Erianin suppressed lung cancer stemness and chemotherapeutic sensitivity via triggering ferroptosis

Abstract

The previous research has focused on the suppressive effects of Erianin on tumor progression, but its impact on cancer stemness has not been reported. This study aimed to investigate the effects of Erianin on lung cancer stemness. First, we screened various concentrations Erianin to ensure that it did not affect lung cancer cell viability. Subsequently, we found that Erianin significantly attenuated lung cancer stemness through various analyses, including qRT‐PCR, western blot, sphere‐formation, and ALDH activity detection. Furthermore, Erianin was shown to enhance chemosensitivity of lung cancer cells. Mechanistically, three inhibitors (cell apoptosis inhibitor, necrosis inhibitor, and ferroptosis inhibitor) were added into lung cancer cells with Erianin treatment, respectively, and we found that Erianin mainly suppressed lung cancer stemness through ferroptosis. Taken together, this study reveals that Erianin has the potential to suppress lung cancer stemness and could be a valuable chemotherapeutic enhancer for lung cancer.

Beyond Mortality: Exploring the Influence of Plant Phenolics on Modulating Ferroptosis—A Systematic Review

“…This modulation resulted in the accumulation of iron, overproduction of ROS, and cell death through ferroptosis [ 10 , 51 , 58 , 59 , 60 , 61 ]. Furthermore, amentoflavone [ 62 , 63 , 64 ], baicalin [ 54 , 55 , 65 ], erianin [ 37 , 41 , 57 , 66 , 67 ], gambogenic acid [ 68 ], auriculasin [ 69 ], wogonin [ 70 ], quercetin [ 71 , 72 ], shikonin [ 73 , 74 ], scoparone [ 75 ], osthole [ 76 ], also have shown to be able to inhibit cancer growth though the induction of ferroptosis. All of this suggests the potential for developing novel cancer treatments grounded in natural products acting as ferroptosis inducers.…”

“…Researchers are particularly interested in eranin due to its demonstrated potential for treating conditions such as inflammation, diabetic nephropathy, retinopathy, psoriasis, and various cancers. Given that ferroptosis is implicated in the development of listed conditions, it come as no surprise that eranin demonstrated potential as a modulator of ferroptosis [ 37 , 41 , 57 , 66 , 67 ].…”

Unveiling the role of YARS1 in bladder cancer: A prognostic biomarker and therapeutic target

“… 31 Moreover, induction of ferroptosis in various tumours inhibits tumour stemness. 32 , 33 , 34 , 35 It has been discovered that many of the same molecular signalling networks regulate both cellular senescence and the maintenance of stem cell stemness. Key effector signalling molecules involved in the regulation of cellular senescence, such as Bmi‐1, p16, p21, p53 and H3K9me3, are also involved in maintaining stem cell stemness.…”

Erianin promotes apoptosis and inhibits Akt-mediated aerobic glycolysis of cancer cells

“…In lung cancer, Erianin inhibits cell growth and migration via PI3K/Akt pathway 21 , and calcium/calmodulin-dependent ferroptosis 22 , 23 . Erianin suppresses lung cancer stemness via ferroptosis 24 . These studies imply that Erianin is a potent and promising antitumor agent for clinical treatment.…”