Eriocheir sinensis vesicle-associated membrane protein can enhance host cell phagocytosis to resist Spiroplasma eriocheiris infection

Wang, Yaqin; Miao, Yanyang; Shen, Qijun; Liu, Xueshi; Chen, Minyi; Du, Jie; Ning, Mingxiao; Bi, Jian; Gu, Wei; Wang, Li; Meng, Qingguo

doi:10.1016/j.fsi.2022.08.012

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…Following fusion with lysosomes to generate phagolysosomes, engulfed pathogens are eventually degraded by abundant hydrolytic enzymes 62,63 . In our previous study, host cell phagocytosis was confirmed as a defense mechanism against S. eriocheiris infection in E. sinensis 64 . Recent evidence showed that RNAi‐mediated knockdown of CD63 in Paphia undulate caused a dramatic reduction in hemocyte phagocytic activity against Vibrio parahaemolyticus and Vibrio alginolyticus 65 .…”

Section: Discussionmentioning

confidence: 89%

“…62,63 In our previous study, host cell phagocytosis was confirmed as a defense mechanism against S. eriocheiris infection in E. sinensis. 64 Recent evidence showed that RNAi-mediated knockdown of CD63 in Paphia undulate caused a dramatic reduction in hemocyte phagocytic activity against Vibrio parahaemolyticus and Vibrio alginolyticus. 65 And the phagocytic rate of hemocytes toward Vibrio splendidus and Yarrowia lipolytica was significantly reduced after incubation with an anti-CD63 antibody.…”

Section: Estetraspanin Antibody Neutralization Reduces Phagocytosis T...mentioning

confidence: 99%

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Hemolymph exosomes inhibit Spiroplasma eriocheiris infection by promoting Tetraspanin‐mediated hemocyte phagocytosis in crab

Ma,

Yao,

Meng

et al. 2024

The FASEB Journal

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Exosomes released from infected cells are thought to play an important role in the dissemination of pathogens, as well as in host‐derived immune molecules during infection. As an intracellular pathogen, Spiroplasma eriocheiris is harmful to multiple crustaceans. However, the immune mechanism of exosomes during Spiroplasma infection has not been investigated. Here, we found exosomes derived from S. eriocheiris‐infected crabs could facilitate phagocytosis and apoptosis of hemocytes, resulting in increased crab survival and suppression of Spiroplasma intracellular replication. Proteomic analysis revealed the altered abundance of EsTetraspanin may confer resistance to S. eriocheiris, possibly by mediating hemocyte phagocytosis in Eriocheir sinensis. Specifically, knockdown of EsTetraspanin in E. sinensis increased susceptibility to S. eriocheiris infection and displayed compromised phagocytic ability, whereas overexpression of EsTetraspanin in Drosophila S2 cells inhibited S. eriocheiris infection. Further, it was confirmed that intramuscular injection of recombinant LEL domain of EsTetraspanin reduced the mortality of S. eriocheiris‐infected crabs. Blockade with anti‐EsTetraspanin serum could exacerbate S. eriocheiris invasion of hemocytes and impair hemocyte phagocytic activity. Taken together, our findings prove for the first time that exosomes modulate phagocytosis to resist pathogenic infection in invertebrates, which is proposed to be mediated by exosomal Tetraspanin, supporting the development of preventative strategies against Spiroplasma infection.

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Section: Discussionmentioning

confidence: 89%

Section: Estetraspanin Antibody Neutralization Reduces Phagocytosis T...mentioning

confidence: 99%

Hemolymph exosomes inhibit Spiroplasma eriocheiris infection by promoting Tetraspanin‐mediated hemocyte phagocytosis in crab

Ma,

Yao,

Meng

et al. 2024

The FASEB Journal

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“…The Chinese mitten crab Eriocheir sinensis is an important freshwater species in the Chinese aquaculture industry due to its high commercial value ( Chen and Zhang, 2007 ). Thus far, many approaches have been applied to study the immunity, development and metabolism of these crabs, including investigations of single gene or global gene functionality by high-throughput sequencing analysis ( Robalino et al., 2009 ; Wang et al., 2022 ). The combination of whole genome sequencing and transcriptome analysis is a highly effective approach with which to perform systematic studies of gene function.…”

Section: Introductionmentioning

confidence: 99%

Full-length transcriptome sequencing and comparative transcriptome analysis of Eriocheir sinensis in response to infection by the microsporidian Hepatospora eriocheir

Hou

Wang

Zhu

et al. 2022

Front. Cell. Infect. Microbiol.

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As a new generation of high-throughput sequencing technology, PacBio Iso-Seq technology (Iso-Seq) provides a better alternative sequencing method for the acquisition of full-length unigenes. In this study, a total of 22.27 gigabyte (Gb) subread bases and 128,614 non-redundant unigenes (mean length: 2,324 bp) were obtained from six main tissues of Eriocheir sinensis including the heart, nerve, intestine, muscle, gills and hepatopancreas. In addition, 74,732 unigenes were mapped to at least one of the following databases: Non-Redundant Protein Sequence Database (NR), Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG), KEGG Orthology (KO) and Protein family (Pfam). In addition, 6696 transcription factors (TFs), 28,458 long non-coding RNAs (lncRNAs) and 94,230 mRNA-miRNA pairs were identified. Hepatospora eriocheir is the primary pathogen of E. sinensis and can cause hepatopancreatic necrosis disease (HPND); the intestine is the main target tissue. Here, we attempted to identify the key genes related to H. eriocheir infection in the intestines of E. sinensis. By combining Iso-Seq and Illumina RNA-seq analysis, we identified a total of 12,708 differentially expressed unigenes (DEUs; 6,696 upregulated and 6,012 downregulated) in the crab intestine following infection with H. eriocheir. Based on the biological analysis of these DEUs, several key processes were identified, including energy metabolism-related pathways, cell apoptosis and innate immune-related pathways. Twelve selected genes from these DEUs were subsequently verified by quantitative real-time PCR (qRT-PCR) analysis. Our findings enhance our understanding of the E. sinensis transcriptome and the specific association between E. sinensis and H. eriocheir infection.

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Integrated Information for Pathogenicity and Treatment of Spiroplasma

You,

Xiao,

Chen

et al. 2024

Curr Microbiol

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Eriocheir sinensis vesicle-associated membrane protein can enhance host cell phagocytosis to resist Spiroplasma eriocheiris infection

Cited by 6 publications

References 40 publications

Hemolymph exosomes inhibit Spiroplasma eriocheiris infection by promoting Tetraspanin‐mediated hemocyte phagocytosis in crab

Hemolymph exosomes inhibit Spiroplasma eriocheiris infection by promoting Tetraspanin‐mediated hemocyte phagocytosis in crab

Full-length transcriptome sequencing and comparative transcriptome analysis of Eriocheir sinensis in response to infection by the microsporidian Hepatospora eriocheir

Integrated Information for Pathogenicity and Treatment of Spiroplasma

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