Errata to: xenobiotic detoxification in the nematode <i>Caenorhabditis elegans</i>

Lindblom, Tim; Dodd, Allyn K.

doi:10.1002/jez.528

Cited by 17 publications

(23 citation statements)

References 4 publications

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“…GSTs secreted by PWN into the host participate in the detoxification of host-derived defence compounds and enable successful parasitism [ 44 ]. Based on the known functions of these proteins and our current results, these genes may perform key detoxifying roles during the first and second stages of xenobiotic metabolism leading to resistance to β-pinene, consistent with previous results on C. elegans [ 42 ].…”

Section: Discussionsupporting

confidence: 92%

“…Heme-containing CYP-450 proteins are a ubiquitous family of monooxygenase isozymes responsible for the oxidative metabolism of a wide variety of endogenous and exogenous substrates [ 41 ]. SDRs are also important for detoxification metabolism in nematodes, especially in the early stages of the detoxification process [ 29 , 42 ].GSTs are multifunctional proteins that are essential for xenobiotic metabolism as well as detoxification of endogenous compounds, and they provide protection against peroxidative damage . UGTs and GSTs are the main enzymes mediating phase 2 metabolism in C. elegans , in which the actual detoxification reactions of xenobiotic metabolism take place [ 29 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…SDRs are also important for detoxification metabolism in nematodes, especially in the early stages of the detoxification process [ 29 , 42 ].GSTs are multifunctional proteins that are essential for xenobiotic metabolism as well as detoxification of endogenous compounds, and they provide protection against peroxidative damage . UGTs and GSTs are the main enzymes mediating phase 2 metabolism in C. elegans , in which the actual detoxification reactions of xenobiotic metabolism take place [ 29 , 42 ]. The study found that the expression level of GST gene in PWN treated with high-concentration β-pinene was higher than that in PWN treated with low concentration, and both were lower than the control.…”

Section: Discussionmentioning

confidence: 99%

“…ABC family members and other channels and transporters mediate the final stages of detoxification metabolism [ 42 ]. ABC transporters are transmembrane proteins that transport various substrates across extra- and intracellular membranes, including metabolic products, lipids and sterols, and drugs and their metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Adaptation of pine wood nematode Bursaphelenchus xylophilus to β-pinene stress

Feng

Wang

et al. 2020

BMC Genomics

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Background The pine wood nematode (PWN; Bursaphelenchus xylophilus ) is the most damaging biological pest in pine forest ecosystems in China. However, the pathogenic mechanism remains unclear. Tracheid cavitation induced by excess metabolism of volatile terpenes is a typical characteristic of pine trees infected by B. xylophilus . β-pinene, one of the main volatile terpenes, influences PWN colonization and reproduction, stimulating pathogenicity during the early stages of infection. To elucidate the response mechanism of PWN to β-pinene, pathogenesis, mortality, and reproduction rate were investigated under different concentrations of β-pinene using a transcriptomics approach. Results A low concentration of β-pinene (BL, C < 25.74 mg/ml) inhibited PWN reproduction, whereas a high concentration (BH, C > 128.7 mg/ml) promoted reproduction. Comparison of PWN expression profiles under low (BL, 21.66 mg/ml) and high (BH, 214.5 mg/ml) β-pinene concentrations at 48 h identified 659 and 418 differentially expressed genes (DEGs), respectively, compared with controls. Some key DEGs are potential regulators of β-pinene via detoxification metabolism (cytochrome P450, UDP-glucuronosyltransferases and short-chain dehydrogenases), ion channel/transporter activity (unc and ATP-binding cassette families), and nuclear receptor -related genes. Gene Ontology enrichment analysis of DEGs revealed metabolic processes as the most significant biological processes, and catalytic activity as the most significant molecular function for both BL and BH samples. Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) analysis showed that xenobiotics biodegradation and metabolism, carbohydrate metabolism, lipid metabolism, amino acid metabolism, metabolism of cofactors and vitamins, and transport and catabolism were the dominant terms in metabolism categories. Conclusion In addition to detoxification via reduction/oxidation (redox) activity, PWN responds to β-pinene through amino acid metabolism, carbohydrate metabolism, and other pathways including growth regulation and epidermal protein changes to overcome β-pinene stress. This study lays a foundation for further exploring the pathogenic mechanism of PWN.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Adaptation of pine wood nematode Bursaphelenchus xylophilus to β-pinene stress

Feng

Wang

et al. 2020

BMC Genomics

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“…In the nematode antioxidant pathway, daf-16 and skn-1 act together to regulate the expression of genes such as superoxide dismutase (SOD) and catalase (CTL), genes that encode glutathione peroxidases (GPX) and peroxiredoxin (PRDX) (Gillet et al, 2017). Xenobiotic/endobiotic detoxification pathway, also mediated by daf-16 and/or skn-1, is activated against internal or external toxic compounds (Gillet et al, 2017) and has a role in the detoxification and excretion of these compounds (Lindblom and Dodd, 2009). Basso et al, 2020 used in planta RNAi technology to silence the daf-16 and skn-1 transcription factors and achieved significant resistance to root knot nematode Meloidogyne incognita.…”

Section: Xenobiotic Metabolism To Counteract Plant Resistancementioning

confidence: 99%