ERK-dependent and -independent pathways trigger human neural progenitor cell migration

Interleukin-7 (IL-7) and IL-7 splice variants affect differentiation of human neural progenitor cells

“…NHNP neurospheres represent heterogeneous, self-regulating cell populations of human neural precursor and neural stem cells defined by the expression of nestin and other early neural markers. 22 RNA expression analysis showed that NHNP neurospheres express IL-7c and the splice variants IL-7d4 and d3/4 ( Figure 3a); they express the full-length IL-7R and the IL-7R splice variants IL-7d6 and IL-7Rd5/6 ( Figure 3a). IL-7 protein was detected by staining with an anti-IL-7-specific mAb (Figure 3b), yet this does not allow the discrimination of IL-7 splice variants, as monoclonal and polyclonal reagents recognize several IL-7 isoforms.…”

“…and glia cells for glial fibrillary acidic protein (GFAP) (red) as described. 22 (b) The mean increase in the number of GFAP-positive cells relative to the number of neurons as a percentage of four independent experiments. Five neurospheres were used for each treatment group (that is, IL-7c or IL-7 isoforms) in every experiment.…”

“…22 After washing in phosphate-buffered saline, spheres were incubated overnight in a 10% sucrose solution (w/v) at 4 1C. The neurospheres were then transferred to a freezing medium (Tissue Tek, Sakura Finetek Europe B.V., Histolab Products AB, Gö teborg, Sweden).…”

Grouping of histone deacetylase inhibitors and other toxicants disturbing neural crest migration by transcriptional profiling

“…Test systems able to explore a larger set of compounds including groups of structurally and/or mechanistically related compounds useful for QSAR or read across approaches are rare. An additional limitation for mechanistic studies can be that a particular system evaluates an integrated endpoint affected by cell migration, cell division and cell differentiation at the same time (Moors et al, 2007). Since mechanistic conclusions are difficult or impossible, if several different biological processes contribute to the overall readout, we have shown earlier that the MINC assay faithfully assesses cell migration independent of differentiation and cell division .…”

State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology

“…they may then, e.g., be used for electrophysiological studies or to follow differentiation during radial migration on a laminin-coated surface (Moors et al, 2007(Moors et al, , 2009van Vliet et al, 2007). It is noteworthy that despite differentiation onto a surface, this manner of differentiation differs from simple 2D single cell plating because there is glial cell guidance with neuronal growth on top in a processorganized manner out of an organoid cell system (Ahlenius and Kokaia, 2010;Bal-Price et al, 2012;liu and Sun, 2011;Schreiber et al, 2010).…”