ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources

Abstract

Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of antibiotics. Of the 63 bacterial isolates, 100% were resistant to carbencillin, whereas 31.74% were resistant to ticarcillin and all isolates susceptible to imipenem. In addition all of clinical isolates indicated multidrug resistant (MDR) patterns, the highest rate of MDR was observed with pattern C these isolates were able to resist (9-12) antibiotics. All isolates were typed genotypically by using two methods of amplification, ERIC and RAPD-PCR. The results of the ERIC-PCR typing of P. aeruginosa bacteria that 96.82% showed amplification bands ERIC-PCR also revealed 17 groups of genotypes (A-R) and 4 unique isolates. The results of RAPD-PCR fingerprint revealed 12 groups of genotypes (A-M) of 40–90% similarity according to coefficient values and 4 unique isolates, except 7.93% were untypeble. QS genes (lasI, lasR, rhlI, rhlR), screen showed all isolates 100% were positive for one or more QS genes, in the other hand 82.53% carrying lasI, lasR, rhlI, and rhlR, while the 15.87% carrying lasI, rhlI, and rhlR and 1.58% carrying lasI, lasR, and rhlR genes. ERIC genotyping significantly correlated resistance patterns but not with virulence control QS genes. RAPD genotyping significantly correlated with source of infection, resistance patterns and virulence control QS genes. These results can help initial diagnosis MDR P. aeruginosa outbreaks associated with specific genotyping patterns.

Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

“…RAPD-PCR, a method designed to investigate the genetic differences of bacteria isolated from different sources, is based on various primers and reaction conditions and as a method it is easy to do and cost-effective for large number of isolates (Hilton et al, 2006). In study conducted by Sallman et al (2018) in Iraq, they found that 61 P. aeruginosa strains isolated from different clinical cases showed changing similarity rates between 25-92% with RAPD-PCR. In a study conducted by Ozturk et al…”

Evaluation of Quorum-Sensing, Antibiotics Resistance, and Biofilm Formation in Pathogenic Bacteria from the Hospital Environments

“…Current findings revealed local isolates carrying one or more QS genes. The Iraqi study conducted by Sallman et al (2018) reported 82.53% isolates carrying lasI/lasR and rhlI/rhlR genes. Senturk et al (2012) displayed that 77.7%, 88.8%, 66.6%, and 77.7% of isolates were positive for rhIR, rhlI , lasR, and lasI, respectively.…”

Co-existence of LasI, RhI, and Pseudomonas Quinolone Signal Quorum-sensing Genes in Clinical Pseudomonas aeruginosa Isolates

“…These observations agree with other authors who mentioned that P. aeruginosa is present in about 33% of burn wounds and 59% of extensive burns. Huebinger RM et al 16 and Sallman RS et al 17 noticed that P. aeruginosa was obtained from 41.26% burn infection, followed by 28.57% wound swabs. In this study, it was noticed that the lowest percentage of isolation was from vaginal swab 1/50 (2%), followed by blood samples 1/50 (2%).…”