ERIC-PCR-based molecular typing of multidrug-resistant Escherichia coli isolated from houseflies (Musca domestica) in the environment of milk and meat shops

Abstract

Significance and impact of the study: Houseflies (Musca domestica) are endophilic cosmopolitan pests of medical and veterinary importance. These are associated with regions of human activities such as food centres, butcher houses, animal farms and surrounding areas and create an annoyance to people, poultry and domestic farm animals. The present study reports that housefly samples collected from milk and meat outlets were positive for multidrug-resistant (MDR) E. coli and harbour bla CTX-M and bla TEM genes in plasmid along with other resistant genes for tetracyclines and quinolones. The presence of MDR genes in bacteria isolated from housefly samples is of serious concern from public health and food safety points of view. Investigation of genetic determinants of MDR in bacteria would be of great value for formulating and validating suitable control strategies to combat the spread of antimicrobial resistance.

Značaj i razvoj metode za kontrolu pesticida u mleku iz organske proizvodnje

“…jagodama, gde je ispitivanim uzorcima potrvđeno prisistvo pesticida azoksistrobina, lambda-cihalotrina i tiametoksamina ispod MDK vrednosti (Kobi et al, 2018). Prisustvo rezidua karbarila, 4'4' DDT and deltametrin pesticida je pronađeno u 27,5%; 11% i 5% uzoraka mleka, respektivno od ispitivanih 200 uzoraka u Indiji (Chandrakar et al, 2020). Zbog tendencije opstanka pesticida duže vreme u životnoj sredini i prelaska u lanac ishrane, organska proizvodnja zahteva upotrebu biofertilizera i biopesticida.…”

Antimicrobial activity of moringa leaf infusion on Escherichia coli isolate from Musca domestica L

“…The activity of M. domestica, which lands on dirty substrates, causes the fly's body to carry various types of bacteria and transfer them to food (Rofieq et al 2020). M. domestica flies are known to act as mechanical vectors of pathogenic bacteria, including E. coli (Lindeberg et al 2018;Chandrakar et al 2022). The prevalence of diarrhea may rise if M. domestica fly populations rise (Khamesipour et al 2018).…”