ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled

Miyatake, Koichi; Kusakabe, Morioh; Takahashi, Chika; Nishida, Eisuke

doi:10.1038/ncomms7666

Cited by 37 publications

(49 citation statements)

References 51 publications

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“…A dense actin meshwork is present at the apical cortex in MCCs and myosin is also enriched in this area (Reverdin et al 1975;Reed et al 1984;Klotz et al 1986;Lemullois et al 1988;Sandoz et al 1988;Park et al 2006Park et al , 2008Pan et al 2007;Werner et al 2011). The formation of the apical actin network is temporally linked to BB docking and involves conserved actin regulators, such as the RhoA and Rac1GTPases, the membrane-cytoskeletal linker ezrin, the cross-linking protein filamin A, the nucleator dishevelled-associated activator of morphogenesis 1 (DAAM1), or the F-actin capping protein CapZ-interacting protein (CapZIP) (Gomperts et al 2004;Pan et al 2007;Park et al 2008;Chevalier et al 2015;Epting et al 2015;Miyatake et al 2015;Yasunaga et al 2015). The expression of several of these actin regulators is triggered by the regulatory network that controls MCC differentiation (Fig.…”

Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

“…In addition, inturned recruits the PCP effector DAAM1, and Dvl facilitates the activation of CapZIP by the kinase extracellular signal-regulated kinase 7 (ERK7) (Miyatake et al 2015;Yasunaga et al 2015). Another potential PCP effector affecting the actin cytoskeleton is the recently characterized flattop (FLTP) protein, which is required for BB docking in mouse MCCs (Gegg et al 2014).…”

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

“…The expression of several of these actin regulators is triggered by the regulatory network that controls MCC differentiation (Fig. 1) (Gomperts et al 2004;Pan et al 2007;Chevalier et al 2015;Miyatake et al 2015). Apical actin assembly and BB docking in MCCs are orchestrated by the planar cell polarity (PCP) pathway.…”

Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

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Multiciliated Cells in Animals

Meunier¹,

Azimzadeh²

2016

Cold Spring Harb Perspect Biol

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Many animal cells assemble single cilia involved in motile and/or sensory functions. In contrast, multiciliated cells (MCCs) assemble up to 300 motile cilia that beat in a coordinate fashion to generate a directional fluid flow. In the human airways, the brain, and the oviduct, MCCs allow mucus clearance, cerebrospinal fluid circulation, and egg transportation, respectively. Impairment of MCC function leads to chronic respiratory infections and increased risks of hydrocephalus and female infertility. MCC differentiation during development or repair involves the activation of a regulatory cascade triggered by the inhibition of Notch activity in MCC progenitors. The downstream events include the simultaneous assembly of a large number of basal bodies (BBs)-from which cilia are nucleated-in the cytoplasm of the differentiating MCCs, their migration and docking at the plasma membrane associated to an important remodeling of the actin cytoskeleton, and the assembly and polarization of motile cilia. The direction of ciliary beating is coordinated both within cells and at the tissue level by a combination of planar polarity cues affecting BB position and hydrodynamic forces that are both generated and sensed by the cilia. Herein, we review the mechanisms controlling the specification and differentiation of MCCs and BB assembly and organization at the apical surface, as well as ciliary assembly and coordination in MCCs.

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Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

Section: Multiciliated Cells In Animalsmentioning

confidence: 99%

Section: Role Of the Actin Cytoskeleton In Bb Migration And Docking Tmentioning

confidence: 99%

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Multiciliated Cells in Animals

Meunier¹,

Azimzadeh²

2016

Cold Spring Harb Perspect Biol

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“…ERK7 is the Xenopus ortholog of C. elegans MAPK‐15, which was recently linked to ciliary assembly defects during Xenopus development (Miyatake et al, ). Basal body migration defects were observed in erk7 deficient cells, likely involving interactions with actin at the cell surface.…”

Section: Discussionmentioning

confidence: 99%

“…Miyatake, Kusakabe, Takahashi, & Nishida () recently showed that the Xenopus MAPK‐15 ortholog ERK7 is involved in basal body positioning through interactions with the actin regulator CapZIP in multiciliated cells. However, MAPK‐15 orthologs are found in ciliated organisms with reduced basal body structure, that lack CapZIP orthologs, and that do not contain multiciliated cells, including animals like nematode worms.…”

Section: Introductionmentioning

confidence: 99%

MAPK‐15 is a ciliary protein required for PKD‐2 localization and male mating behavior in Caenorhabditis elegans

et al. 2017

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Cilia are conserved cellular structures that facilitate sensory-based processes, including those required for neuronal and kidney functions. Here, we show that the human mitogen activated kinase-15 (MAPK-15) ortholog in Caenorhabditis elegans encodes a ciliary protein. A strain harboring a mutation in the catalytic site of the kinase domain results in ciliary-specific defects in tail neurons of both hermaphrodite and male worms, manifesting in dye uptake, dendrite extension, and male mating behavior defects. Transgenic-fusion constructs for two mapk-15 isoforms (A and C) with full-length kinase domains were generated. Expression of either the A- or C-specific isoform rescues the dye-filling and male-mating defective phenotypes, confirming the ciliary function of mapk-15. Expression of mapk-15 occurs in many ciliated-sensory neurons of the head and tail in hermaphrodite and male worms. Localization of MAPK-15 isoforms A and C occurs in the cell body, dendritic processes, and cilia. A C. elegans ortholog of polycystin-2, a protein that when defective in mammals results in autosomal dominant polycystic kidney disease, is mislocalized in the male ray neurons of mapk-15 mutant worms. Expression of the mapk-15 gene by the pkd-2 promoter partially rescues the male-mating defects observed in mapk-15 mutant animals. Expression of mapk-15 is DAF-19/RFX dependent in some CSNs and DAF-19/RFX independent in others. Collectively, these data suggest that MAPK-15 functions upstream of PKD-2 localization to modulate ciliary sensory functions.

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Cellular and physiological roles of the conserved atypical MAP kinase ERK7

2022

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Extracellular signal‐regulated kinase 7 (ERK7), also known as ERK8 and MAPK15, is an atypical member of the MAP kinase family. Compared with other MAP kinases, the biological roles of ERK7 remain poorly understood. Recent work, however, has revealed several novel functions for ERK7. These include a highly conserved essential role in ciliogenesis, the ability to control cell growth, metabolism and autophagy, as well as the maintenance of genomic integrity. ERK7 functions through phosphorylation‐dependent and ‐independent mechanisms and it is activated by cellular stressors, including DNA‐damaging agents, and nutrient deprivation. Here, we summarize recent developments in understanding ERK7 function, emphasizing its conserved roles in cellular and physiological regulation.

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ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled

Cited by 37 publications

References 51 publications

Multiciliated Cells in Animals

Multiciliated Cells in Animals

MAPK‐15 is a ciliary protein required for PKD‐2 localization and male mating behavior in Caenorhabditis elegans

Cellular and physiological roles of the conserved atypical MAP kinase ERK7

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