Erratum: A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk

Steck, Kathrin; Veit, Daniel; Grandy, Ronald; Badia, Sergi Bermúdez i; Mathews, Zenon; Verschure, Paul F. M. J.; Hansson, Bill S.; Knaden, Markus

doi:10.1038/srep01495

Cited by 3 publications

(6 citation statements)

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“…More recently, the range of focusing behaviors within these complex secondary flows in spirals and nearly constant curvature channels has been characterized (48, 51, 56, 57). In general, the behavior of particles’ equilibrium positions have been shown to move toward the center of curvature and then away at higher flow rates.…”

Section: Essential Forcesmentioning

confidence: 99%

“…The working theory is that the redistribution of the velocity profile, a consequence of Dean flow, causes a vertical shift of the equilibrium position, which moves away from the center of curvature as the particle experiences Dean flow in that direction (33, 34, 59). This three-dimensional (3D) motion has been studied in cylindrical curved channels (55) as well as in spiral geometry (48) and is still an area of active research.…”

Section: Essential Forcesmentioning

confidence: 99%

“…Essentially, the change in height from low (toward the center of curvature) to high (away from the center of curvature) gives rise to a gradient in Dean flow velocities across the width of the channel. This causes a sharp transition, dependent on particle size, in the equilibrium position of a particle as the flow rate is increased (48). In curved channels, this shape allows for a different Dean flow field, essentially a gradient in drag force across the channel, which improves size-based separation (48, 61, 63).…”

Section: Cross-sectional Shapementioning

confidence: 99%

“…The second type of separation is more common and has been used for the isolation of circulating tumor cells from blood based upon some cancer cell populations being larger than all other naturally occurring blood cells (see Figure 6 b ) (63, 66, 96, 100–105). This type of separation has been applied more generically to other types of cellular suspensions in which a size difference is apparent, including human mesenchymal stem cells, whose size varies significantly with stage in the cell cycle (48, 58, 106, 107). Fungal cell samples (e.g., Saccharomyces cerevisiae ) can also be separated based upon their shape and size, both of which depend on growth-cycle stage (75).…”

Section: Applicationsmentioning

confidence: 99%

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Inertial Focusing in Microfluidics

Martel

Toner

2014

Annu. Rev. Biomed. Eng.

451

516

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When Segré and Silberberg in 1961 witnessed particles in a laminar pipe flow congregating at an annulus in the pipe, scientists were perplexed and spent decades learning why such behavior occurred, finally understanding that it was caused by previously unknown forces on particles in an inertial flow. The advent of microfluidics opened a new realm of possibilities for inertial focusing in the processing of biological fluids and cellular suspensions and created a field that is now rapidly expanding. Over the past five years, inertial focusing has enabled high-throughput, simple, and precise manipulation of bodily fluids for a myriad of applications in point-of-care and clinical diagnostics. This review describes the theoretical developments that have made the field of inertial focusing what it is today and presents the key applications that will make inertial focusing a mainstream technology in the future.

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Section: Essential Forcesmentioning

confidence: 99%

Section: Essential Forcesmentioning

confidence: 99%

Section: Cross-sectional Shapementioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

See 2 more Smart Citations

Inertial Focusing in Microfluidics

Martel

Toner

2014

Annu. Rev. Biomed. Eng.

451

516

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“…Figure 3a–d are the composite bright field images illustrating the complete footprint of MC focusing before bifurcation. First, we investigated a scaled-up trapezoidal spiral with a low slant (Tan(α) = 0.075) that is similar to trapezoidal spirals reported at the micron scale 25 , 33 . Though the MC volume fraction is as low as ~0.2%, the relatively large band of MC streaks equilibrated at the inner half of the cross-section (Fig.…”

Section: Resultsmentioning

confidence: 99%

Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions

Moloudi

Yang

et al. 2018

Sci Rep

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Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5).

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