ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells

Ouyang, Ming; Liu, Hongrui; Zhu, Xingang; Yang, Qing

doi:10.1016/j.biochi.2011.01.017

Cited by 21 publications

(16 citation statements)

References 25 publications

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“…Radixin is a cytoskeletal protein that might affect the cytoskeletal element assembly at the cytoplasmic face of the membrane and the nuclear skeleton, which would facilitate cell invasion. These results agree with another report where ERM stable knockdown by shRNA reduced in vitro human SGC-7901 cell migration and invasion (Ou-Yang et al, 2011). Angiogenesis is another essential step for tumor growth and metastasis.…”

Section: Discussionsupporting

confidence: 93%

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin¹,

Wang²,

Du³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

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Section: Discussionsupporting

confidence: 93%

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin¹,

Wang²,

Du³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…25 Silencing of RDX has also been found to suppress the migration and invasion of GC cells. 40 Together with our findings, these results suggested that RDX could act as a pro-metastatic factor in various cancers. Our study indicates that miR-409 can regulate the expression of RDX in GC.…”

Section: Discussionsupporting

confidence: 87%

MicroRNA-409 suppresses tumour cell invasion and metastasis by directly targeting radixin in gastric cancers

et al. 2011

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Emerging evidence has shown that aberrantly expressed microRNAs (miRNAs) are highly associated with tumour development and progression. However, little is known about the potential role of miRNAs in gastric cancer (GC) metastasis. In this study, miR-409-3p was found to be downregulated frequently in human GCs, and its expression was significantly associated with tumor-node-metastasis (TNM) stage and lymph node metastasis. Enforced expression of miR-409 in GC cells significantly reduced their migration and invasion in vitro and their capacity to develop distal pulmonary metastases and peritoneal dissemination in vivo. Moreover, we found that miR-409 exerted its function predominantly through the mature miR-409-3p, but not miR-409-5p. Microarray and bioinformatics analysis identified the pro-metastatic gene radixin (RDX) as a potential miR-409-3p target. Further studies confirmed that miR-409-3p suppressed the expression of RDX by directly binding to its 3 0 -untranslated region. Silencing of RDX by small interfering RNAs phenocopied the effects of miR-409 overexpression, whereas restoration of RDX in miR-409-overexpressed GC cells reversed the suppressive effects of miR-409. Taken together, these results demonstrate that miR-409 suppresses GC cell invasion and metastasis by directly targeting RDX and that patients with downregulated miR-409-3p are prone to lymph node metastasis.

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“…Moesin is a cytoskeletal protein that appears to affect cytoskeletal assembly between the cytoplasmic side of the cell membrane and the nuclear skeleton, which would facilitate cell invasion (17,22). In the present study, we found that β-catenin expression was affected by moesin expression in glioblastoma cells, suggesting involvement of β-catenin in moesin's effects on glioblastoma-cell proliferation and invasion.…”

Section: Effect Of Moesin Up-regulation In Cell Glioblastoma Invasionsupporting

confidence: 53%

“…Moesin is involved in cell morphology, motility and adhesion, as well as other processes of tumorigenesis (10)(11)(12). Moesin has been studied in estrogen receptor (ER)-negative breast cancers (13), basal breast carcinomas (14), pancreatic cancer (15), melanoma (16), and gastric carcinoma (17). Moesin expression correlated with pathologic grade and poor clinical outcome, including survival in astrocytoma (18,19).…”

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confidence: 99%

Moesin Expression Is Associated with Glioblastoma Cell Proliferation and Invasion

Wang

Zhao

et al. 2017

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Abstract. Aim: To investigate the effect of moesin expression on cell proliferaton and invasion of human glioblastoma cell lines in vitro. Materials and Methods: Glioblastoma LN229 and U87 cells were transfected with the H4645-plenti-EGFP-moesin expression vector for moesin up-regulation. Moesin and β-catenin expression levels in the transfectedGlioblastoma is a highly invasive recalcitrant cancer that is poorly understood (1-9). Moesin is a member of the ezrinradixin-moesin (ERM) protein family and is a link between the actin cytoskeleton and the plasma membrane. Moesin is involved in cell morphology, motility and adhesion, as well as other processes of tumorigenesis (10-12). Moesin has been studied in estrogen receptor (ER)-negative breast cancers (13), basal breast carcinomas (14), pancreatic cancer (15), melanoma (16), and gastric carcinoma (17). Moesin expression correlated with pathologic grade and poor clinical outcome, including survival in astrocytoma (18,19). However, the role of moesin in human glioblastoma progression is incompletely understood and, thus, the topic of the present report.In this study, we investigated moesin's effect on the growth and invasion ability of the glioblastoma cells by upregulation of the gene (MSN). Materials and MethodsCell culture. The glioblastoma cell line LN229 and U87 were purchased from the Shanghai cell bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in RPMI 1640 medium (GIBCO Life Technologies, Grand Island, NY, USA) containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific) at 37˚C with 5% CO 2 .Moesin-expression vector transfection. The H4645-plenti-enhanced green fluorescent protein (EGFP)-moesin expression vector was used for up-regulation of the moesin gene (MSN) in the LN229 and U87 glioblastoma cells (LN229-H4645 and U87-H4645, respectively). The H149 plenti-EGFP empty vector was used as negative control (LN229-H149 and U87-H149, respectively). The moesin expression vector was constructed by Obio Technology Corp., Ltd. (Shanghai, China). Cultured glioblastoma LN229 and U87 cells were transfected with the moesin expression vector according to the manufacturer's instructions. After 24h transfection, the medium was changed. Cells were then observed under fluorescence microscopy at 48 hours after transfection. Cells were selected and treated with 500-800 μg/ml G148 (Micropoly Biotech Co., Ltd, Nanjing, China) for 1 week. Images were obtained from fluorescence microscopy. Transfection efficiency of the expression vector in the two cell lines was 2211 This article is freely accessible online.

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ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells

Cited by 21 publications

References 25 publications

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

MicroRNA-409 suppresses tumour cell invasion and metastasis by directly targeting radixin in gastric cancers

Moesin Expression Is Associated with Glioblastoma Cell Proliferation and Invasion

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