ERK1/2 Mediated The Vasodilatation of Apelin-13 on Vascular Rings of Spontanously Hypertensive Rat &lt;I&gt;in vitro&lt;/I&gt;*

LIU, Chang-Hui; Chen, Linxi; Li, Xin; Chen, Feng; Pan, Weinan; Feng, Fen; Qin, Xu; LI, Lan-Fang; Su, Tao

doi:10.3724/sp.j.1206.2009.01578

Cited by 1 publication

(1 citation statement)

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“…Our laboratory has been studied the effect of apelin/APJ on the cardiovascular system for several years. We demonstrated that apelin‐13 reduced blood pressure (Li et al, ) and promoted vasodilatation of vascular rings on spontaneously hypertension rat in vitro which was mediated by extracellular signal–regulated kinases 1 and 2 (ERK1/2) (Liu et al, ). Besides, we also reported that apelin‐13 promoted cardiomyocytes (Xie et al, ).…”

Section: Discussionmentioning

confidence: 99%

ROS‐Autophagy pathway mediates monocytes‐human umbilical vein endothelial cells adhesion induced by apelin‐13

Liu

Zhou

et al. 2018

Journal Cellular Physiology

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Apelin is the endogenous ligand of APJ receptor. Both monocytes (MCs) and human umbilical vein endothelial cells (HUVECs) express apelin and APJ, which play important roles in the physiological processes of atherosclerosis. Our previous research indicated that apelin-13 promoted MCs-HUVECs adhesion. Here, we further explore the mechanism responsible for MCs-HUVECs adhesion induced by apelin-13. Apelin-13 promoted reactive oxygen species (ROS) generation and NOX4 expression in HUVECs. Apelin-13 inducedautophagy, increased proteins beclin1 and LC3-II/I expression and induced autophagy flux in HUVECs, which was blocked by NAC, catalase and DPI. Autophagy flux induced by apelin-13 was inhibited by NAC and catalase but not hydroxychloroquine (HCQ). NAC, catalase, and DPI prevented apelin-13 induced ICAM-1 expression in HUVECs. Rapamycin enhanced MCs-HUVECs adhesion that was reversed by NAC, catalase, and DPI. Down-regulation of beclin1 and LC3 by siRNA blocked MCs-HUVECs adhesion. Apelin-13 induced atherosclerotic plaque and increased NOX4, LC3-II/I expression in ApoE-/-(HFD) mouse model. Our results demonstrated that apelin-13 induced MCs-HUVECs adhesion via a ROS-autophagy pathway.

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Section: Discussionmentioning

confidence: 99%