polarization. To determine the importance of each amino acid in the SMRT motif for binding to nuclear receptors, SMRT peptides with alanine substitution at each position were added to inhibit the binding of 1 mM TRb LBD or 2 mM PPARa to the fluorescent N-CoR2 peptide. For the PPARa experiments we added 10 mM GW6471. The inhibition curves were constructed and IC 50 values were determined by nonlinear least-squares-fit of the data to a simple 1:1 interaction.

Recent Approaches for Designing Nanomaterials-Based Coatings for Corrosion Protection

“…However, in the case of extrinsic self-healing, the healing agent is encapsulated within a reservoir [42,43]. The encapsulated healing agent contains liquid monomer that is able to polymerize at room temperature with the help of a catalyst present in the composite matrix [44,45]. However, there are some cases that the self-healing agent can polymerize without any catalytic support [46].…”

Self-Healing Epoxy Composites Based on the Use of Nanoporous Silica Capsules

“…Here we elaborate on a self-healing approach in which special healing capsules are embedded in the polymer matrix (1)(2)(3)(4). When a crack propagates it ruptures the capsules, healing glue leaks out into the crack, sealing, and "curing" the crack.…”

A Delivery System for Self‐Healing Inorganic Films

“…While the idea of self-healing has been around for many years, recent studies on the autonomic healing of structural polymers has brought about a new wave of research. [23][24][25][26][27][28][29] White et al for example have achieved the phenomenon of self-healing through the encapsulation of a healing agent within microcapsules and dispersed these encapsulations with a catalyst in a polymer matrix. [29] Further studies using this approach include the development of a 3D microvascular network embedded within a polymeric substrate that has shown the ability to regain some of the original structural rigidity after repeated damaging events.…”

“…[23][24][25][26][27][28][29] White et al for example have achieved the phenomenon of self-healing through the encapsulation of a healing agent within microcapsules and dispersed these encapsulations with a catalyst in a polymer matrix. [29] Further studies using this approach include the development of a 3D microvascular network embedded within a polymeric substrate that has shown the ability to regain some of the original structural rigidity after repeated damaging events. [30] While these studies present a novel method for the autonomic healing of polymer matrices, the application of this strategy to self-healing metal oxides is not possible since the fundamental property of metal oxide thin films in permeation barriers that makes them appealing is their ability to exclude water and oxygen, a feat that polymeric healing cannot achieve.…”