Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Abstract

<p class="Abstract">The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein.</p><p><strong>Video Clip</strong></p><a href="https://youtube.com/v/MJwBotwrgWM">Flow cytometry assay</a>: 35 sec<p> </p>

Eriodictyol induces apoptosis via regulating phosphorylation of JNK, ERK, and FAK/AKT in pancreatic cancer cells

“…PARP plays an important role in DNA repair and is one of the markers of mitochondria-mediated apoptosis [28]. Moreover, eriodictyol induces apoptosis through cleavage of PARP in human hepatocellular carcinoma Hep-G2 cells [23]. Our results are consistent with studies carried out previous.…”

“…f.) [19], sutil lemon (Citrus aurantiifolia) [20], and bergamot orange (Citrus bergamia Risso) [21]. Eriodictyol has anticancer effects in liver, lung, and colorectal cancer cells [22][23][24], but its effects in pancreatic cancer remain unknown. Hence, in this study, we investigated the mechanisms associated with the anticancer effect of eriodictyol in pancreatic cancer cells.…”

Investigation of Anti-Liver Cancer Activity of the Herbal Drug FDY003 Using Network Pharmacology

“…Cordycepin targets integrin, focal adhesion kinase (FAK), c-Jun N-terminal kinases (JNK), PI3K/Akt/mammalian target of rapamycin (mTOR), nuclear factor erythroid-2-related factor 2 (Nrf2)/ heme oxygenase 1 (HO-1)/nuclear factor kappa-light-chainenhancer of activated B cells (NF-κB), IL-6, IL-1β, TNF-α, extracellular-signal-regulated kinase (ERK), Fas, B-cell lymphoma-2 (Bcl-2), caspase, and C-X-C motif chemokine receptor (CXCR) 4 cascades, thereby leading to the repression of angiogenesis, metastasis, EMT, proliferation, viability, and invasion of LC cells [147][148][149][150][151][152][153]. Eriodyctiol induces cell cycle arrest and apoptosis of LC cells by targeting the poly ADP-ribose polymerase (PARP), Bcl-2, and BCL2-associated X (Bax) signaling [154]. Isorhamnetin and β-sitosterol suppress the survival, viability, and proliferation of LC cells [155,156].…”

Stability of lysozyme incorporated into electrospun fibrous mats for wound healing

“…10). Apoptotic cells were determined by the signs of change of nucleus from round and homogenous chromatin to polygonal, detached or sparse, shrinkage, condensed chromatin (54,55).…”